Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization

Marlus Chorilli, Hérida Regina Nunes Salgado, Fabiana De Santana Santos, Lucélia Magalhães Da Silva

Departamento de Fármacos e Medicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil.
Faculdade de Americana, Americana, Brazil.
Programa de Pós-gradua??o em Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Araraquara, Universidade Estadual Paulista (UNESP), Araraquara, Brazil.

DOI: 10.4236/ajac.2012.32016                

Abstract

An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.

Keywords

Glutamine; Food Additive Product; Liquid Chromatography

Conflicts of Interest

The authors declare no conflicts of interest.

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