Disinfection Water Wells and Sterilization

Disinfection is an important step in ensuring that water is safe to drink. Well disinfection is used to inactivate or control bacteria populations in a well and the distribution system. Disinfection is the inactivation or destruction of pathogenic organisms through the disruption of the organisms’ normal life processes. The objective of this work is to clean and/or disinfect entire borehole to prepare wells for pump installation. The last water sample was collected after the well disinfection on that time. The results of water chemical analysis are presented in Tables 2-4, and there was nothing found against the specification requirements. After a reaction time of approximately 12 h, the well was cleaned from the sterilization solution by airlifting until chlorine concentration in the water reached 0 mg/l. Then through analyzing the test for samples of water after disinfection and sterilization the wells showed the Iron Bacteria were not seen and the Bacteria Species/Anaerobic Bacteria were absent.


Introduction
The source of the water determines the characteristics of the water.Water sources, in order of decreasing quality, include springs, boreholes, sealed wells, hand-dug wells, streams, rivers, and lakes.Boreholes are wells drilled with a drilling rig, and like springs, tap groundwater sources that have been filtered through layers of soil and rock and are isolated from the surface.These sources may contain unpleasant color, odor or minerals but are generally free from pathogen contamination and, therefore, will not require disinfection.Sealed wells are shallow wells that have been sealed with cement around a pump to prevent contamination.However, contamination is possible, and sealed wells are often treated with chlorine.Hand-dug wells are typically contaminated.Wells become contaminated from contamination water entering the well from above, especially during flooding.Improper drainage (sloping in toward the well) also promotes well contamination.
In a paper published in 1894, Moritz Traube formally proposed the addition of chlorined of lime (calcium hypochlorite) to water to render it "germ-Free".Two other investigators confirmed Traubes findings and published their papers in 1895 [1].Chlorine in the form of a mixture of chloride of lime and chloride of iron were added to a highly colored water supply over the period of 1902 to 1921 [2] [3].
In the USA, the first use of chlorine for continuous disinfection of a drinking water supply took place in 1908 at Boonton Reservoir (on the Rock way River), which served as the supply for Jersey City, New Jersey [4].
Chlorine gas was first used on a continuing basis to disinfect the water supply at the Belmont filter plant, Philadelphia, Pennsylvania in 1913.Darnell's work and the technological innovations by Dr. George Ornstein and the Wallace & Tiernan Company became the basis for present systems of chlorination of municipal water supplies.By 1941, disinfection of U.S. drinking water by chlorine gas had largely replaced the use of chloride of lime [3] [5].When chlorine is added to water, it reacts to form PH dependent equilibrium mixture of chlorine, hypochlorous acid and hydrochloride acid [6] 2 2

CL H O HOCL HCL +  → +
Depending on the PH, hypochlorous acid partly dissociates to hydrogen and hypochlorite ions:

 → +
In acid solution, the major species are CL 2 and HOCL while in alkaline solution effectively only CLO − is present.Very small concentrations of 2 CLO − , 3 CLO − , 4 CLO − , are also found [7].Natural ground water from all but very shallow aquifers is considered free from pathogenic (disease causing) bacteria and viruses (Bouwer, 1984) [8].As such, ground water obtained from properly designed and constructed water wells is generally free of diseases causing bacteria, and continuous disinfection is unnecessary (Roscoe Moss company, 1990) [9].However, disinfection of a new or repaired water supply system is needed to remove contaminants introduced during the construction or repair process (Driscoll, 1986).Existing water supplies require disinfection when routine maintenance of the system takes place, or when the results of water samples show the presence of coliform bacteria (Driscoll, 1986) [10].
Disinfection is the inactivation or destruction of pathogenic organisms through the disruption of the organisms' normal life processes (Connell, 1996) [11].Disinfection does not mean sterilization result of the effective disinfection is production of potable or drinkable water.
The objective of this study includes the proper water supply system preparation, including the sanitary completion of water well, the cleaning of existing wells, and the proper development of new wells, through flushing of the water supply, by treatment with a properly prepared chlorine solution, collection and analysis of water samples.

