Author(s)

Kishan Saha¹, Rabindra Kumar Sinha¹, Surajit Basak², Sangram Sinha^1*

¹Department of Botany, Tripura University, Suryamaninagar, India.
²Department of Molecular Biology and Bioinformatics, Tripura University, Suryamaninagar, India.

Molecular fingerprints of four different species of Curcuma, viz., C. amada, C. caesia, C. longa and C. zedoaria, found in Tripura were developed using Inter Simple Sequence Repeats. Twenty ISSR primers generated 116 loci amplified in the range of 200 - 5000 bp with an average of 5.8 alleles and 1.6 effective alleles per locus. The percentage of polymorphic band was found to be 86.29 with an average of 5.15 per primer. Based on UPGMA algorithm these four species are placed in two different clusters that validate the classification based on external and internal morphological characters. The polymorphic ISSR markers generated from this study will be useful for understanding the genetic relationship of different species of the genus Curcuma.

Curcuma, Genetic Diversity, Inter Simple Sequence Repeats, Polymerase Chain Reaction, Taxonomy, Zingiberaceae

Saha, K. , Sinha, R. , Basak, S. and Sinha, S. (2016) ISSR Fingerprinting to Ascertain the Genetic Relationship of Curcuma sp. of Tripura. American Journal of Plant Sciences, 7, 259-266. doi: 10.4236/ajps.2016.72025.