Author(s)

Md. Sahab Uddin^1*, Abdullah Al Mamun¹, Mohammed Ashraful Iqbal², Ariful Islam³, Md. Farhad Hossain¹, Sayema Khanum¹, Mamunur Rashid^1,4

¹Department of Pharmacy, Southeast University, Dhaka, Bangladesh.
²Department of Chemistry, Fareast International University, Dhaka, Bangladesh.
³Department of Pharmacy, State University of Bangladesh, Dhaka, Bangladesh.
⁴Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.

Oxidative stress is intensely linked with neurodegenerative disorders, especially Alzheimer’s disease (AD). Searching for medicinal plant with the nootropic activity for controling the development and progression of AD has received extensive consideration. The plant Phyllanthus reticulatus (PR) Poir. is known in Bengali as Panjuli belongs to family Euphorbiaceae. Previous studies have shown the antioxidant, analgesic, anti-inflammatory, etc. activities of this plant. Therefore, the objective of this study was to examine the nootropic effect of ethanolic extracts of Phyllanthus reticulatus (EEPR) on cognitive functions, brain antioxidatant enzymes and acetylcholinesterase activity in aluminium-induced rats of cognitive impairment and oxidative stress. The effects of EEPR fruit (i.e., 100 and 200 mg/kg b.w.) were examined for 30 days and its nootropic effect was determined in aluminium treated Swiss albino male rats by behavioral studies such as Passive Avoidance (PA) test, Rewarded Alternation (RA) test and biochemical studies such as superoxide dismutase (SOD), catalase (CAT), contents of thiobarbituric acid reactive substances (TBARS) and acetylcholinesterase (AChE) activity in rats brain tissue homogenates. In PA test, administration of EEPR fruit (i.e., 100 and 200 mg/kg, b.w.) significantly (P < 0.05, P < 0.01) increased step-through latency (STL) in rats on 30th day with respect to disease control group. The percentage of memory retention (MR) for this test was pointedly (P < 0.05) increased in rats treated with EEPR fruit (i.e., 200 mg/kg b.w.) as compared with disease control group. For RA test, EEPR fruit (i.e., 200 mg/kg b.w.) markedly (P < 0.01) increased the correct responses (CR) in rats on 30th day related to disease control group. In case of this test the percentage of MR was significantly (P < 0.05, P < 0.01) increased in rats treated with EEPR fruit (i.e., 100 and 200 mg/kg b.w.) with respect to disease control group. Administration of EEPR fruit (i.e., 100 and 200 mg/kg b.w.) considerably (P < 0.05, P < 0.01) increased the level of SOD, CAT and expressively (P < 0.05) decreased TBARS level compared to disease control group. Treatment with EEPR fruit (i.e., 100 and 200 mg/kg b.w.) markedly (P < 0.05, P < 0.01) decreased the level of AChE activity to that of disease control group. The present study shows that EEPR fruit has excellent nootropic effect on cognitive performance and brain antioxidant markers in aluminium-induced rats of cognitive impairment and oxidative stress which could be developed in the management of neurodegenerative diseases especially AD.

Nootropic, Phyllanthus reticulatus, Cognitive Functions, Brain Antioxidant Enzymes, Acetylcholinesterase Activity, Alzheimer’s Disease

Uddin, M. , Mamun, A. , Iqbal, M. , Islam, A. , Hossain, M. , Khanum, S. and Rashid, M. (2016) Analyzing Nootropic Effect of Phyllanthus reticulatus Poir. on Cognitive Functions, Brain Antioxidant Enzymes and Acetylcholinesterase Activity against Aluminium-Induced Alzheimer’s Model in Rats: Applicable for Controlling the Risk Factors of Alzheimer’s Disease. Advances in Alzheimer's Disease, 5, 87-102. doi: 10.4236/aad.2016.53007.