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Preparation of Micron-Sized Di-Functional Magnetic Composite Polymer Particles

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DOI: 10.4236/msa.2010.13019    5,314 Downloads   10,200 Views   Citations

ABSTRACT

In this investigation micron-sized monodisperse magnetic composite polymer particles with amino and amide functional groups were prepared considering their applications in biotechnology. First, polystyrene/poly (acrylic acid-acrylam- ide-N-N-methylene-bis-acrylamide) [PS/P(AA-AAm-MBAAm)] composite polymer particles were prepared by seeded copolymerization. The carboxyl groups present on or near the particles surface were modified by amine-nucleophile, ethylene diamine (EDA), through pre-activation with dicyclohexyl carbodiimide as coupling agent. The aminated particles were then magnetically modified and named as aminated-Fe3O4 composite particles. Formation of such magnetic composite particles was confirmed by scanning electron micrographs, FTIR-spectra and magnetic susceptibility measurement. The produced composite particles were paramagnetic. To see the relative hydrophilic character of the particles surface the adsorption behavior of trypsin (TR) as biomolecule was studied on PS particles and aminated-Fe3O4 composite particles. The magnitude of adsorbed TR on PS particles was higher than that on aminated-Fe3O4 composite particles.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

H. Ahmad, T. Tofaz, M. Oli, M. Rahman, M. Miah and K. Tauer, "Preparation of Micron-Sized Di-Functional Magnetic Composite Polymer Particles," Materials Sciences and Applications, Vol. 1 No. 3, 2010, pp. 109-117. doi: 10.4236/msa.2010.13019.

References

[1] C. Picot, “Position Paper: Functional Polymer Latexes,” Polymer for Advanced Technologies, Vol. 6, No. 7, 1995, pp. 427-434.
[2] Q. Wang, S. Fu and T. Yu, “Emulsion Polymerization,” Progress in Polymer Science, Vol. 19, No. 4, 1994, pp. 703-753.
[3] J-W. Kim, J-G. Park, J-H. Ryu, I-S. Chang and K-D. Suh, “Monodisperse Chloromethyl-Functionalized Macropor- ous Polymer Particles by Seeded Polymerization in Aqueous Media,” Colloid and Polymer Science, Vol. 283, No. 11, 2005, pp. 1233-1240.
[4] M-O. Jung, J-H. Ryu, J-G Park, J-B. Jun and K-D. Suh, “Surface Modification of Monodisperse-Crosslinked Polymeric Microspheres Using a Redox Initiation Sys- tem,” Journal of Applied Polymer Science, Vol. 100, No. 2, 2006, pp. 1349-1356.
[5] H. Ahmad, M. M. Rahman and M. A. J. Miah, “Prepara- tion of Micron-sized Epoxy Functionalized Polystyrene Particles and Adsorption of Biomolecules,” Journal of Polymer Materials, Vol. 21, No. 1, 2005, pp. 27-36.
[6] B. Charleux, C. Pichot and M. F. Llauro, “Styrene- terminated Poly(vinyl alcohol) Macromonomers: 2. Free- radical (Co)polymerization Studies and Application to the Functionalization of Latex Particles,” Polymer, Vol. 34, No. 2, 1993, pp. 4352-4359.
[7] H. Ahmad, M. A. J. Miah, M. S. Pervin and M. M. Rah- man, “Composite Polymer Particles Prepared with Dif- ferent Amounts of Acrylic Acid and Their Usefulness as Carriers for Biomolecules,” Colloid and Polymer Science, Vol. 281, No. 10, 2003, pp. 988-992.
[8] A. M. I. Ali, P. Pareek, L. Sewell, A. Schmid, S. Fujii, S. P. Armes and I. M. Shirley, “Synthesis of Poly (2-hy- droxypropyl methacrylate) Latex Particles Via Aqueous Dispersion Polymerization,” Soft Matter, Vol. 3, No. 5, 2007, pp. 1003-1013.
[9] S. Margel and E. Wiesel, “Acrolein Polymerization: Mon- odisperse, Homo and Hybrido Microspheres, Synthesis, Mechanism, and Reactions,” Journal of Polymer Science, Polymer Chemistry Edition, Vol. 22, No. 1, 1984, pp. 145- 158.
