Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker - Open Access Library Journal

OALibJ > Vol.3 No.1, January 2016

Volume 3, Issue 1 (January 2016)

ISSN Print: 2333-9705 ISSN Online: 2333-9721

Google-based Impact Factor: 0.73 Citations

Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker ()

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DOI: 10.4236/oalib.1101625 506 Downloads 1,150 Views Citations

Author(s)

Sánchez Perera Luz María^1*, Janet Piloto², Deyanira Canelsota³, Liliam Pelzer⁴, Betty Mancebo¹

Affiliation(s)

¹Departmentof Chemistry, Pharmacology and Toxicology, National Centre for Animal and Plant Health, CENSA, La Habana, Cuba.
²Center of Medicinal Drug Development, CIDEM, Havana, Cuba.
³Institute National of Oncology and Radiography, INOR, Havana, Cuba.
⁴University of San Luis, San Luis, Argentina.

ABSTRACT

Rhizophora mangle L. is a vegetal species with high disponibility in Cuba for guarantying economical factibility by drug development. Preview studies showed promissory pharmacological results by the use of this plant in the treatment of gastroduodenal ulcers. The present work involved another evaluation of the active extract important by the development of a new drug. It was evaluated by intestinal absorption in vitro and in situ models and anti-diarrheic activity in rats. The polyphenolic fractions were separated from the aqueous extract and its cytoprotective activity was studied on gastric ulceration induced by ethanol plus hydrochloric acid in rats. Some toxicological studies were carried out. We made a validation of HPLC method by the analysis of one mark in the tablet development. The aqueous extract showed high absorption, and high anti-diarrheic effect. The low-molecular weight polyphenolic fraction showed the highest level of gastric protection. No toxicological signs were obtained by it. HPLC method evidenced linearity, precision, sensitivity and accuracy.

KEYWORDS

Rhizophora mangle L., Polyphenols, Intestinal Absorption, Genotoxicology, HPLC Analysis

Share and Cite:

Luz María, S. , Piloto, J. , Canelsota, D. , Pelzer, L. and Mancebo, B. (2016) Further Pharmacological Evidence Supporting the Development of an Antiulcerogenic Drug Based on Rhizophora mangle L. Aqueous Extract. HPLC Method Proposed for Determinating a Chemical Marker. Open Access Library Journal, 3, 1-16. doi: 10.4236/oalib.1101625.

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