Gene Action Studies in the Inheritance of Economic Traits in Diallel Cross of Lentil ( Lens culinaris Medik )

The present study was conducted in 4 × 4 half diallel cross fashion using the genotypes named as NL-2002, NL-2006, Punjab Masoor-2009 and Markaz-2009 for the evaluation of gene action in the inheritance of economic traits of lentil by following the Griffing (1956) Method of analysis. Minirap 13.1 Copyright© 2000, Minitab inc. Method II, Model I was used for the analysis and gene action was computed by variance component analysis using Mixed Model with GLM (general linear model) procedure. The F1 direct crosses along with the parental genotypes were then evaluated by framing the experiment in RCBD in 3 replications. The data were recorded for the characters which are under the study are, plant height (cm), number of primary branches, number of secondary branches, number of pods per plant, number of seed per pod, seed yield per plant, biomass (gram), 100 seeds weight (gram), harvest index (%), yield per plant. The simple ANOVA for all the characters revealed that difference among genotypes was highly significant. As significant differences were present between the genotypes, it permitted subsequent analysis of the data. The Corresponding author.


Introduction
Lentil (Lens culinaris Medik.) is locally known as Masoor, having ploidy level 2n = 2x = 14 self-pollinated autogamous specie.Owing to small size of floral structure, self fertility is dominated in lentil.It is the 2 nd major Rabi pulse crop both in quality and quantity after chickpea.Lentil is a protein rich (22% -35%), source of crucial amino acids (lysine, tryptophan) soluble and dietary fiber, minerals and vitamins (vitamin A and B).Lentil proteins have significant amount of, limiting amino acid in the cereals [1].In addition to this, medical attributes are associated to it, uses in the remedy of constipation and other intestinal afflictions [2].Being a legumimous crop, it has a unique ability to fix atmospheric nitrogen and more than 85% nitrogen requirements of the crop can be assembled into available from by the bacterial symbiosis [3].In Pakistan, its area of production is decreasing at the alarming rate, considerable reduction in its area from 62,500 hectare to 31,000 hectares and its production from 35,000 tons to 6000 tons [4], putting pressure to fulfill domestic requirements by its heavy import posing huge burden on the rational exchequer from last 10 years, that's why market prices comparatively are eminent which lead to low/capita consumption.Hence its production should be increased by three times at the least to save the money which is now spent on its import and meet the demand of increasing population.
Genetic improvement on the lentil crop has been very melioration and has lacked institutional support.It is imperative to initiate research with the intention of obtaining more productive high-yielding cultivars, particularly through breeding (exploiting local germplasm).Focusing attention on yield and its associated characters, efforts should also be diverted for its different agro-ecological considerations and farming systems.Genetic variance within the populations of crop species is a major concern of plant breeders and geneticists [5].Quantitative attributes render an approximate of genetic diversity; breeders must have potential of selecting the accession that most probably possesses the trait of concern.
The present research work was carried out considering the above facts with focus on objectives: • To access component of variance for type of gene action estimation.
• To access the best combining parents out of four native varieties of Lentil, the estimates would be exploited for further breeding programs.• To produce better lines regarding the high yield for further breeding program of Lentil crop.

Materials and Methods
The study was carried out at the experimental area of Department of Plant Breeding & Genetics, Faculty of Agricultural Science, & Technology, Bahauudin Zakariya University in Multan.Soil status of experimental farm area is given below in Table 1.

Collection of Genotypes
The seeds of four approved genotypes of lentils named as NL-2002, NL-2006, Punjab Masoor-2009 and Markaz-2009 were procured from (NIAB Faisalabad).
All genotypes were sown in the field during spring 2011 and all possible crosses were attempted are listed below in Table 2.

