Innovation of Neuropsychopharmacological Experimental Methods to Investigate Attention in Human Participants and Its Validation by Randomized Double Blind Placebo Controlled Clinical Trial

In terms of advancement of life to meet the challenges new method for neuropsychopharmacological research are continually developing to ensure superior safety, accuracy, efficacy, precision etc. In this experiment, we suggested and validated two neuropsychopharmacological testing methods, these are Numeral Finding (NF) and Typo Revealing (TR) tests for the determination of attention in healthy aged human participants. The objective of this experiment was to generate more effective testing methods for the assessment of attention in human. The rationality of the aforementioned methods was checked by a randomized double blind placebo controlled clinical trial for the period of 21 days among healthy aged human participants. The experiment was conducted at Gazipur Sadar, Gazipur-1703, Dhaka, Bangladesh between the period of December 2015 and March 2016. The experiment center was the Department of Pharmacy, Southeast University, Dhaka-1213, Bangladesh. In this experiment, 60 healthy male participants between the age ranges of 40 to 50 years old were selected and randomly divided by simple randomization process into 3 groups with 20 participants in each. Ginkgo biloba standardized extract’s capsule (Giloba® and Kobi®) was used as a standard and as a placebo control psyllium seed husk (i.e., seed of the Plantago ovata) was used after filling in the hard gelatin capsule shell (size 0). Group 1 was treated with Giloba® in which each capsule contained 120 mg Ginkgo biloba standardized extract (GBG) and group 2 was treated with Kobi® in which each capsule contained 60 mg Ginkgo biloba standardized extract (GBK). The treatment procedure for group 3 was psyllium seed husk’s capsule that served as placebo control (PC). Throughout the experiment each capsule was administered 3 times daily. Corresponding author.


Introduction
The human brain is the main organ of the human central nervous system (CNS) that regulates all functions.The extraordinary human abilities depend on several factors in which attention is vital [1].Attention is the process of concentrating on a specific aspect of information to accomplish the chosen task by filtering distracting information [2].The attention power of human varies from man to man.Every discovery and invention is the results of extensive attention of not only complex, but also simplex things present in our surroundings [3].Several studies have exposed that in Einstein's brain regions involved with numerical and spatial processing are larger than regions involved in speech and language.Further studies have suggested an increased number of glial cells in Einstein's brain [4].The performance of any work depends on attention.The expression of attention may vary in strength from a low intensity to a high intensity as well as may vary in duration short to sustain [5].
Attention is the precursor to all other neurological/cognitive functions [6].In the course of attention multiple stimuli from the outside environment bombard body's specialized sensory receptors and the brain performed searching to select only desire subject of interest [7].During searching brain frequently shifts its focus between external events and internal memories and interests.In order to maintain and upgrade memory, this sifting of internal and external events is mandatory [8].Research suggests that brain cell cannot process all stimuli, it only processes those stimuli that are most potent.In addition to this, automatically brain cell focuses on that information that contains high contrast and or emotional intensity [9].
Attention deficit hyperactivity disorder (ADHD) is a neurobehavioral disorder involving behavior dysfunctions and cognitive impairments.ADHD is characterized by persistent and chronic inattention and or hyperactivity-impulsivity that interfere with the functioning or development of the brain [10].This disease is most common among in childhood and can continue through adolescence and adulthood.Worldwide the prevalence of ADHD is about 7% and boys are four times at risk than girls [11] [12].Attentional deficits are frequently seen in isolation as the presenting sign and symptom of neurodegenerative disease, manifest as mild cognitive impairment (MCI) [13].For geriatric people persistent ADHD leads to neurodegenerative diseases.In fact, alteration of neuroanatomic assemblies or neurochemical function and or pathway is the causative agent for the development and propagation of de novo late life neurodegenerative disease such as Alzheimer's disease (AD), Parkinson's disease (PD), Lewy body dementia (LBD), frontotemporal dementia (FTD) etc. [14].
Neuropsychological tests are linked to the brain structures or pathway into brain functions [15].In order to measure memory, intelligence, attention, cognitive, visuospatial, executive function etc. in patients with neurological disorders or psychological disorders or neuropsychological disorders, various types of neuropsychological tests is effective [16].In addition to this, in the research of human brain and diagnosis of neuropsychological disorders, they are widely used [17].But currently used most neuropsychological tests are based on traditional psychometric theory [18].Therefore, the intention of this experiment is to display and verify two neuropsychopharmacological testing methods these are Numeral Finding (NF) and Typo Revealing (TR) tests to resolve attention in normal aged human participants.

