Author(s)

C. Leigh Broadhurst^1,2*, Walter F. Schmidt¹, Julie K. Nguyen¹, Jianwei Qin¹, Kuanglin Chao¹, Moon S. Kim¹

¹Environmental Microbiology and Food Safety Laboratory, US Department of Agriculture Agricultural Research Service, Beltsville, MD, USA.
²Department of Mechanical Engineering, University of Maryland Baltimore County, Baltimore, MD, USA.

Specialized pro-resolving mediators provide promising targets for new drugs and natural products. Much work has been accomplished on the structure/ function of the lipoxygenase and cyclooxygenase enzymes but not on the substrates. A better visualization of three-dimensional lipid structures will allow increased refinement of the interactions that produce the pro-resolving mediators, and lead to improvements in synthetic pathways. We present systematic analysis of oleic (18:1n-9), linoleic (18:2n-6), alpha-linolenic (18:3n-3), arachidonic (20:4n-6), docosapentaenoic (22:5n-3), and docosahexaenoic (22:6n-3) acids. Continuous gradient temperature Raman spectroscopy (GTRS) applies the temperature gradients utilized in differential scanning calorimetry to Raman spectroscopy. GTRS can identify and differentiate specific carbon chain sites, finally allowing Raman analysis to explain why the long-chain polyunsaturated fatty acids (LC-PUFA) exhibit such extreme functional differences despite minimal changes in chemical structure. Detailed vibrational analysis of the important frequency ranges 1450 - 1200 cm^-1 (includes CH₂ bending and twisting) and 1750 - 1425 cm^-1 (includes C=C stretching and C-C stretching plus H-C in-plane rocking) shows for the first time that each molecule has its own characteristic set of modes with only some redundancy/commonality. The number and frequency of modes correlates with three-dimensional molecular structure, not the degree of unsaturation. The high degree of specificity of lipoxygenase and cyclooxygenase enzymes should be reconsidered in light of the fact that individual sites on the polyunsaturated fatty acid chain are nonequivalent, and each LC-PUFA molecule has an individual, specific three dimensional structure incorporating torsion.

Gradient Temperature Raman Spectroscopy, Raman Spectroscopy, Arachidonic Acid, Docosapentaenoic Acid, Docosahexaenoic Acid, Alpha-Linolenic Acid, N-3DPA, DHA, GTRS

Broadhurst, C. , Schmidt, W. , Nguyen, J. , Qin, J. , Chao, K. and Kim, M. (2018) Gradient Temperature Raman Spectroscopy of Fatty Acids with One to Six Double Bonds Identifies Specific Carbons and Provides Systematic Three Dimensional Structures. Journal of Biophysical Chemistry, 9, 1-14. doi: 10.4236/jbpc.2018.91001.