Sanitary Drilling Practices
The introduction of microbial contaminants, into newly constructed or repaired wells can be minimized by practicing good general sanitation during storage, transport, handling and installation of well components.
Proper sanitation refers to cleanliness and taking precaution to prevent disease by not introducing bacteria and other contaminants while installing or repairing a water supply system.When the drilling contractor takes precautions to prevent the unnecessary introduction of contaminants into the well, the task of disinfecting the well becomes much easier.
The storage and transportation of well components and drilling supplies should be in good sanitation by the contractor starts at the shop and is important during the trip to the drilling site.The organization and storage of materials and equipment will benefit the contractor during the installation and final disinfection of the completed water supply system [12].

Well Evaluation
Prior to install the permanent pump CCTV survey was done to check the borehole conditions.The first CCTV survey (as final check) was carried out after the well A was completed from the depth of 144 m to the end of PHC, some areas were seen with minor biofouling, including the stripped pattern as shown (Figure 1).Through the final CCTV and bacteriological laboratory results on the collected water samples it was confirmed that in the well B, ongoing processes from biofouling and corrosion may not affect the future productivity or integrity of the well.

Factors Influencing Chlorination
1) Constituents of water-Natural waters represent complex solutions of many substances, most of which can be ignored, but some of which influence chlorination as follows: a) Suspended solids: may shield bacteria from the faction of chlorine.b) Organic Matter: reacts with chlorine and removes it from the water so that it no longer has disinfecting properties.
c) Ammonia-reacts with chlorine to form a chlorine compound (chloramines) having much lower disinfecting qualities than chlorine itself.d) Nitrates, Manganese and Iron-react with and remove chlorine and may produce a false color when testing water for chlorine content.
2) Temperature-The temperature of water marked by affects the disinfecting action of residual available chlorine.Other things being equal, chlorination is most effective with high water temperatures.At lower temperatures, bacterial kill tends to be slower and higher residual concentrations are needed.The effect of low temperatures is greater with combined available chlorine than with free available chlorine, 1890, 1978.
3) PH-The PH of the water affects the disinfecting action of chlorine, particularly combined available chlorine residual that the disinfecting action of chlorine is more efficient at lower PH values of the normal PH range of water supplies.
4) Time-concentration, these two mutually related factors take into consideration the period of reaction time available for disinfection and the quantity and Kind of chlorine residual.A greater concentration acting over a longer period of time must be provided, on the other hand, if free available chlorine residual, an active disinfec-tant, is to be maintained the reaction period can be correspondingly less.The minimum contact time should be 30 minute and preferably with an undesirable concentration of residual chlorine.
e) Chlorine Dosage The amount of chlorine needed to accomplish disinfection or other treatment objectives, depends on the chlorine demand of the water, the amount and kind of chlorine residual desired, the time of contact of the chlorine with the water and the volume of flow to be treated [13].Chlorine demand depends on the kind and concentration of chlorine reactive materials in the water, temperature and contact time.The chlorine required is that amount necessary to affect a specific chlorine residual at the end of a fixed contact period, the combination of which, under well defined operating conditions (PH and temperature) is demonstrably effective in accomplishing disinfection as well as providing the desired free chlorine residual at the end of specific contact time.The required dosage (rate of chlorine application per unit of water flow) to disinfect water supplies not subject to significant contamination normally should not exceed 2 mg/L.The required chlorine feed rate is obtained as follows: Chlorine feed rate = chlorine Dosage (mg/L) * flow (8.34) lb/gal Pounds per day Percentage of free chlorine available 1 mg/L = 8.34 lb of free chlorine per million gallons 1 mg/L = LPPM.

Dosage and Calculations
1) Stock solution: the quantity of hypochlorite solution prepared at one time for the stock solution should be sufficient to last for about two weeks.A common strength for the solution to be fed into the system is 1% available chlorine; this means that every gallon, or 8.34 lb., will contain 0.0834 lb., or 1.34 oz., of active chlorine.If 70% hypochlorite is used, each gallon of 1% solution will contain 1.34/0.7,or 1.92 oz. of material.The weights in ounces of hypochlorite required to chlorinate various quantities of water from a 1 mg/L to a 200 mg/L dosage.The gallons of water needed to prepare a 1% solution using this quantity of hypochlorite, is found by divided the weight in ounces by 1.92 in the case of 70% hypochlorite.
2) Feed Rate: The rate at which stock solution is fed into the system should be based on the actual pump age, in gallon per minutes, and not on the average over-all 24 hour consumption.The volume of 1% chlorine solution needed per minute to furnish a 1 mg/L chlorine dosage when injected into a pipe line following 1000 gpm is:

