Detection and Characterization of β-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital ()
Affiliation(s)
1The Dr. James J. Rahal, Jr. Division of Infectious Diseases, Department of Medicine, New York-Presbyterian/Queens, Flushing, NY, USA.
1The Dr. James J. Rahal, Jr. Division of Infectious Diseases, Department of Medicine, NewYork-Presbyterian/Queens, Flushing, NY, USA.
2Department of Biological Sciences, SUNY Old Westbury, Old Westbury, New York, NY, USA.
3Department of Pathology, NewYork-Presbyterian/Queens, Flushing, NY, USA.
ABSTRACT
A surveillance study was undertaken to identify
prominent β-lactamase encoding genes
in 131 carbapenem
non-susceptible gram-negative clinical isolates at a New York City community hospital. KPC carbapenemases were
detected in 89% of Enterobacteriaceae as well as additional TEM, SHV, and CTX-M
class A enzymes. OXA-23 and OXA-24 were the prevalent class D carbapenemases
identified in Acinetobacter species.
One OXA-23 in M. morganii and one
OXA-48 in K. pneumoniae were also
identified. Among class C β-lactamases
CMY, ACT/MIR, DHA, and FOX were detected. The in vitro activity of ceftazidime-avibactam by E-test methodology
was tested with minimal inhibitory concentrations (MIC) of ≤3 μg/ml for 97.8%
of all Enterobacteriaceae, MIC50/90 of 16/>256 μg/ml for carbapenem
non-susceptible Acinetobacter, and
3/6 μg/ml for carbapenem non-susceptible Pseudomonas
aeruginosa. Periodic surveillance of isolates to characterize current and emerging β-lactamase genotypes present in local
isolates may help identify outbreak situations, provide assistance to infection
control and antibiotic stewardship programs, and potentially improve patient
outcomes.
Share and Cite:
Urban, C. , Colon-Urban, R. , LaBombardi, V. , Mariano, N. , Prasad, N. , Dhillon, G. , Guralnik, M. and Segal-Maurer, S. (2016) Detection and Characterization of
β-Lactamase Encoding Genes in Carbapenem Non-Susceptible Gram-Negative Bacteria and Susceptibility of Isolates to Ceftazidime-Avibactam at a New York City Community Hospital.
Open Journal of Medical Microbiology,
6, 150-157. doi:
10.4236/ojmm.2016.64020.
Cited by
No relevant information.