Share This Article:

Low and High Risk Human Papillomavirus in the Oral Mucosa of Mexican Women with Genital Papillomavirus

Full-Text HTML XML Download Download as PDF (Size:643KB) PP. 62-69
DOI: 10.4236/ojmm.2013.31010    4,181 Downloads   6,861 Views   Citations

ABSTRACT

Background: Human papillomaviruses (HPV) are implicated in cervical cancer and, recently in oral cancer. In Mexico, there are few studies on oral cancer, therefore the interest in identifying the HPV frequency of low and high risk in samples of the oral and cervical cavities, and determining some risk factors. Objective: To determine the frequency of high and low risk HPV infection in the oral cavity of women with cervical HPV, and to correlate the infection site with risk factors. Materials and Methods: Eighteen female patients between 24 and 53 years, with antecedents of genital HPV infection were included. Both samples of oral cavity and cervix were obtained. DNA extraction from the epithelial cells was performed using the Qiagen kit. PCR was done and the amplicon was observed in 2% agarose gels stained with ethidium bromide. A correlation of HPV infection and risk factors was done. Results: HPV-DNA was detected in the 67% of both samples. The frequency of oral and cervix low risk HPV-DNA was 50%, while high risk HPV-DNA in oral cavity was detected in 17%, and 39% in the cervix. The study of the risk factors involved in HPV infection showed that the participants had the habits of smoking 39%; alcohol drinking 28%; and 78% oral sex. Conclusion: The results showed a high frequency of HPV (67%) infection in the oral and genital mucosas, suggesting that patient’s habits could contribute to the infection; the presence of HPV in the oral mucosa may act as reservoir for new HPV infections.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

A. Fernández, D. Rosete, S. Pedraza, F. Mejía, J. Delgado and M. Manjarrez, "Low and High Risk Human Papillomavirus in the Oral Mucosa of Mexican Women with Genital Papillomavirus," Open Journal of Medical Microbiology, Vol. 3 No. 1, 2013, pp. 62-69. doi: 10.4236/ojmm.2013.31010.

