Determination of Ketoprofen in Human Plasma by RP-HPLC

Farya Zafar, Muhammad Harris Shoaib, Asia Naz, Rabia Ismail Yousuf, Huma Ali

Department of Pharmaceutical Chemistry, Faculty of Pharmacy,University of Karachi, Karachi, Pakistan.
Department of Pharmaceutics, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan.
Ziauddin College of Pharmacy, Ziauddin University, Karachi, Pakistan.

DOI: 10.4236/ajac.2013.45031                

Abstract

For in vivo pharmacokinetic studies, it is pre-requisite to quantify drug concentrations in plasma. In the present study a RP-HPLC procedure was developed and validated for the assessment of ketoprofen in human plasma. For this purpose mobile phase consisting of methaol:water (70:30) adjusted to pH 3.3 with phosphoric acid was used, and chromatography was carried out on Discovery HS C18 column, 5 μm (25 cm × 4.6 mm). The flow rate was 1 mL·min^-1 and quantitative assessment was performed at 260 nm. The retention time was found to be <10 min. A method was found to be accurate and illustrated linearity from 0.2441 to 125 μg·mL^-1 with the determination coefficient (r²) of 0.9999, also accuracy and precision were found to be <2 (%RSD). The intraday accuracy for concentrations 62.5 μg·mL^-1, 15.625 μg·mL^-1, 7.812 μg·mL^-1 and 1.953 μg·mL^-1 were found to be 99.747%, 99.475%, 98.457% and 99.824% respectively where as for interday accuracy consecutive values for days 1, 2 and 3 were 99.104%, 99.091%, 98.96% and 99.385% in plasma. All validation parameters were assessed and were found to be within the limits. The proposed method was accurate, specific, quick (retention time < 10 min), selective (showed no interference with excipients), cost effective and a good resolution which gave this method an advantage over the different other reported methods for the estimation of ketoprofen in human plasma.

Keywords

RP-HPLC; Ketoprofen; Linearity; Accuracy; Precision; Specific

Conflicts of Interest

The authors declare no conflicts of interest.

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