[10] M. Okubo, Y. Kondo and M. Takahashi, “Production of Submicron-Size Monodisperse Polymer Particles Having Aldehyde Groups by Seeded Aldol Condensation,” Col- loid and Polymer Science, Vol. 271, No. 2, 1993, pp. 109- 113.
[11] P. Lei, J. Xu, Q. Wang and C. Wu, “Surface Functionali- zation of Polymer Latex Particles: 4. Tailor-Making of Emulsifier-Free System, Langmuir, Vol. 16, No. 9, 2000, pp. 4141-4147.
[12] K. Kang, C. Kan, Y. Du and D. Liu, “Synthesis and Properties of Soap-Free Poly(Methyl Methacrylate-Ethyl Acrylate-Methacrylic Acid) Latex Particles Prepared by Seeded Emulsion Polymerization,” European Polymer Journal, Vol. 41, No. 3, 2005, pp. 439-445.
[13] X. Z. Kong and E. Ruckenstein, “Monodisperse Core/ shell Latex Particles Containing Carboxylic Acid Groups and their Optimum Acid Content for Pore Generation,” Journal of Applied Polymer Science, Vol. 71, No. 9, 1999, pp. 1455-1460.
[14] J-S. Song, L. Chagai and M. A. Winnik, “Monodisperse Micrometer-size Carboxyl-functionalized Polystyrene Particles Obtained by Two-Stage Dispersion Polymeriza- tion,” Macromolecules, Vol. 19, No. 17, 2006, pp. 5729- 5737.
[15] H. Ahmad, M. M. Hossain, M. M. Rahman and M. A. J. Miah, “Monodispersed Carboxylated Composite Polymer Microspheres and Physical Immobilization of Bio- molecules,” Polymer Journal, Vol. 39, No. 5, 2007, pp. 428-434.
[16] T. Ding, E. S. Daniels, M. S. El-Aasser and A. Klein, “Synthesis and Characterization of Functionalized Poly- mer Latex Particles through a Designed Semicontinuous Emulsion Polymerization Process,” Journal of Applied Polymer Science, Vol. 97, No. 1, 2005, pp. 248-256.
[17] T. Delair, V. Marguet, C. Pichot and B. Mandrand, “Syn- thesis and Characterization of Cationic Amino Function- alized Polystyrene Latexes,” Colloid and Polymer Sci- ence, Vol. 272, No. 8, 1994, pp. 962-970.
[18] I. Miraballes-Martínez, A. Martín-Molina, F. Galisteo- González and J. Forcada, “Synthesis of Amino-Function- alized Latex Particles by a Multistep Method,” Journal of Polymer Science Polymer Chemistry Edition, Vol. 39, No. 17, 2001, pp. 2929-2936.
[19] Z. Ma, Y. Guan and H. Liu, “Synthesis and Characteriza- tion of Micron-Sized Monodisperse Superparamagnetic Polymer Particles with Amino Groups,” Journal of Poly- mer Science Polymer Chemistry, Vol. 43, No. 15, 2005, pp. 3433-3439.
[20] D. Horace and P. Caporal, “Reactive Poly(Lucidly Meth- acrylate) Microspheres Prepared by Dispersion Polymeri- zation,” Journal of Polymer Science Polymer Chemistry, Vol. 38, No. 21, 2000, pp. 3855-3863.
[21] J. M. Jin, J. M. Lee, M. H. Ha, K. Lee and S. Choe, “Highly Crosslinked Poly(Glycidyl Methacrylate-co-Di- vinyl Benzene) Particles by Precipitation Polymeriza- tion,” Polymer, Vol. 48, No. 11, 2007, pp. 3107-3115.
[22] B. Gong, J. Zhu, L. Li, K. Qiang and L. Ren, “Synthesis of Non-Porous Poly(Glycidyl Methacrylate-co-Ethylene- Dimethacrylate) Beads and their Application in Separa- tion of Biopolymers,” Talanta, Vol. 68, No. 3, 2006, pp. 666-672.
[23] X. Lu, D. Huang, X. Yang and W. Huang, “Preparation of Narrow or Monodisperse Polymer Microspheres with Cyano Group by Distillation-Precipitation Polymeriza- tion,” Polymer Bulletin, Vol. 56, No. 2-3, 2006, pp. 171- 178.