References

[1] M. Ca?adas, F. Bosch, M. Junquera, M. Ejarque, R. Font, E. Ordo?ez and S. de Sanjosé, “Concordance of Prevalence of Human Papillomavirus DNA in Anogenital and Oral Infections in a High-Risk Population,” Journal of Clinical Microbiology, Vol. 42, No. 3, 2004, pp. 1330-1332.
[2] J. Baseman and L. Kuotsky, “The Epidemiology of Human Papillomavirus Infections,” Journal of Clinical Virology, Vol. 32, 2005, pp. 16-24. doi:10.1016/j.jcv.2004.12.008
[3] A. Hidalgo-Martìnez, “El Cáncer Cérvico-Uterino, su Impacto en Mèxico y el porqué no Funciona el Programa Nacional de Detección Oportuna,” Revista Biomédica, Vol. 17, No. 1, 2006, pp. 81-84.
[4] INEGI, “Secretaría de Salud,” Dirección General de Información en Salud con Base en Datos de Defunciones, México City, 2005.
[5] M. Manjarrez, R. Ocadiz, L. Valle, C. Checo, A. Marroquin, C. de la Torre, M. Selman and P. Gariglio, “Detection of Human Papillomavirus and Relevant Tumor Supressors and Oncoproteins in Laryngeal Tumors,” Clinical Cancer Research, Vol. 12, No. 23, 2006, pp. 6946-6951. doi:10.1158/1078-0432.CCR-06-1214
[6] K. Kurose, M. Terai, N. Soedarsono, D. Rabello, Y. Nakajima, R. Burk and M. Takagi, “Low Prevalence of HPV Infection and Its Nature History in Normal Oral Mucose among Volunteers on Miyako Island Japan,” Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics, Vol. 98, No. 1, 2004, pp. 91-96. doi:10.1016/j.tripleo.2003.12.029
[7] K. Uobe, K. Masuno Y. Fang, L. Li, Y. Wen and Y. Ueda, “Detection of HPV in Japanese and Chinese Oral Carcinoma by in Situ PCR,” Oral Oncology, Vol. 37, No. 2, 2001, pp. 146-152. doi:10.1016/S1368-8375(00)00075-0
[8] J. Kello, S. Syrj?nen, M. Yliskoski and K. Syrj?nen, “Dot Blot Hybridization in Detection of Human Papillomavirus (HPV) Infections in the Oral Cavity of Women with Genital HPV Infections,” Oral Microbiology and Immunology, Vol. 7, No. 1, 1992, pp. 19-23. doi:10.1111/j.1399-302X.1992.tb00014.x
[9] P. Castle, “Human Papillomavirus in Oral Exfoliated Cells and Risk of Head and Neck Cancer,” Journal of the National Cancer Institute, Vol. 96, No. 15, 2004, pp. 1181-1183.
[10] F. Bosch, M. Manos, N. Mu?oz, M. Sherman, A. Jansen, J. Peto, M. Schiffman, V. Moreno, R. Kurman and K. Shah, “Prevalence of Human Papillomavirus in Cervical Cancer: A Worldwide Perspective; International Biological Study on Cervical Cancer (IBSCC) Study Group,” Journal of the National Cancer Institute, Vol. 87, No. 11, 1995, pp. 796-802. doi:10.1093/jnci/87.11.796
[11] M. Schiffman, H. Bauer and R. Hoover, “Epidemiologic Evidence Showing That Human Papillomavirus Infection Causes Most Cervical Intraepithelial Neoplasia,” Journal of the National Cancer Institute, Vol. 85, No. 11, 1993, pp. 958-964. doi:10.1093/jnci/85.12.958
[12] S. M. Schwartz, J. R. Daling, D. R. Doody, G. C. Wipf, J. J. Carter, M. M. Madeleine, E. J. Mao, E. D. Fitzgibbons, S. Huang, A. M. Beckmann, J. K. McDougall and D. A. Galloway, “Oral Cancer Risk in Relation to Sexual History and Evidence of Human Papillomavirus Infection,” Journal of the National Cancer Institute, Vol. 90, No. 21, 1998, pp. 1626-1636. doi:10.1093/jnci/90.21.1626
[13] S. Nair and M. Pillai, “Human Papillomavirus and Disease Mechanisms: Relevance to Oral and Cervical Cancers,” Oral Diseases, Vol. 11, No. 6, 2005, pp. 350-359. doi:10.1111/j.1601-0825.2005.01127.x
[14] P. Giraldo, A. Goncalves S. Pereira, S. Barros-Mazon, M. Gondo and S. Witkin, “Human Papillomavirus in the Oral Mucosa of Women with Human Papillomavirus Lesions”, European Journal of Obstetrics & Gynecology and Reproductive Biology, Vol. 126, No. 1, 2006, pp. 104-106.
[15] E. de Villers, C. Fauquet, T. Broker, H. Bernand and Z. Hausen, “Classification of Papillomaviruses,” Virology, Vol. 324, No. 1, 2004, pp. 17-27. doi:10.1016/j.virol.2004.03.033
[16] M. Conway and C. Meyers, “Replication and Assembly of Human Papillomavirus,” Journal of Dental Research, Vol. 88, No. 4, 2009, pp. 307-317. doi:10.1177/0022034509333446
[17] E. Burd, “Human Papillomavirus and Cervical Cancer,” Clinical Microbiology Reviews, Vol. 16, No. 1, 2003, pp. 1-17. doi:10.1128/CMR.16.1.1-17.2003
