Anti-Osteoporosis and Anti-Osteoarthritis Activity of Fresh Water Snail (Viviparous bengalensis) Flesh Extract in Experimental Animal Model

Amrita Sarkar, Poulami Datta, Aparna Gomes, Subir Chandra Das Gupta, Antony Gomes

Department of Zoology, Maulana Azad College, Kolkata, India.
Indian Institute of Chemical Biology, Kolkata, India.
Laboratory of Toxinology & Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, Kolkata, India.

DOI: 10.4236/ojra.2013.31003                

Abstract

Aims: Aim was to evaluate the anti-osteoporotic and anti-osteoarthritic activity of fresh water snail (Viviparous bengalensis) (VB) flesh extract (VBE) in experimental model. Settings and Design: Experimental osteoporosis (OSP) was developed in female Wistar rats by bilateral overectomy and Osteoarthritis (OA) was developed in male Wistar rats by bacterial collagenase injection. Methods and Material: VB was collected locally and authenticated, then homogenized in 3500 rpm × 15 mins and supernatant was collected. Rats were divided into-Group-1: Sham control, Group-2: OSP/OA control, Group-3: Standard (vitD₃ 200 mg·kg ^-1; p.o., calcium i.p. 1500 mg·kg ^-1 × 15 days in OSP and indomethacin 0.25 mg·kg^-1, p.o. × 5 alternative days in OA), Group-4: VBE treated (1 gm·kg^-1; p.o. × 15 days), Group-5: VBE treated (2 mg·kg^-1; p.o. × 15 days). Anti-osteoporotic and anti-osteoarthritic activity of VBE was examined through physical, urinary and serum parameters. Statistical use: Data were expressed in terms of mean ± SEM (n = 6). ANOVA was performed, p < 0.05 considered significance. Key findings: It was observed that the body weight, ankle/ knee diameters, urinary markers hydroxyproline/glucosamine/calcium/phosphate/creatinine, serum ACP/ALP/TRAP, calcium/creatinine, cytokines (TNF-α/IL-1β/CINC-1) levels were changed significantly and restored after VBE treatment. Significance: Fresh water snail flesh extract possess anti-osteoporosis and anti-osteoarthritic activity in experimental animal models.

Keywords

Water Snail; Viviparous bengalensis; Traditional Medicine; Osteoporosis; Osteoarthritis

Conflicts of Interest

The authors declare no conflicts of interest.

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