Objectives
The specific objectives of NF and TR tests are offered below: 1. To measure attention deficits in patients with neurological and or psychological or neuropsychological disorders NF test is efficient.
2. To determine attention problems in patients with neurological and or psychological or neuropsychological disorders TR test is operative.
3. To confirm the attention enhancing capacity of unknown medicinal plants/drugs in human NF and TR tests is efficient.
4. To check the attention power of human NF and TR tests is competent.5. To endorse the presence or absence of attention deficits of patient's with neurological and or psychological or neuropsychological disorders NF and TR tests is impressive.
6. To confirm the effect of established drugs in the attention of human NF and TR tests is effective.

Study Design
The study protocol of the randomized double blind placebo controlled clinical trial of this experiment is specified in Figure 1.Simple randomization process was used to randomize the participants.The place of the experiment was Gazipur Sadar, Gazipur-1703, Dhaka, Bangladesh.The experiment center of this clinical trial was the Department of Pharmacy, Southeast University, Dhaka-1213, Bangladesh.The protocol of the experiment was approved by the ethics committee of the Department of Pharmacy, Southeast University, Dhaka-1213, Bangladesh.

Study Population
In this experiment healthy 60 male participants between the ages of 40 to 50 years old were arbitrarily selected and till last of the experiment all the participants attended.Table 1 represents the demographic information of the participants.Since it is not possible as well as unethical to create attention deficits in human to validate the proposed methods, as a consequence healthy aged participants were chosen.In addition to this, it is not likely to choose participants with attention problems (i.e., ADHD, AD), but able to partake in this experiment.Oxidative stress plays a major role in the aging process and intensely related to it.Many studies have suggested that increased oxidative stress has been implicated in the genesis and propagation of many disorders like neurological disorders, neurodegenerative disease, dementia, cognitive impairment etc.In fact aged people are more susceptible to oxidative stress than younger one and confirmed by several studies [19]- [21].Before selection of the participants they were subjected to a comprehensive health related questions to estimate the health status.Prior to the experiment, from each participant written informed consent was taken.

Inclusion and Exclusion Criteria
In this experiment before selection of the participants following conditions were considered: 1. Devoid of any neurological disorders at previous times.
2. Devoid of any psychological disorders at preceding times.
3. Devoid of drug addiction.4. Devoid of any severe illness within the earlier 3 months.5. Deprived of cardiovascular, renal, hepatic, eye disease and diabetes.6. Participants who miss any doses for more than a day, were intended to vanish from the experiment.Furthermore, prior to selection a number of factors, including lifestyle, socioeconomic condition, personal issues, work profile, work stress, food habits, medication history, intelligence etc. of the participants were inspected.The educational background and body surface area of the participants were also considered during selection of the participants.

Standard Drugs
Ginkgo biloba is a well-known established natural nootropic herbal drug [22].In this experiment Ginkgo biloba standardized extract was used as a standard drug to examine the efficacy of the proposed testing methods.Ginkgo biloba leaf extracts contain several active constituents including flavonoids, terpenoids and organic acids.The majority of Ginkgo biloba leaf extract is standardized to contain 24% flavone glycosides (primarily com- where, SSC = Secondary school certificate, HSC = Higher secondary certificate, Degree (Pass) = This is considered to be a pass but without the honours.