Well Disinfection
After constructing or repairing a well or pump, the entire well and pumping system must be disinfected in order to kill harmful microorganisms (germs and bacteria) that may be on the well casing, gravel, soil, rising main, pumping rod or in the water from the digging operation.A life water well was just drilled to a depth of 500 to 550 meters (1640.5 to 1804.5 fetes) [14].The pumping rod and riser pipe have been installed and, prior to sealing the well, it should be disinfected.The water level in the well is 126.9  Calcium Hypochlorite comes in strengths ranging from 30 -75 percent available chlorine 70 percent most common).Chlorine is a very reactive substance.When added to a well, it first combines with inorganic compounds (hydrogen sulphide, ferrous iron, manganese) there no disinfection in this stage.After these compounds have been reduced, remaining chlorine then reacts with organic matter (algae, phenols and slime growth).After the demand exerted by inorganic and organic compounds has been met, chlorine will combine with nitrogen compounds (Primarily ammonia) to form chloramines.This combined chlorine form results in long lasting disinfection.
During the contact time the chlorine residual should be maintained in the water supply system for 4 to 12 hours (Coombs, 2001) [15].For increased contact time to be most effective, the pH of the chlorine solution in the well must be maintained between 6 and 7 to keep the chlorine in anon oxidative state (hypochlorous acid).If pH control is not going to be used during periods of increased contact time, use concentrations of chlorine 50 ppm or less (Schnieders, 1998(Schnieders, , 2001) ) [16] [17].
Table 1 shows the sterilization of the well was carried out by injecting 100 ppm Ca-Hypochlorite solution through the drill pipes.For this purpose 4.5 kg of Ca-Hypochlorite were mixed with 56 m 3 of water and pumped through the drill pipes and the washing tool at eight different depths between 209 m bRP and 533 m bRP.At each depth, 8 m³ of solution was injected.After a reaction time of approximately 12 hr, it was confirmed with to Coombs, 2001 for increased contact time to be most effective, the well was cleaned from the sterilization solution by airlifting until chlorine concentration in the water reached 0 mg/l.produce minimal chlorine taste/odor and control organic growths.
From on site measurements, however, chlorine has also found application for a variety of other water treatment objectives, such as, the control of nuisance organisms, oxidation of taste and odor compounds, oxidation of Iron and manganese, color, removal, and as a general treatment aid to filtration and sedimentation processes (Connell, 1996;Williams and Culp, 1986) [11] [18].Proper development of the well facilitates removal of these materials, which increase the success rate the disinfection stage well water must be free of turbidity.Suspended particles in turbid water interfere with the chlorines efforts to make contact with a kill microorganisms (Lechevallier et al.) [19].

Water Quality Field Hydrochemistry and Water Sampling
Water samples for chemical analyses were collected from the sampling tap during SRPT.The samples were taken 1 hour and 23 hours after the start of the test.Both samples were analyzed for physical parameters, major and minor ions.The second sample was, in addition, analyzed for bacteriological parameters.The two samples were received by the Laboratories and Water Quality Sector, Water Authority of Jordan, Amman.On-site measurements are presented in Table 2.
A third sample was collected after well sterilization and analyzed (Table 3, Table 4).