[24] T. Delair, C. Pichot and B. Mandrand, “Synthesis and Characterization of Cationic Latex Particles Bearing Sul- fhydryl Groups and their Use in the Immobilization of Fab Antibody Fragments (1),” Colloid and Polymer Sci- ence, Vol. 272, No. 1, 1994, pp. 72-81.
[25] T. Bahar and A. Tuncel, “Monodisperse Poly (p-chloro- methylstyrene) Microbeads by Dispersion Polymeriza- tion,” Polymer Engineering and Science, Vol. 39, No. 10, 1999, pp. 1849-1855.
[26] H. Ahmad, N. Saito, Y. Kagawa and M. Okubo, “Prepa- ration of Micrometer-Sized Monodisperse ‘Janus’ Com- posite Polymer Particles Having Temperature-sensitive Polymer Brushes at Half of the Surface by Seeded Atom Transfer Radical Polymerization,” Langmuir, Vol. 24, No. 3, 2008, pp. 688-691.
[27] S. Baruch-Sharon and S. Margel, “Preparation and Char- acterization of Core-Shell Polystyrene/Polychloromethyl- styrene and Hollow Polychloromethylstyrene Microme- ter-sized Particles of Narrow-size Distribution,” Colloid and Polymer Science, Vol. 287, No. 7, 2009, pp. 859-869.
[28] K. E. J. Barrett, “Dispersion Polymerization in Organic Meidia,” British Polymer Journal, Vol. 5, No. 4, 1973, pp. 259-271.
[29] C. M. Tseng, Y. Y. Lu, M. S. El-Aasser and J. W. Van- derhoff, “Uniform Polymer Particles by Dispersion Po- lymerization in Alcohol,” Journal of Polymer Science Polymer Chemistry Edition, Vol. 24, No. 11, 1986, pp. 2995-3007.
[30] T. Okaya, K. Kikuchi, A. Sukuki and N. Ikeda, “Disper- sion Polymerization of Vinyl Acetate in a Mixture of Ethanol and Water,” Polymer International, Vol. 54, No. 1, 2005, pp. 143-148.
[31] D. Hérault, C. Saluzzo and M Lemaire, “Preparation of Monodisperse Enantiomerically Pure Glycidyl Methacry- late–Ethylene Glycol Dimethacrylate Copolymers in Dispersion Copolymerization: Functionalization,” Reac- tive and Functional Polymers, Vol. 66, No. 5, 2006, pp. 567-577.
[32] S. Jiang, E. D. Sudol, V. L. Dimonie and M. S. El-Aasser, “Kinetics of Dispersion Polymerization of Methyl Methacrylate and n-Butyl Acrylate: Effect of Initiator Concentration, Macromolecules, Vol. 40, No. 14, 2007, pp. 4910-4916.
[33] H. Ahmad, B. M. Chaki, M. M. Rahman, M. A. J. Miah and K. Tauer, “Solvency Effect of the Dispersion Me-dium on the Radical Polymerization of Styrene in Non-Aqueous Dispersion Media,” E-Polymers, No. 080, 2007. http://www.e-polymers.org
[34] J. S. Song, F. Tronc and M. A. Winnik, “Two-Stage Dis- persion Polymerization toward Monodisperse, Controlled Micrometer-Sized Copolymer Particles,” Journal of American Chemical Society, Vol. 126, No. 21, 2004, pp. 6562-6563.
[35] M. Yasuda, H. Seki, H. Yokoyama, H. Ogino, K. Ishimi and H. Ishikawa, “Simulation of a Particle Formation Stage in the Dispersion Polymerization of Styrene” Mac- romolecules, Vol. 34, No. 10, 2001, pp. 3261-3270.
[36] H. Ahmad, M. E. Hossain, M. A. Rahman, M. M. Rah- man, M. A. J. Miah and K. Tauer, “Carboxyl Functional- ized Poly(Methyl Methacrylate-Acrylic Acid-Ethylene Glycol Dimethacrylate) Copolymer Particles and Their Amination with Amine-Nucleophiles,” E-Polymers, No. 096. 2008. http//www.e-polymers.org
[37] P. Kronick and R. W. Gilpin, “Use of Superparamagnetic Particles for Isolation of Cells,” Journal of Biochemical and Biophysical Methods, Vol. 12, No. 1-2, 1986, pp. 73-80.