Treatment
In this experiment as standard drugs Ginkgo biloba standardized extracts (Giloba ® and Kobi ® ) were administered to participants for 21 days to validate the efficacy of the proposed testing methods.The dosage form of the Giloba ® is capsule and each capsule contains 120 mg Ginkgo biloba standardized extract.On the other hand, Kobi ® is also available as capsule that contains 60 mg Ginkgo biloba standardized extract per capsule.Psyllium seed husk (i.e., seed of the Plantago ovata) was filled in the empty hard gelatin capsule shell (size 0) to prepare the capsule and used as placebo control (PC).The manufacturing process of the capsules was completed in the ISO and GMP certified Modern Pharmaceuticals Ltd., Dhaka, Bangladesh.Psyllium seed husk has no nootropic activity and non-toxic that's why it was chosen as placebo control.After consultation with physician and literature searches the doses of these standard drugs and duration of the experiment were adjusted [23] [24] [28] [29].Allopathic drugs were avoided in this experiment owing to the risk of excessive cholinergic activity as well as adverse effects in the participants since they were healthy aged.In addition to this, a number of studies of herbal drugs suggested that they are safer than allopathic drugs [30]- [33].The treatment period of this clinical trial was 21 days.

Experimental Design
The participants were randomized into three groups with 20 participants in each as follows: Group 1: This group received Ginkgo biloba standardized extract (120 mg/capsule), 1 capsule 3 times daily for 21 days (GBG).
Group 3: This group received psyllium seed husk (hard gelatin capsule shell size 0) as placebo control, 1 capsule 3 times daily for 21 days (PC).
The participants were suggested to take capsule before 30 minutes of meal with plenty of water for better absorption.The chance of underdose and overdose owing to the sub-thepareutic and excessive concentration of the drugs were removed by recommended them to take capsule after each 8 hour interval for 3 times daily.Throughout the experiment normal diet was proposed to take and health status of the participants was checked after each 3 day's intermission with the help of the physician.All participants were instructed to call the hotline of the experiment if they experienced any adverse effects during and after the experiment.To remove the chance of post treatment complications, the participants were kept under physician checkup for 7 days after the experiment.
In this experiment NF and TR tests were conducted among participants between the period from 10:00 am to 12:00 pm on 7 th , 14 th and 21 st day.

Numeral Finding Test
This test is based on finding the wanted numerals among a set of numbers.In this test 100 numbers are placed randomly in a chart that consists of ten columns and ten rows in a printed paper and given to the subject offered in Figure 2. The numbers must be consisted of double digit (i.e., 10 to 99).From 1 to 100 if single digits (i.e., 1 to 9) and triple digit (i.e., 100) are not considered there are 90 double digits.When these double digits are placed unsystematically in the chart to make 100 numbers, 10 numerals must be added that will serve as wanted numeral (Figure 3).These wanted numerals must be the repetition of at least three numbers.In Figure 3, numeral 14, 50  where, TNCWNs = Total number of correct wanted numerals identified by the subject, TNPWNs = Total number of presented wanted numerals in the chart.An increase in IA is considered as an index of upgraded attention.

Typo Revealing Test
This test is based on detecting the typo from a passage.In this test a standard passage contains 250 words is given to the subject to read carefully at a glance presented in Figure 4.After that intentionally some typological mistakes are incorporated to the passage.This type of mistake may addition of any letter/punctuation (i.e., neu- where, TNCTs = Total number of correct typos identified by the subject, TNPTs = Total number of presented typos in the passage.An increase in IA is considered as an index of greater attention.

Statistical Analysis
All the results were expressed as mean ± SEM and analyzed statistically by one-way analysis of variance (ANOVA).Student's t-test was used for the comparison of PC group and various groups.All statistical and graphical analyses were accomplished using SPSS 16.0 (Chicago, IL, USA) and MS Excel 2010 (Roselle, IL, USA).The value of P < 0.05 was considered as statistically significant with respect to PC group.

Efficacy of the NF Test
NF test was used to examine the attention based on the finding of wanted numeral.Figure 6 shows the results of NF test measured on 7 th , 14 th and 21 st day.Treatment with GBG and GBK standardized extracts for successive  days gradually increased the attention of the participants with regard to PC group.The administration of GBG extract significantly (P < 0.05, P < 0.01) increased the attention of the participants on 14 th and 21 st day as compared to PC group.Particularly, GBK extract administration markedly (P < 0.01) increased the attention of the participant's on 14 th and 21 st day related to PC group.

