Quantitative Determination of Click Reaction in the Presence of Glycine Derivatives and in Dilute Solution

Yui Ogasawara, Yuta Murai, Yasuko Sakihama, Yasuyuki Hashidoko, Makoto Hashimoto

Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.

DOI: 10.4236/ijoc.2012.223041                

Abstract

The click reaction is one of the latest techniques for the functional analysis of bioactive compounds and the analysis makes novel concepts and strategies for medicinal chemistry. N-methylated glycine derivatives have inhibitory activity for the click reaction in direct Cu(I) system because of decrease of Cu(I) concentration. The Cu(I) concentration recovered effectively by sodium ascorbate. Quantitative determination of click reaction at various ligand concentrations revealed that the decrease in reaction yields was observed in a substrate concentration-dependent manner.

Keywords

Click Chemistry; Quantitative Determination; Propargylglycine; Cu(I); Buffer

Conflicts of Interest

The authors declare no conflicts of interest.

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