[38] X. Li and Z. H. Sun, “Synthesis of Magnetic Polymer Microspheres and Application for Immobilization of Pro- teinase of Balillus Sublitis,” Journal of Applied Polymer Science,” Vol. 58, No. 11, 1995, pp. 1991-1997.
[39] X. Liu, Y. Guan, R. Shen and H. Liu, “Immobilization of Lipase onto Micron-Size Magnetic Beads,” Journal of Chromatography B, Vol. 822, No. 1-2, 2005, pp. 91-97.
[40] T. K. Jain, M. A. Morales, S. K. Sahoo, D. L. Seslie- Pelecky and V. Labhasetwar, “Iron Oxide Nanoparticles for Sustained Delivery of Anticancer Agents,” Molecular Pharmaceutics, Vol. 2, No. 3, 2005, pp. 194-205.
[41] A. M. Schmidt, “Thermoresponsive Magnetic Colloids,” Colloid and Polymer Science, Vol. 285, No. 9, 2007, pp. 953-966.
[42] A. Elaissari and V. Bourrel, “Thermosensitive Magnetic Latex Particles for Controlling Protein Adsorption and Desorption,” Journal of Magnetism and Magnetic Mate- rials, Vol. 225, No. 1-2, 2001, pp. 151-155.
[43] F. Sayer, G. Güven and E. Pi?kin, “Magnetically Loaded Poly(methyl methacrylate-co-acrylic acid) Nano- particles,” Colloid and Polymer Science, Vol. 284, No. 9, 2006, pp. 965-978.
[44] M. Lattuada and T. A. Hatton, “Functionalization of Monodisperse Magnetic Nanoparticles,” Langmuir, Vol. 23, No. 4, 2007, pp. 2158-2168.
[45] J-J. Yuan, S. P. Armes, Y. Takabayashi, K. Prassides, C. A. P. Leite, F. Galembeck and A. L. Lewis, “Synthesis of Biocompatible Poly[2-(methacryloyloxy)ethyl phos- phorylcholine]-Coated Magnetite Nanoparticles, Lang- muir, Vol. 22, No. 26, 2006, pp. 10989-10993.
[46] R. A. Wassel, B. Grady, R. D. Kopke and K. J. Dormer, “Dispersion of Super Paramagnetic Iron Oxide Nanopar- ticles in Poly(d,l-lactide-co-glycolide) Microparticles,” Colloids and Surfaces A: Physicochemical and Engineer- ing Aspects, Vol. 292, No. 2-3, 2007, pp. 125-130.
[47] H. Mouaziz, S. Braconnot, F. Ginot and A. Ela?ssari, “Elaboration of Hydrophilic Aminodextran Containing Submicron Magnetic Latex Particles,” Colloid and Poly- mer Science, Vol. 287, No. 3, 2009, pp. 287-297.
[48] H. Mouaziz, R. Veyret, A. Theretz, F. Ginot and A. Ela?ssari, “Aminodextran Containing Magnetite Nanopar- ticles for Molecular Biology Applications: Preparation and Evaluation,” Journal of Biomedical Nanotechnology, Vol. 5, No. 2, 2009, pp. 172-181.
[49] F. Sauzedde, A. Elaissari and C. Pichot, “Hydrophilic Magnetic Polymer Latexes. 1. Adsorption of Magnetic Iron Oxide Nanoparticles onto Various Cationic Latexes,” Colloid and Polymer Science, Vol. 277, No. 9, 1999, pp. 846-855.
[50] E. Pollert, K. Kní?ek, M. Mary?ko, A. Lan?ok, J. Bo- há?ek, D. Horák and M. Babi?, “Magnetic Poly(glycidyl methacrylate) Microspheres Containing Maghemite Pre- pared by Emulsion Polymerization,” Journal of Magnet- ism and Magnetic Materials Vol. 306, No. 2, 2006, pp. 241-247.
