Response of hydrogenase enzymes (ALDH and ADH) in Trapa natans under physiological stress

Neha Pathania-Gagandeep Kaur, Joginder Singh, Ashish Vyas, Manoj Kumar

Department of biotechnology, Lovely Professional University, Punjab (Phagwara), India.

DOI: 10.4236/abb.2012.34043                

Abstract

The present study screens activity of different enzymes in Trapa natans under aerobic and anaerobic physiological conditions. Experimental analysis was carried out to assay the activities of ALDH and ADH in the seed extract of T. natans. These enzymes are associated with the alcohol metabolism of the T. natans. It was observed that a metabolic pathway shifts from aerobic respiration to anaerobic fermentation under anoxic conditions. Physiological stress related response in trapa seeds gives the leads to understand the other model for better fermentable products. During the experiment it was found that there is difference between the concenteration of enzymes in anaerobic and aerobic conditions. Concentration was check with the help of spectrophotometer at 340 nm.

Keywords

ALDH; ADH; Anoxic Condition; Trapa natans

Conflicts of Interest

The authors declare no conflicts of interest.

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