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Improved Micropropagation in Hybanthus enneaspermus L. Muell

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DOI: 10.4236/ajps.2013.46144    2,963 Downloads   4,902 Views   Citations

ABSTRACT

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

G. Premkumar, N. Arumugam, S. Muthuramkumar, G. Varatharaju and K. Rajarathinam, "Improved Micropropagation in Hybanthus enneaspermus L. Muell," American Journal of Plant Sciences, Vol. 4 No. 6, 2013, pp. 1169-1172. doi: 10.4236/ajps.2013.46144.

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