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Acceleration of Hericium erinaceum mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source

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DOI: 10.4236/abb.2012.37103    2,649 Downloads   4,449 Views   Citations

ABSTRACT

The effects of various carbon sources and their initial concentrations on mycelia production by Hericium erinaceum were investigated by determining the dry cell weight (DCW) and β-glucan content of mycelia in submerged culture. Glucose and xylose were superior carbon sources for promoting mycelial growth resulting in mycelial concentrations of 3.99 g/L and 4.01 g/L, respectively; glucose was the best carbon source in terms of productivity (0.44 g/L/day). Experiments were also performed using yogurt whey as an alternative nitrogen source for submerged cultivation of H. erinaceum mycelia, and DCW and β-glucan content were compared with those with chemical nutrient medium. When whey was used as a nitrogen source, DCW and total amount of β-glucan were 2.3- and 2.8-fold higher, respectively, than that with chemical nutrient medium. Thus, whey appears to be an alternative nitrogen source for promoting H. erinaceum mycelial growth.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Asada, C. , Okumura, R. , Sasaki, C. and Nakamura, Y. (2012) Acceleration of Hericium erinaceum mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source. Advances in Bioscience and Biotechnology, 3, 828-832. doi: 10.4236/abb.2012.37103.

References

[1] Kurosumi, A., Kobayashi, F., Mtui, G. and Nakamura, Y. (2006) Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochemical Engineering Journal, 30, 109-113. Hdoi:10.1016/j.bej.2006.02.004
[2] Malinowska, E., Krzyczkowski, W., Lapienis, G. and Herold, F. (2009) Improved simultaneous production of mycelial biomass and polysaccharides by submerged culture of Hericium erinaceum: Optimization using a central composite rotatable design (CCRD). Journal of Industrial Microbiology & Biotechnology, 36, 1513-1527. Hdoi:10.1007/s10295-009-0640-x
[3] Mizuno, T., Wasa, T., Ito, H., Suzuki, C. and Ukai, N. (1992) Antitumor-active polysaccharides isolated from the fruiting body of Hericium erinaceum, an edible and medicinal mushroom called yamabushitake or houtou. Bioscience Biotechnology Biochemistry, 56, 347-348. Hdoi:10.1271/bbb.56.347
[4] Ben-Hassan, R.M. and Ghaly, A.E. (1994) Continuous propagation of Kluyveromyces fragilis in cheese whey for pollution potential reduction. Applied Biochemistry and Biotechnology, 47, 89-105. Hdoi:10.1007/BF02788678
[5] Mawson, A.J. (1994) Bioconversions for whey utilization and waste abatement. Bioresource Technology, 47, 195-203. Hdoi:10.1016/0960-8524(94)90180-5
[6] Fournier, D., Schwitzguebel, J.P. and Peringer, P. (1993) Effect of different heterogeneous inocula in acidogenic fermentation of whey permeate. Biotechnology Letters, 15, 627-632. Hdoi:10.1007/BF00138553
[7] Crisiani-Urbina, E., Netzahuatl-Munoz, A.R., Manriquez-Rojas, F.J., Juárez-Ramírez, C., Ruiz-Ordaz, N. and Galíndez-Mayer, J. (2000) Batch and fed-batch cultures for the treatment of whey with mixed yeast cultures. Process Biochemistry, 35, 649-657. Hdoi:10.1016/S0032-9592(99)00116-8
[8] Kassa, A., Brownbridge, M., Parker, B.L., Skinner, M., Gouli, V., Gouli, S., Guo, M., Lee, F. and Hata, T. (2008) Whey for mass production of Beauveria bassiana and Metarhizium anisopliae. Mycological Research, 112, 583-591. Hdoi:10.1016/j.mycres.2007.12.004
[9] Lee, H., Song, M. and Hwang, S. (2003) Optimizing bio-conversion of deproteinated cheese whey to mycelia of Ganoderma lucidum. Process Biochemistry, 38, 1685-1693. Hdoi:10.1016/S0032-9592(02)00259-5
[10] Lee, H., Song, M., Yu, Y. and Hwang, S. (2003) Production of Ganoderma lucidum mycelia using cheese whey as an alternative substrate: response surface analysis and biokinetics. Biochemical Engineering Journal, 15, 93-99. Hdoi:10.1016/S1369-703X(02)00211-5
[11] Kim, S.W., Hwang, H.J., Xu, C.P., Sung, J.M., Choi, J. W. and Yun, J.W. (2003) Optimization of submerged culture process for the production of mycelial biomass and exopolysaccharides by Cordyceps militaris C738. Journal of Applied Microbiology, 94, 120-126. Hdoi:10.1046/j.1365-2672.2003.01754.x
[12] Kim, H.O., Lim, J.M., Joo, J.H., Kim, S.W., Hwang, H.J., Choi, J.W. and Yun, J.W. (2005) Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Bioresource Technology, 96, 1175-1182. Hdoi:10.1016/j.biortech.2004.09.021

  
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