Efficacy of the TR Test
The typological error detecting ability of the participants was measured on 7 th , 14 th and 21 st day displayed in Figure 7. Treatment with GBG and GBK standardized extracts gradually increased the attention of the participants in comparison with PC group.GBG extract treated participants exhibited significantly (P < 0.01) increased in attention on 14 th and 21 st day with respect to PC group.Specifically, administration of GBK extract prominently (P < 0.001, P < 0.01) increased the attention of the participants on 14 th and 21 st day as compared to that of PC group.

Discussion
The brain is the control center of the human body [34].Understanding the relationship of brain learning, attention and consciousness is an interesting area of research for neuroscientists.Attention is recognized as one of the three major co-active processes of the working brain [35].In this experiment we proposed and validated NF and TR tests for the determination of attention in aged human participants.
The brain has many diverse regions, but in case of functioning it operates in a remarkable integrated way [36].In the attention process brainstem, neurotransmitter, limbic system and neocortex play an important role [37].The brainstem passively receives incoming sensory information and starts the process of active attention [38].In the control of arousal and to ignore irrelevant stimuli, reticular formation and locus coeruleus are accountable [39].Among neurotransmitter, norepinephrine and dopamine are the important neurotransmitter appear to control the processing of attention [40].Studies suggested that the level of neurotransmitter fluctuations in 90 minute cycles across the 24 hours in the body.In the morning the level of neurotransmitter is higher as a re- sult, many people experience a sharp rise in the attention that causes to wake up.During the afternoon the level of neurotransmitters begins to decline and after midnight reaches the lowest levels as a result sleep becomes unavoidable [41].Optimum level of neurotransmitter is best for proper functioning of attention [40].The limbic system controls the emotional overtones and motivation for attention [43].The neocortex is the part of the cerebral cortex that plays a key role in sensory perception, generation of motor commands, spatial reasoning, conscious thought, memory and learning processes etc [44].
To increase the attention span conceptual development is effective by merging related elements into a single unit [45].Automatically when we see a face we consider it as a single unit, not as individual eye/ear/nose/mouth.But readers, who primarily focus on individual letters and words, have to learn to read entire phrases as single units [46].In this experiment, we proposed and confirmed NF test for determination of attention that is based on finding wanted numbers among 100 numbers.So in this test there are 100 single units consist of two digits.Among 100 numbers (i.e., 10 -99), finding of wanted numbers that are repeated several times is tough and in this case more concentration is obligatory for finding and calculation of the each repeating number.That's why NF test is more effective than Trail making test [47] in which the subject is instructed to draw lines sequentially connecting 25 encircled numbers/letters randomly spread in a paper.In addition to this, for NF test with attention concentration is prerequisite owing to the presence of point of focus but for Trail making test only minimal attention is necessary.
The principal attentional activity of human being is the constant conscious selection of a current focus [48].In case of attention factors like focus and intensity can differ broadly [49].It will be clarified by comparing between a proofreader and a cursory reader of a magazine article.Initially they cautiously focus to scrutinize individual words and punctuation, then concentrates on the general content.Attention system leads itself in anticipation during try to find such specific information [50].It increases the response levels of the networks that process that information and it inhibits other networks.That's why, the proofreader scrutinizes a page and marks spelling mistakes, while the cursory reader skims the identical page and marks main content words and phrases [51].TR test is suggested and validated for the measurement of attention that is based on the finding of typological errors present in the given passage.When readers read the passage to find typos their attention might divide into the time and typos therefore stronger attention and concentration is vital for finding typos.But for Letter Cancellation test the subject is informed to cross out all the target letters among randomly distributed letters in a paper [52].Consequently, this test is devoid of concentration only minimal attention is required, owing to the aforementioned lacking TR test is superior than Letter Cancellation test.
Fortunately, our attentional system provides us with a short-term memory buffer that allows us to hold a few units of information for several minutes while we determine whether to go on to something else or store the data in our long-term memory.Multitasking is the attempt to execute two or more tasks concurrently.However, the study shows that during multitasking, people make more mistakes and also perform the tasks more slowly [53].On behalf of the proposed and validated testing methods of this experiment, attention must be divided among all of the component tasks to perform.Consequently, NF and TR tests might effectively detect attention power of the human.

