The Effect of Plant Growth Regulator and Active Charcoal on the Development of Microtubers of Potatoes

DOI: 10.4236/ajps.2012.311185   PDF   HTML     4,949 Downloads   7,716 Views   Citations

Abstract

With the detoxicated seedling of a potato cultivation breed named “Mire” as the material, the effect of auxins CCC, 6-BA, and active carbon to microtubers of potato (Solanum tubersum L.) was investigated under the in-vitro circumstances. The result indicated the exogenous auxins improved the production and quality of microtubers of potatoes. The effect of induction can be described as CCC > CCC + 6-BA > 6-BA > CK, the number of microtubers in per flask is 8.17 > 7.67 > 7.29 > 5.46, and the number of large potatoes in per flask is 6.33 > 5.17 > 3.17 > 1. In addition, by adding 0.5‰ of active charcoal, the growth period was shortened from 25.0 days to 9.33 days on average, and the amount of larger potatoes increased 8.54%. These results benefited the growth of microtubers of potato.

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M. Peng, X. Wang and L. Li, "The Effect of Plant Growth Regulator and Active Charcoal on the Development of Microtubers of Potatoes," American Journal of Plant Sciences, Vol. 3 No. 11, 2012, pp. 1535-1540. doi: 10.4236/ajps.2012.311185.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] FAO, “Potatoes in the 1990s: Situations and Prospects of World Potato Economy,” Publications, Food and Agriculture Organization, 1998.
[2] J. He, Y. Chen and Y. S. Zhang, “Induction of Potato Microtuber in Vitro,” Journal of Yunnan University, Vol. 1, No. 23, 2001, pp. 62-64.
[3] K. Kawakami, “The Physiological Degeneration of Potato Seed Tubers and Its Control,” Potato Research, Vol. 5, No. 1, 1962, pp. 40-49. http://link.springer.com/article/10.1007%2FBF02367660
[4] Y. Kim, “In Vitro Tuber Formation from Potato,” Seoul University Publishing, Seoul, 1982.
[5] M. J. Hossain and N. Sultana, “Effect of Benzyl Amino Purine (BAP) and Chloro Choline Chloride (CCC) on in Vitro Tuberization of Potato,” Bangladesh Journal of Agriculture Research, Vol. 4, No. 23, 1998, pp. 685-690.
[6] J. Gopal, J. L. Minocha and H. S. Dhaliwal, “Microtuberization in Potato (Solanum tubersum L.),” Plant Cell Reports, Vol. 17, No. 10, 1998, pp. 794-798. doi:10.1007/s002990050485
[7] R. Estrada, P. Tovar and J. H. Dodds, “Introduction of in Vitro Tubers in a Broad Range of Potato Genotypes,” Plant Cell, Tissue and Organ Culture, Vol. 7, No. 1, 1986, pp. 3-10. doi:10.1007/BF00043915
[8] J. L. John, W. H. Courtney and D. R. Decoteau, “The Influence of Plant Growth Regulators and Light on Microtuber Induction and Formation in Dioscorea alata L. Cultures,” Plant Cell, Tissue and Organ Culture, Vol. 34, No. 3, 1993, pp. 245-252. doi:10.1007/BF00029713
[9] W. S. Hao, Y. X. Zhao and Q. H. Gao, “The Progress of Potato Microtubers Induction in China,” Inner Monglia Agricultural Science and Technology, Vol. 6, 2002, pp. 4-7.
[10] M. E. Hoque, “In Vitro Tuberization in Potato (Solanum tuberosum L.),” Plant Omics Journal, Vol. 1, No. 3, 2010, pp. 7-11.
[11] D. Sarkar and P. S. Naik, “Effect of Inorganic Nitrogen Nutrition on Cytokinin-Induced Potato Microtuber Production in Vitro,” Potato Research, Vol. 41, No. 3, 1998, pp. 211-217. doi:10.1007/BF02358191
[12] O. O. Paul, K. Claire and D. Jacques, “Effects of Reducing Sugar Concentration on in Vitro Tuber Formation and Sprouting in Yam (Dioscorea cayenensis-D. rotundata Complex),” Plant Cell, Tissue and Organ Culture, Vol. 99, No. 1, 2009, pp. 55-59. doi:10.1007/s11240-009-9575-1
[13] O. Mahmoud, C. S. Farhad and N. Paul, “Effects of Temperature Fluctuation during in Vitro Phase on in Vitro Microtuber Production in Different Cultivars of Potato (Solanum tubersum L.),” Plant Cell, Tissue and Organ Culture, Vol. 98, No. 2, 2009, pp. 213-218. doi:10.1007/s11240-009-9554-6
