In vitro evaluation of antioxidant and antibacterial activities of extracts of Hertia cheirifolia’s leaves

Houes Bousselsela, Afaf Benhouda, Mouloud Yahia, Souhila Benbia, Abdelmoudjib Ghecham, Abla Zidani

Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology Departement of Biology, University of Batna, Batna City, Algeri.
Biotechnology’s Laboratory of the Bioactive Molecules and the Cellular Physiopathology Departement of Biology, University of Batna, Batna City, Algeria.

DOI: 10.4236/ns.2012.411110                

Abstract

In this study, our objective is to investigate the antioxidant activity by the means of two methods: the β-carotene bleaching method and DPPH assay as well as testing the antibacterial activity by the Agar-well diffusion method, of the extracts (EEp, EDm, EMe and EAq) from the sheets of Hertia cheirifolia. The quantitative analysis are showed that the highest content of total phenolic was concentrated in the methanolic extract with 30.33 + 2.82 μg EAG/mg of extracts, in the second level the EAq extract and EDm extract with 25.92 + 7.19 μg EAG/mg of extracts and 21.25 +1.76 μg EAG/mg of extracts respectively. The content of polyphenols was determined specrophotometrically and showed the presence of these compounds in all extracts. The analysis by TLC revealed the presence of quercetin in the EMe extract of this plant. In the β-carotene bleaching test, the EMe of this plant displayed highest antioxidant activity (72.97%), than in the DPPH assay with a radical-scavenging activity (72.74%). Our results of the antibacterial activity showed the inefficiency of the whole extracts against most of the tested strains.

Keywords

Hertia Cheirifolia; Polyphenols; Antioxidant Activity; Antibacterial Activity

Conflicts of Interest

The authors declare no conflicts of interest.

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