[18] D. Chang, C. Hsieh, R. Chen, S. Lee and S. Huang, “Comparison of Detection of Human Papillomavirus 16 DNA in Cervical Carcinoma Tissues and Southern Blot Hybridisation and Nested Polymerase Chain Reaction,” Journal of Medical Microbiology, Vol. 43, No. 6, 1995, pp. 430-435. doi:10.1099/00222615-43-6-430
[19] S. Begum, M. Guillison, T. Nico and W. Westra, “Detection of Human Papillomavirus 16 in Fine-Needle Aspirates to Determine Tumor Origin in Patients with Metastatic Squamous Cell Carcinoma of the Head and Neck,” Clinical Microbiology Reviews, Vol. 13, No. 4, 2007, pp. 1186-1191. doi:10.1158/1078-0432.CCR-06-1690
[20] P. Castle, “Human Papillomavirus in Oral Exfoliated Cells and Risk of Head and Neck Cancer,” Journal of the National Cancer Institute, Vol. 96, No. 15, 2004, pp. 1181-1183. doi:10.1093/jnci/djh232
[21] T. Castro and I. F. Bussoloti, “Prevalence of Human Papillomavirus (HPV) in Oral Cavity and Oropharynx,” Revista Brasileira de Otorrinolaringologia, Vol. 72, No. 2, 2006, pp. 272-282. doi:10.1590/S0034-72992006000200021
[22] G. D’Souza, A. Kreimer, R. Viscidi, M. Pawlita, C. Fakhry, W. Koch, W. Westra and M. Guillison, “Case-Control Study of Human Papillomavirus and Oropharyngeal Cancer,” The New England Journal of Medicine, Vol. 356, No. 19, 2007, pp. 1944-1956. doi:10.1056/NEJMoa065497
[23] C. Furniss, M. McClean, J. Smith, J. Bryan, K. Applebaum, H. Nelson, M. Posner and K. Kelsey, “Human Papillomavirus 6 Seropositivity Is Associated with Risk of Head and Neck Squamous Cell Carcinoma, Independent of Tobacco and Alcohol Use,” Annals of Oncology, Vol. 20, No. 3, 2009, pp. 534-541. doi:10.1093/annonc/mdn643
[24] L. Giovannelli, G. Campisi, A. Lama, O. Giambalvo, J. Osborn, V. Margiotta and P. Ammatuna, “Human Papillomavirus DNA in Oral Mucosal Lesions,” The Journal of Infectious Diseases, Vol. 185, No. 6, 2002, pp. 833836. doi:10.1086/339193
[25] D. Hennessey, W. Westra and J. Califano, “Human Papillomavirus and Head and Neck Squamous Cell Carcinoma: Recent Evidence and Clinical Implications,” Journal of Dental Research, Vol. 88, No. 4, 2009, pp. 300-306. doi:10.1177/0022034509333371
[26] C. Fakhry, G. D’Souza, E. Sugar, K. Weber, E. Goshu, H. Minkoff, R. Wright, E. Seaberg and M. Guillison, “Relationship between Prevalent Oral and Cervical Human Papillomavirus Infections in Human Immunodeficiency Virus-positive and-negative Women,” Journal of Clinical Microbiology, Vol. 44, No. 12, 2006, pp. 4479-4485. doi:10.1128/JCM.01321-06
[27] J. Walboomers, M. Jacobs M. Manos, F. Bosch, J. Kummer, K. Shah, P. Snijders, J. Peto, C. Meije and N. Munoz, “Human Papillomavirus Is a Necessary Cause of Invasive Cervical Worldwide,” Journal of Pathology, Vol. 189, No. 1, 1999, pp. 12-19. doi:10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F
[28] Y. Fujinaga, M. Shimada, K. Okasawa, M. Fukushima, I. Kato and K. Fujinaga, “Simultaneous Detection and Typing of Genital Human Papillomavirus DNA Using the Polymerase Chain Reaction,” Journal of General Virology, Vol. 72, No. 5, 1991, pp. 1039-1044.
[29] F. Bosch, A. Lorincz, N. Mu?oz, C. Meijer and K. Shah, “The Causal Relation between Human Papillomavirus and Cervical Cancer,” Journal of Clinical Pathology, Vol. 55, No. 4, 2002, pp. 244-265. doi:10.1136/jcp.55.4.244
[30] M. Schiffman, H. Bauer and R. Hoover, “Epidemiologic Evidence Showing That Human Papillomavirus Infection Causes Most Cervical Intraepithelial Neoplasia,” Journal of the National Cancer Institute, Vol. 85, No.12, 1993, pp. 958-964. doi:10.1093/jnci/85.12.958
[31] N. Termine, L. Giovannelli, S. Matranga, A. Perino, V. Panzarella, P. Ammatuna, M. D’Angelo and G. Campisi, “Low Rate of Oral Human Papillomairus (HPV) Infection in Women Screened for Cervical HPV Infection in Southern Italy: A Cross-Sectional Study of 140 Immunocompetent Subjects,” Journal of Medical Virology, Vol. 81, No. 8,2009, pp. 1438-1443. doi:10.1002/jmv.21509
[32] G. D’Souza, E. Sugar, W. Ruby, P. Gravitt and M. Guillison, “Analysis of the Effect of DNA Purification on Detection of Human Papillomavirus in Oral Rinse Samples by PCR,” Journal of Clinical Microbiology, Vol. 43, No. 11, 2005, pp. 5526-5535. doi:10.1128/JCM.43.11.5526-5535.2005
