An Improved System for Shoot Regeneration from Stem Explants of Lombardy Poplar (Populus nigra L. var. italica Koehne)

Kamal Kanti Biswas, Takeshi Mohri, Satoshi Kogawara, Yoshihiro Hase, Yutaka Oono

Forestry and Forest Products Research Institute (FFPRI), Tsukuba, Japan..
Medical and Biotechnological Application Division, Japan Atomic Energy Agency (JAEA), Takasaki, Japan.

DOI: 10.4236/ajps.2012.39143                

Abstract

We developed a system for the regeneration of Lombardy poplar (Populus nigra L. var. italica) shoots from internodal stem explants. Using this system, shoots regenerated from 87% of the stem explants placed on Murashige and Skoog (MS) medium supplemented with 0.1 mg/L indole-3-acetic acid and 0.5 mg/L benzylaminopurine without undergoing callus formation. About 80% of the in vitro regenerated shoots developed roots on MS medium supplemented with 0.5 mg/L indole-3-butyric acid and 0.02 mg/L 1-naphthylacetic acid. Well-rooted seven-to eight-week-old regenerated plants could be transferred to soil for further growth and the survival rate of such plants after three weeks was 88%. The protocol presented here is simple and economical because it does not rely on pre-incubation in callus induction medium or repeated subculture in shoot induction medium containing trans-zeatin, an expensive substance. The in vitro regeneration system presented here could be used for evaluation of radiation sensitivity for Lombardy poplar tissues.

Keywords

Lombardy Poplar; Shoot Regeneration; Stem Explants; Auxin and Benzylaminopurine; Radiation Sensitivity

Conflicts of Interest

The authors declare no conflicts of interest.

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