Author(s)

Nobuo Yamaguchi^1*, Yoshiichiroh Mastuba¹, Kazuhiro Okamoto², Daisuke Sakamoto³, Takanao Ueyama⁴, Hideo Matsuno⁵, Nurmuhamamt Amat⁶

¹Ishikawa Natural Medicinal Products Research Center, Ishikawa, Japan.
²Department of Rehabilitation, Kanazawa Medical University, Uchinada-Machi, Japan.
³Department of Heart Surgery, Kanazawa Medical University, Himi Municipal Hospital, Himi, Japan.
⁴Department of 2nd Internal Medicine, Kansai Medical University, Osaka, Japan.
⁵Department of Otolaryngology, Kanazawa Medical University, Uchinada-Machi, Japan.
⁶Traditional Uighur Medicine Department, Xinjiang Medical University, Urumqi, China.

Eighty sorts of herbs were fermented by Lactobaccilli (f-ESH). This material was proved by as safe in animal safety experiment. This work was aimed to revise the traditional way of hot water extraction to the fermentation, in order to use up the original material and searching new activity as well. We tried to prove the new activity by fermentation for immune-competent cells in the host that administrated both of original remedy and the new fermented sample. In rodents, immune-compromised host was prepared with cancer chemotherapeutic agent. Our observations showed that the antigen stimulated animal increased the antibody producing cell for heterologous erythrocyte by compromised host by f-ESH. Moreover, by administration of f-ESH to immno-suppressed hosts, these samples regulated lymphocyte in number and functions, macrophage activities, and regulating anti-oxidative activity by phagocytic cells. The anti-oxidative assay was carried out ex-vivo system by peritoneal excaudate cell that we propose as suitable system for evaluating antioxidative assay. With these evidences, the original COF only augmented the level of lymphocytes in number, while f-ESH regulated the ratio of granulocytes and lymphocyte. In clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio was also resulted as neutral in peripheral white blood cells, 1, 2 and 3 weeks after the administration of f-ESH. We discussed the significance and mechanism of cleving complement components Fb by f-ESH in this text. Fb. The new cleavage of complement was directly evident by immune-electrophoretic method. In conclusion, this modification of food materials introduced new style of food intake resulted in the significant regulation such as antibody producing cells and anti-oxidative activity for phagocytic cells comparing traditional processing by hot water extraction.

Wild Herbs, Fermentation, Lactobacilli, Lymphocyte, Granulocyte, Ant-Oxidative Activity, GABA, Complement, Alternative Complement Activation

Yamaguchi, N. , Mastuba, Y. , Okamoto, K. , Sakamoto, D. , Ueyama, T. , Matsuno, H. and Amat, N. (2017) Lactobacilli Enjoyed Fermented Herbs on to the Last Fragment and Regulated Leucocyte Subsets and Anti-Oxidative Activity. Open Journal of Rheumatology and Autoimmune Diseases, 7, 30-45. doi: 10.4236/ojra.2017.71003.