Conclusion
The brain is the chair of all intellectual abilities.Attention is the brain-based work supported by specific neuronal networks with the help of neurochemicals.In this experiment, we have developed NF and TR tests for the determination of attention in human and validated the effectiveness of the methods by using a randomized double blind placebo controlled clinical trial.The results of this experiment suggest that NF and TR tests are highly efficacious for the measurement of the attention enhancing power of the both Ginkgo biloba standardized extracts in healthy aged human participants.So these testing methods will be effective for various neuropsychopharmacological assessments in human.The notable feature of these aforementioned testing methods is that these can be performed based on the background of the subject, they are not rigid.Therefore, NF and TR tests will make breakthrough in the compass of neuroscience.

Limitations
Although it was a pilot study the results were statistically significant to bring the impact.However, it would be better if we could perform this experiment among more participants.The nature of participants may also influence the result of the experiment.

Figure 1 .
Figure 1.Blueprint presenting the movement of the participants through the clinical trial.

Figure 2 .
Figure 2. Numeral finding test by using set of numerals.Randomly placed numerals in a chart on the printed paper given to the subject to find wanted numerals.

Figure 3 .
Figure 3. Numeral finding test by using set of numerals.Wanted numerals from the set of numerals [i.e., Figure 2] are marked by red circles.and 62 is repeated 3, 3 and 4 times respectively to fill 10 wanted numerals.Since these numerals fall between 10 to 99 finally numeral 14, 50 and 62 is repeated 4, 4 and 5 times respectively.So lastly, there are 13 wanted numerals consist of repetition of 3 numerals.The time taken by subject, to find the wanted number is considered as numeral finding time (NFT).The duration of this test is 180 seconds.The background of the sample might influence the duration of this test.So to select the duration one can perform this test to a number of samples and choose the mean time.Gradual decrease in NFT indicates progress of the attention.The percentage of improved attention (IA) is calculated by using the formula given below: % of IA TNCWNs 100 TNPWNs = ×

Figure 4 . 5 .
Figure 4. Typo revealing test by using a passage.Standard passage given to the subject to read carefully at a glance.rooninstead of neuron; bra!in instead of brain), deletion of any letter/punctuation (i.e., nerv instead of nerve; Alzheimers disease instead of Alzheimer's disease), substitution of any letter/punctuation (i.e., antiblotics instead of antibiotics; N_acetyl-p-aminophenol instead of N-acetyl-p-aminophenol), inversion of any letter (i.e., sceince instead of science), providing capital letter instead of small letter etc. and given to subject to find the typos existing in Figure5.The time taken by subject, to find the typo is considered as typo finding time (TFT).The duration of this test is 180 seconds.The duration of this test mainly depends on the characters of the sample and the type of the passage used.Consequently, one can select the duration by providing passage to a number of samples to read carefully and select the mean time.Gradual decrease in TFT indicates improvement of the attention.The percentage of improved attention (IA) is calculated by using the formula given below: % of IA TNCTs 100 TNPTs = ×

Figure 5 .
Figure 5. Typo revealing test by using a passage.Typological mistakes from the standard passage [i.e., Figure 4] are marked by red circles.

Figure 6 .
Figure 6.Effect of Ginkgo biloba standardized extract on attention of human participants using NF test.Values were expressed as mean ± SEM (n = 20/group).GBG = Ginkgo biloba standardized extract of Giloba ® , GBK = Ginkgo biloba standardized extract of Kobi ® , PC = Placebo control.* P < 0.05, ** P < 0.01 significant difference from the PC group.

Table 1 .
Demographic information of the participants.