[14] K. Pruski, T. Astatkie, P. Duplessis, T. Lewis, J. Nowak and P. C. Struik, “Use of Jasmonate for Conditioning of Potato Plantlets and Microtubers in Greenhouse Production of Minitubers,” American Journal of Potato Research, Vol. 80, No. 3, 2003, pp. 183-193. doi:10.1007/BF02855690
[15] P. Kris, A. Tess and N. Jerzy, “Jasmonate Effects on in Vitro Tuberization and Tuber Bulking in Two Potato Cultivars (Solanum tubersum L.) under Different Media and Photoperiod Conditions,” In Vitro Cellular & Developmental Biology-Animal, Vol. 38, No. 2, 2002, pp. 203-209. http://rd.springer.com/article/10.1079/IVPIVP2001265
[16] Janet E. A. Seabrook, “Light Effects on the Growth and Morphogenesis of Potato (Solanum tubersum) in Vitro: A Review,” American Journal of Potato Research, Vol. 82, No. 5, 2005, pp. 353-367. http://rd.springer.com/article/10.1007/BF02871966
[17] T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, No. 3, 1962, pp. 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
[18] S. H. Mantell, S. Q. Haque and A. P. Whitehall, “Clonal Multiplication of Dioscorea alata L. and Dioscorea rotundata Poir. Yams by Tissue Culture,” The Journal of Horticultural Science, Vol. 53, No. 2, 1978, pp. 95-98.
[19] S. H. Mantell and S. A. Hugo, “Effects of Photoperiod, Mineral Medium Strength, Inorganic Ammonium, Sucrose and Cytokinin on Root, Shoot and Microtuber Development in Shoot Cultures of Dioscorea alata L. and D. bulbifera L. Yams,” Plant Cell, Tissue and Organ Culture, Vol. 16, No. 1, 1989, pp. 23-37. doi:10.1007/BF00044069
[20] H. Q. Wang and L. T. Xiao, “Effects of Chlorocholine Chloride on Phytohormones and Photosynthetic Characteristics in Potato (Solanum tubersum L.),” Journal of Plant Growth Regulation, Vol. 28, No. 1, 2009, pp. 21-27. doi:10.1007/s00344-008-9069-0
[21] J. Martine and C. Mario, “Effects of Some Growth Gegulator on in Vitro Tuberization in Dioscorea Alata L. ‘Brazo Fuerte’ and D. abyssinica Hoch,” Plant Cell Reports, Vol. 11, No. 1, 1992, pp. 34-38. http://www.springerlink.com/content/b52e1efd7fb94e9e/
[22] C. E. Palmer and O. E. Smith, “Cytokinins and Tuber Initiation in the Potato Solanum tubersum L.,” Nature, Vol. 221, 1969, pp. 279-280. doi:10.1038/221279a0
[23] C. S. Mauk and A. R. Langille, “Physiology of Tuberization in Solanum tubersum L.,” Plant Physiology, Vol. 62, No. 3, 1978, pp. 438-442. doi:10.1104/pp.62.3.438
[24] H. Obata-Sasamoto and H. Suzuki, “Activities of Enzymes Relating to Starch Synthesis and Endogenous Levels of Growth Regulators during Tuberization of Isolated Potato Stolons Cultured in Vitro,” Zeitschrift für Pflan-zenphysiologie, Vol. 95, No. 1, 1979, pp. 69-75. http://www.sciencedirect.com/science/article/pii/S0044328X7980029X
[25] G. Hussey and N. J. Stacey, “Factors Affecting the Formation of in Vitro Tubers of Potato (Solanum tuberosum L.),” Annals of Botany, Vol. 53, No. 4, 1984. pp. 565-578. http://aob.oxfordjournals.org/content/53/4/565.short
[26] S. X. Bai, Z. M. An, X. Z. Feng and J. Wang, “Studies on the Factors Affecting the Induction of in Vitro Potato Microber,” China Potato, Vol. 5, No. 15, 2001, pp. 271-273.
[27] H. Y. Yang, D. Wang and X. Pan, “Effect of Hormone and Culture Methods on Potato Microtuber Induction,” Journal of Gansu Agricultural University, Vol. 2, 2008, pp. 74-77.
[28] S. Pang, G. N. Fang, J. X. Li and J. X. Zhao, “Effect of Kinds Growth Regulator on Potato Micro-Tubers Induction,” Journal of Changjiang Vegetables, Vol. 20, 2009, pp. 25-27.
[29] C. Z. Xu, X. J. Zhang and A. J. Liu, “Effect of Activated Chroal on Explant Growth and Tuberization in Potato,” Review of China Agricultural Science and Technology, Vol. 5, 2003, pp. 106-107.
[30] R. X. Liu, “Effect of Activated Charcoal and Inorganic Nutrition on the Induction of the Test-Tube Potato,” Plant Physiology Journal, Vol. 37, 2001, pp. 295-298.
[31] Y. Luo, H. Tian and T. Zhang, “Effect of Coumarin on Microtuber Induction in Potato,” China Potato, Vol. 1, No. 14, 2000, pp. 4-8.

  
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