[33] E. Smith, J. Ritchie, J. Yankowitz, D. Wang, L. Turek and T. Haugen, “HPV Prevalence and Concordance in the Cervix and Oral Cavity of Pregnant Women,” Infectious Diseases in Obstetrics and Gynecology, Vol. 12, No. 2, 2004, pp. 45-56. doi:10.1080/10647440400009896
[34] E. Franco, E. Duarte-Franco and A. Ferenczy, “Cervical Cancer: Epidemiology, Prevention and Role of Human Papillomavirus Infection,” Canadian Medical Association Journal, Vol. 164, No. 7, 2001, pp. 1017-1025.
[35] D. Miletic, S. Schiffman, V. Miletic and E. Miller, “Salivary IgA Secretion Rate in Young and Elderly Persons,” Physiology & Behavior, Vol. 60, No. 1, 1996, pp. 243248. doi:10.1016/0031-9384(95)02161-2
[36] I. Thompson, P. van der Bijl, C. van Wyk and A. van Eyk, “A Comparative Light Microscopic, Electron-Microscopic and Chemical Study of Human Vaginal and Buccal Epithelium,” Archives of Oral Biology, Vol. 46, No. 12, 2001, pp. 1091-1098. doi:10.1016/S0003-9969(01)00082-6
[37] J. Cervantes, “Aspectos Inmunológicos de la Infección por Papilomavirus Humano. Rol del Complejo Mayor de Histocompatibilidad,” Revista Medica Herediana, Vol. 14, No. 2, 2003, pp. 94-98.
[38] L. Guzmán-Rojas, J. Alcocer-González and V. MadrirMarina, “Perspectivas Para el Desarrollo de Vacunas e Inmunoterapia Contra el Cáncer Cervicouterino,” Salud Pública de México, Vol. 40, No. 1, 1998, pp. 38-46 doi:10.1590/S0036-36341998000100006
[39] K. Syrjanen, “HPV Infections and Esophageal Cancer,” Journal of Clinical Pathology, Vol. 55, No. 10, 2002, pp. 721-728. doi:10.1136/jcp.55.10.721
[40] C. Roteli-Martins, K. Panetta, V. Alves, S. Siqueira, K. Syrjanen and S. Derchain, “Cigarrete Smoking and High Risk HPV-DNA as Predisposing Factors for High-Grade Cervical Intraepithelial Neoplasia (CIN) in Young Brazilian Women,” Acta Obstetricia et Gynecologica Scandinavica, Vol. 77, No. 6, 1998, pp. 678-682. doi:10.1034/j.1600-0412.1998.770617.x
[41] A. Olsen, J. Dillner, A. Skrondal and P. Magnus, “Combined Effect of Smoking and Human Papillomavirus Type 16 in Cervical Carcinogenesis,” Epidemiology, Vol. 9, No. 3, 1998, pp. 346-349. doi:10.1097/00001648-199805000-00022
[42] F. Lewin, S. Norell, H. Johansson, P. Gastavsson, J. Wennerberg, A. Bioklund and E. Rutqvist-Lars, “Smoking Tabacco, Oral Snuff, and Alcohol in the Etiology of Squamous Cell Carcinoma of the Head and Neck: A Population-Based Case-Referent Study in Sweden,” Cancer, Vol. 82, No. 7, 1998, pp.1367-1375. doi:10.1002/(SICI)1097-0142(19980401)82:7<1367::AID-CNCR21>3.0.CO;2-3
[43] S. M. Schartz, J. R. Daling, D. R. Doody, G. C. Wipf, J. J. Carter, M. Madeleine, E.-J. Mao, E. D. Fitzgibbons, S. Huang, A. M. Beckmann, J. McDougall and D. Galloway, “Oral Cancer and Risk in Relation to Sexual History and Evidence of Human Papillomavirus Infection,” Journal of the National Cancer Institute, Vol. 90, No.21, 1998, pp. 1626-1636. doi:10.1093/jnci/90.21.1626
[44] E. Smith, M. H. Hoffman, K. Summersgill, H. Kirchnner, L. P. Turek and T. Haugen, “Human Papillomavirus and Risk of Oral Cancer,” Laryngoscope, Vol. 108, No. 7, 1998, pp. 1098-1103. doi:10.1097/00005537-199807000-00027
[45] E. Ringstrom, E. Peters, M. Hasegawa, M. Posner, M. Liu and K. Kelsey, “Human Papillomavirus Type 16 and Squamous Cell Carcinoma of the Head and Neck,” Clinical Cancer Research, Vol. 8, No. 10, 2002, pp. 31873192.
[46] G. D’Souza, Y. Agrawal, J. Halpern, S. Bodison and M. Gillison, “Oral Sexual Behaviours Associated with Prevalent Oral Human Papillomavirus Infection,” Journal of Infectious Diseases, Vol. 199, No. 9, 2009, pp. 1263-1269. doi:10.1086/597755
[47] R. Morales-Espinoza, A. Fernández-Presas, G. GonzálezValencia, G. Delgado, J. L. Méndez-Sánchez, S. FloresHernández, E. Sánchez-Quezada, L. Mu?oz-Pérez, R. Léon-Aguilar, J. Hernández-Guerrero and A. Cravioto, “Helicobacter pylori Detection in the Oral Cavity Is Strongly Associated with Gastroesophageal Disease,” Oral Microbiology and Immunology, Vol. 24, No. 6, 2009, pp. 464-468. doi:10.1111/j.1399-302X.2009.00541.x

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.