Results Interpretation
Laboratory Analyses of the Jordanian Drinking Water Standards are based on standards from the World Health Organization.As the Jordanian standards do not mention threshold values for certain parameters, in such a case the values stipulated in the EU guidelines from 1998 are used as reference [11].
The results of the laboratory analyses of the three samples taken are presented in Table 2, which shows a comparison of the laboratory results with the respective threshold values of aforementioned standards: The total dissolved solids 320 mg/l are low and typical of low mineralized groundwater from sandstone aquifer.These values correspond to the electrical conductivity, with values between 370 and 339 μS/cm.The threshold value for manganese (0.1 mg/l) is exceeded on both samples, with concentrations of 0.27 and (0.48 mg/l) aerobic.
The bacteriological analyses after 23 hr of steady rate pumping show the presence of aerobic 1981).The turbidity is normally residuals of drill cuttings and/or drilling fluids that have been pushed out into the formation surrounding the borehole during the well construction process.Turbidity levels for common water sources are given in Table 2.
The turbidity of well water is quite low, Sand content, turbidity, pH, temperature and EC were measured during airlift discharge).The acceptance criteria were met for each discharge rate: i.e.Turbidity < 0.2 NTU and sand content 0 ppm, after completing airlift pumping and backwashing, simultaneous swabbing/airlifting, until the acceptance criteria were met EC < 383 μS/cm and sand content 0 ppm.These results illustrated in Figure 2(a), Figure 2(b) the well was cleaned from the sterilization solution by airlifting until chlorine concentration in the water reached 0 mg/l (ppm).While the turbidity of dirty river and lake may be several orders of magnitude greater.As a result of population pressure, increased development, and decreasing costs of pumping water from wells, the aquifer resources are declining in many parts of the world.
It can be expected that future use of the more turbid surface water will increase relative to well water usage (Feachem, Mc Garry, and Mara, 1977) [20].The turbidity levels cause filters to become clogged rapidly, thereby increasing the filter maintenance needs.Sedimentation basin allows much larger particles to settle to 25 micrometers, eliminating most of turbidity and also remove organic and ionic color.When treating surface water pretreatment to remove turbidity must be include in the cost of chemical, UV, and filtration system (Schulz, Okun, 1984;Cheremisin, 1995) [21] [22].
On site measurements it was obtained the following results after sterilization which illustrated in Table 3.
From Table 4 Water from a water supply system that has been treated with chlorine can be turbid due to the effect of the chlorine on minerals in the water, the analyses test for samples of water after disinfection and sterilization the wells show the Iron Bacteria were not seen and the Bacteria Species/Anaerobic Bacteria was absent.Extended pumping normally clears the water of turbidity, and shown the turbidity of well water after sterilization is quite low 0.21, and Hypochlorite acid (HOCL) will form in waters ranging from PH 6.5 -7.79.As the PH increase above 7.5 affects disinfection speed with high temperature provide to fast disinfection.Nitrate concentrations above 10 mg/l can cause blood disorder in infants (blue baby disease).Elevated levels indicate that mature, sewage, or nitrogen fertilizers are reaching the source.If nitrate levels above 45 mg/l, (10 mg/l nitrates as nitrogen) persist, the source of nitrate animal confinement areas, privies should be determined and relocated.The nitrate concentration is above 1 mg/l the water must not be given to infants and a different source (boiled for disinfection).Table 4 shows that the concentration of nitrate range between 9.6 mg/l (boiled for disinfection) must be used.Many naturally occurring minerals and chemicals such as Calcium, Potassium, Sodium or Fluoride are beneficial to human health.These will impart a taste to the water that may take some getting used to.The total dissolved solids range 260 mg/l and are typical of low mineralized groundwater from sandstone aquifer.These values which correspond to the electrical conductivity with values 383 μS/cm are low.

Conclusion
The total dissolved solids 320 mg/l are low and typical of low mineralized groundwater from sandstone aquifer.These values correspond to the electrical conductivity, with values between 370 and 339 μS/cm.The threshold value for manganese (0.1 mg/l) is exceeded on both samples, with concentrations of 0.27 and 0.48 mg/l aerobic.The turbidity of well water is quite low, sand content, turbidity, pH, temperature and EC were measured during airlift discharge.The acceptance criteria were met for each discharge rate: i.e.Turbidity < 0.2 NTU and sand content 0 ppm.The analyses test for samples of water after disinfection and sterilization the wells showed the Iron Bacteria were not seen and the Bacteria Species/Anaerobic Bacteria was absent.

Figure 1 .
Figure 1.CCTV comparison well cleaning before and after disinfection.

Figure 2 .
Figure 2. (a) Sand content and Electrical Conductivity measurements during Swabbing and Airlifting.(b) Sand content and turbidity measurements during High Rate Pumping and Backwashing.

Table 2 .
On-site physicochemical parameters measurements during SRPT.

Table 3 .
On site measurements.

Table 4 .
Analysis test report for sample of water after disinfection and sterilization the well.