Food and Nutrition Sciences

Volume 3, Issue 4 (April 2012)

ISSN Print: 2157-944X   ISSN Online: 2157-9458

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Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity

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DOI: 10.4236/fns.2012.34072    7,904 Downloads   13,668 Views  Citations

ABSTRACT

Several protein sources can be used to produce bioactive peptides with angiotensin I-converting enzyme (ACE) inhibittory activity. Protein concentrates from ungerminated and germinated lima bean Phaseolus lunatus seed flours were hydrolyzed with Alcalase 2.4 L or pepsin-pancreatin sequential hydrolysis, and ACE inhibitory activity measured in the different hydrolysis treatments. Protein hydrolysate production was analyzed with a 23 factorial design with four replicates of the central treatment. Evaluated factors were protein concentrate source (ungerminated seeds, PC1; germinated seeds, PC2), enzyme/substrate ratio E/S (1/50 or 1/10) and hydrolysis time (0.5 or 2.0 h for Alcalase; 1 or 3 h for pepsin-pancreatin). Degree of hydrolysis (DH) was high for the Alcalase hydrolysates (24.12% 58.94%), but the pepsin-pancreatin hydrolysates exhibited the highest ACE inhibitory activity (IC50 = 0.250 0.692 mg/mL). Under the tested conditions, the hydrolysates with the highest ACE inhibitory activity were produced with sequential pepsin-pancreatin using either PC1 at 1 h hydrolysis time and a 1/10 E/S ratio or PC2 at 1 h hydrolysis time and a 1/50 E/S ratio. Lima bean protein hydrolysates prepared with Alcalase or pepsin-pancreatin are a potential ingredient in the production of physiologically functional foods with antihypertensive activity.

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L. Chel-Guerrero, M. Domínguez-Magaña, A. Martínez-Ayala, G. Dávila-Ortiz and D. Betancur-Ancona, "Lima Bean (Phaseolus lunatus) Protein Hydrolysates with ACE-I Inhibitory Activity," Food and Nutrition Sciences, Vol. 3 No. 4, 2012, pp. 511-521. doi: 10.4236/fns.2012.34072.

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