Author(s)

Shunshun Shu, Qian Mi, Caixia Yang, Chonghuan Bao, Zhaoyang Wang, Jun Niu

C.P. Food (Xiangyang) Co. LTD., Xiangyang, China.

A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method; the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.

Heparin Sodium, Dialysis and Spray Drying, Oven Drying Method, The Production Process

Shu, S. , Mi, Q. , Yang, C. , Bao, C. , Wang, Z. and Niu, J. (2018) Heparin Sodium Was Prepared from Pig Intestinal Mucosa by Dialysis and Spray Drying. Journal of Biosciences and Medicines, 6, 5-11. doi: 10.4236/jbm.2018.610002.