Sterilization of Hibiscus rosa-sinensis L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on In Vitro Direct Regeneration

Chew Tiong Dar, Janna Ong Abdullah, Parameswari Namasivayam, Siti Habsah Roowi

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Ser-dang, Malaysia.
Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Malaysia.
PT Lengkuk Technology, Felda Biotechnology Centre, Nilai, Malaysia..

DOI: 10.4236/ajps.2012.36095                

Abstract

This paper reports on the effects of organic ingredients in facilitating direct shoot regeneration from nodal explants of Hibiscus rosa-sinensis L. This paper also compares the sterilization conditions for 3 types of explants (node, internode, and shoot tip) harvested from an open field. The optimized sterilization conditions for the explants were 40% Clorox- 20 min exposure, 10% Clorox-15 min exposure, and 5% Clorox-40 min exposure for the node, internode and shoot tip, respectively. In the direct shoot regeneration using the nodal explants, we found MS medium containing 40 g/L sucrose, 0.3% (w/v) activated charcoal, and supplementations with myo-inositol, thiamine and nicotinic acid were suitable. The in vitro shoot survival rate was 30% with a mean leaf numbers of 2.68 produced, and a mean leaf length of 1.71 cm achieved after 5 weeks of culture on the modified medium.

Keywords

Hibiscus rosa-sinensis L.; Murashige and Skoog Medium; Axillary Bud; Sucrose; Organic Ingredient; Activated Charcoal

Conflicts of Interest

The authors declare no conflicts of interest.

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