American Journal of Plant Sciences
Volume 4, Issue 6 (June 2013)
ISSN Print: 2158-2742 ISSN Online: 2158-2750
Google-based Impact Factor: 1.20 Citations h5-index & Ranking
Improved Micropropagation in Hybanthus enneaspermus L. Muell ()
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ABSTRACT
Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction, different types of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half-strength MS medium supplemented with 2.46 μM·l-1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.
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