Irr1/Scc3 cohesin interacts with Rec8 in meiotic prophase of Saccharomyces cerevisiae

Agata Cena, Piotr Kowalec, Danuta Płochocka, Anna Kurlandzka

Institute of Biochemistry and Biophysics, Polish Academy of Sciences Pawinskiego 5A 02-106 Warszawa, Poland.

DOI: 10.4236/ojgen.2012.21001                

Abstract

The meiotic cohesin complex of S. cerevisiae shares with the mitotic one the Irr1/Scc3, Smc1, and Smc3 subunits, while the meiosis-specific subunit Rec8 replaces mitotic subunit Scc1/Mcd1. We noticed earlier that the irr1-1 mutation (F658G) severely affected meiosis. The irr1 1/IRR1 cells were entering meiosis before having completed mitotic cell division. Using meiotic two-hybrid assay and co-immunoprecipitation we show that in cells arrested in pachytene due to a lack of a gene-regulatory factor Ndt80, the Irr1 protein interacts with Rec8p and the irr1-1 mutation abolishes this interaction. These findings indicate an important role of Irr1p in early stages of meiosis.

Keywords

Irr1/Scc3; Cohesin; Rec8; Meiosis; Saccharomyces cerevisiae

Conflicts of Interest

The authors declare no conflicts of interest.

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