Author(s)

Hasan Bakhshi¹, Mohammad Reza Abai¹, Gholamreza Amin², Rasoul Zolfi¹, Masoumeh Pirmohammadi¹, Azam Bakhshi³, Fakhredin Taghinezhad⁴, Seyed Hasan Moosa-Kazemi^1*

¹Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
²Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
³Department of Nursing, School of Nursing and Midwifery, Qom University of Medical Sciences, Qom, Iran.
⁴School of Nursing and Midwifery, Islamic Azad University, Medical Sciences Branch, Tehran, Iran.

The aim of this study was to determine the larvicidal activity of Lawsonia inermis against Anopheles stephensi as the main malaria vector in Iran. This study was carried out from February to July 2011. Larvicidal activity of >L. inermis was studied in the range of 4 - 4000 PPM in the laboratory against early and late stages of larvae of An. stephensi. The larvae were reared in the insectarium. The LC50 and LC90 values of the larval stages of An. stephensi were calculated by probit analysis and regression line draw using Microsoft office excel 2003 software. The highest toxic effect of L. inermis was found at 4000 PPM and the lowest at 4 PPM against larval stages I and II. The same result was found against larval stages III and IV. The LC50 and LC90 was found as 413.8 and 3366.3 respectively against larval stages I and II while against late stages found as 696.9 and 3927.7 respectively. This study suggests that L. inermis extract can be used as an alternative larvicidal compound during the IPM programs for the An. stephensi control. It is recommended to investigate the competency of other similar plants to malaria control.

Anopheles stephensi, Lawsonia inermis, Larvicidal Properties

Bakhshi, H. , Abai, M. , Amin, G. , Zolfi, R. , Pirmohammadi, M. , Bakhshi, A. , Taghinezhad, F. and Moosa-Kazemi, S. (2014) Larvicidal Properties of Botanical Extracts of Lawsonia inermis against Anopheles stephensi. Advances in Infectious Diseases, 4, 178-185. doi: 10.4236/aid.2014.44025.