[51] C. Yang, H. Liu, Y. Guan, J. Xing, J. Liu and G. Shan, “Preparation of Magnetic Poly(Methylmethacrylate- Divinylbenzene-Glycidylmethacrylate) Microspheres by Spraying Suspension Polymerization and their Use for Protein Adsorption,” Journal of Magnetism and Magnetic Materials, Vol. 293, No. 1, 2005, pp. 187-192.
[52] D. Horak, M. Babic, H. Mackova and M. J. Benes, “Preparation and Properties of Magnetic Nano- and Mi- crosized Particles for Biological and Environmental Separations,” Journal of Separation Science, Vol. 30, No. 11, 2007, pp. 1751-1772.
[53] D. Horak, M. Babic, H. Mackova and M. J. Benes, “Preparation and Properties of Magnetic Nano- and Mi- crosized Particles for Biological and Environmental Separations,” Journal of Separation Science, Vol. 30, No. 11, 2007, pp. 1751-1772.
[54] C. C. Berry, S. Wells, S. Charles and A. S. G. Curtis, “Dextran and Albumin Derivatised Iron Oxide Nanopar- ticles: Influence on Fibroblasts in Vitro,” Biomaterials, Vol. 24, No. 25, 2003, pp. 4551-4557.
[55] A. L. Autenshlyus, N. A. Brusenstov and A. Lockshim, “Magnetic-Sensitive Dextran-Ferrite Immunosorbents (for Diagnostic and Therapy),” Journal of Magnetism and Magnetic Materials, Vol. 122, No. 1-3, 1993, pp. 360- 363.
[56] D. Charmot, “Preparation of Monodisperse, Magnetizable, Composite Metal/Polymer Microspheres,” Progress in Colloid and Polymer Science, Vol. 79, 1989, pp. 94-100.
[57] L. P. Ramirez and K. Landfester, “Magnetic Polystyrene Nanoparticles with a High Magnetite Content Obtained by Miniemulsion Processes,” Macromolecular Chemistry and Physics, Vol. 204, No. 1, 2003, pp. 22-31.
[58] S. Lu and J. Forcada, “Preparation and Characterization of Magnetic Polymeric Composite Particles by Minie- mulsion Polymerization,” Journal of Polymer Science Polymer Chemistry Edition, Vol. 44, No. 13, 2006, pp. 4187-4203.
[59] F. Caruso, A. S. Susha, M. Giersig and H. Mohwald, “Magnetic Core-shell Particles: Preparation of Magnetite Multilayers on Polymer Latex Microspheres,” Advanced Materials, Vol. 11, No. 11, 1999, pp. 950-953.
[60] S.-Z. Hsiao, J.-L. Ou, Y. Sung, C.-P. Chang and M.-D. Ger, “Preparation of Sulfate- and Carboxyl-functionalized Magnetite/Polystyrene Spheres for Further Deposition of Gold Nanoparticles,” Colloid and Polymer Science, Vol. 288, No. 7, 2010, pp. 787-794.
[61] D. L. Pavia, G. M. Lampman, G. S. Kriz and J. A. Vy- vyan, “Introduction to Spectroscopy,” Harcourt Brace College Publishers, New York, 2008.
[62] J. L. Zhang, R. S. Srivastava and R. D. K. Misra, “Core-Shell Magnetite Nanoparticles Surface Encapsu-lated with Smart Stimuli-Responsive Polymer: Synthesis, Characterization, and LCST of Viable Drug-targeting De-livery System,” Langmuir, Vol. 23, No. 11, 2007, pp. 6342-6351.
[63] M. Ma, Y. Zhang, W. Yu, H. Y. Shen, H. Q. Zhang and N. Gu, "Preparation and Characterization of Magnetite Nanoparticles Coated by Amino Silane,” Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 212, No. 2-3, 2003, pp. 219-226.
[64] P. Govindaiah, Y. J. Jung, J. M. Lee, T-J. Park, D. Y. Ryu, J. H. Kim and I. W. Cheong, “Monodisperse and Fluo- rescent Poly(Styrene-co-Methacrylic Acid-co-2-Naphthyl Methacrylate)/Fe3O4 Composite Particles,” Journal of Colloid and Interface Science, Vol. 343, No. 2, 2010, pp. 484-490.

  
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