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The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana

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DOI: 10.4236/ajmb.2012.23025    3,856 Downloads   6,548 Views   Citations


We have previously described a simple method to analyze the chromatin structure of Arabidopsis telomeres independently of that of Interstitial Telomeric Sequences (ITSs). By using this method, we found that, whereas ITSs are heterochromatic, Arabidopsis telomeres exhibit euchromatic features [1]. Some concerns have been recently raised about the accuracy of this procedure [2]. Here, we summarize these concerns and justify our experimental approaches and interpretation of results.

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The authors declare no conflicts of interest.

Cite this paper

Vaquero-Sedas, M. and Vega-Palas, M. (2012) The restriction endonuclease Tru9I is a useful tool to analyze telomere sequences separately from interstitial telomeric sequences in Arabidopsis thaliana. American Journal of Molecular Biology, 2, 242-244. doi: 10.4236/ajmb.2012.23025.


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[7] Vannier, J., Depeiges, A., White, C. and Gallego, M. (2009) ERCC1/XPF protects short telomeres from homologous recombination in Arabidopsis thaliana. PLoS Genetics, 5, e1000380. doi:10.1371/journal.pgen.1000380
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