Crossing Procedure
In order to gain the high success rate of artificially induce hybridization only large floral buds were selected.Furthermore, lateral buds were selected instead of terminal ones since the success is generally reported to be high with lateral buds.Selection of such flowers was made which belonged to a particular genotype that had not been self-pollinated and was quite young i.e. the pollens were in the granular form not of powdery form.Subsequently, the sepals were removed followed by removal of anthers containing pollen grains.After emasculation, young flowers of the other genotype containing pollens in powdery form were selected, their sepals and petals were removed around the petioles and finally cutting the end of petioles.Pollen grains were allowed to drain out into the ovary of the tagged flower by pushing its petals aside and the pollinated flower was finally closed.After pollination the solution of Gibberelic acid (10 gm per l liter of H 2 O) is sprayed on crossed plants to recover the injury to plants by crossing equipments.When crop reached on physically maturity, crossed pods were carefully harvested and seeds were threshed manually.At next growing season the F 0 seed along with the parental genotype were sown in Randomized Complete Block Design (RCBD) with three Replications to demonstrate the F 1 generation.Ten to eleven lines, with line to line distance of 1 foot, line length of 10 foot, plant to plant distance of 4 inches and Path length 4 feet.The four genotypes were directly crossed.In each cross, 45 to 90 flowers were pollinated.The 90 flowers of each crosscombination were supposed to be completed in a 10-days time period thus leading to 9 crosses in every 24 hours with 3 crosses from each time interval treatment.

Plant Height
The ANOVA (Analysis of variance) for plant height showed that mean squares for ten genotypes (six crosses and four parents) were highly significant (P ≤ 0.00), which in turn indicated that there were variations in the values for this characters (Table 3).Further analysis of data (as depicted in Table 4) revealed that the values of variance for GCA were rather higher (11.84) than those of SCA (Specific Combining Ability) (5.16).This confirms the role of additive gene effect (V A = 5.16, σ 2 a = 12.9307 ± 1.07) in this character.Similar results were suggested by [6] and [7].
The parents were compared for GCA for plant height character ( For plant height, the potential of parents was analyzed for their specific combinations.The resulting comparisons are shown here in Table 6

Secondary Branches
Significant differences were present (for secondary branches) between the genotypes then it permitted further  analysis of data succeeding combining ability analysis, and the results of analysis are awarded in (Table 10).
The variance resulting from General Combining Ability was higher (24.38) than that because of Specific Combining Ability (−0.98), this shows that variation was pretended by additive gene effects (V A = −0.98,σ 2 a = 33.2715± 1.24).Our findings contradicted the research work of [9] and [2] while the similar results were suggested by [10] and [11].Each of the parents was compared for its general combining ability for the character secondary branches (

Number of Pods per Plant
The variance resulting from General Combining Ability (for number of pods per plant) was higher (1915.38)than that of Specific Combining Ability (327.27),showed that variation was acted by additive gene effects (V A = 327.27,σ 2 a = 3382.091± 1.29 Table 13, Table 14).These results are in line with the findings of [12].18).The potential of parents was analyzed for their specific combinations, and the comparisons are given in (Table 18).The ordering showed that out of six crosses, five combination achieved positive values these are, NL-2002 × NL-2006 (0.17

Biomass
The variance resulting from General Combining Ability was higher (6.59) than that because of Specific Combining   20).Similar results were suggested [13].Each of the parents was compared for its general combining ability for the character Biomass.(Table 21) but further analysis brought out that three parents,

Seed Yield per Plant
Variation was dominated by additive gene effects (V A = 1.44, σ 2 a = 0.050 ± 1.29

100 Seed Weight
General Combining Ability was higher (0.03) than that of Specific Combining Ability (0.00) revealed that variation was pretended by additive gene effects (V A = 0.00, σ 2 a = 0.061 ± 0.19 Table 25, Table 26).The results are in agreement with the findings of [15].Each of the parents was compared for its general combining ability for the character hundred seed weight indicated that NL-2002 (0.25) accomplished positive values and exhibited to

Table 1 .
Soil status of experimental farm area.

Table 3 .
Analysis of variance of plant height in 10 genotypes of Lens culinaris Medik.

Table 4 .
Combining ability analysis of plant height in 10 genotypes of Lens culinaris Medik.

Table 5 .
Means, general and specific combining ability effects of plant height of 4 parents and 6 crosses.
S.E.(gi-gj) = standard error for general combining ability and S.E.(sij-sik) = standard error for specific combining ability, applicable for all subsequent tables.

Table 6 .
Estimate of component of genetic variance in plant height of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 7 .
Combining ability analysis of primary branches in 10 genotypes of Lens culinaris Medik.

Table 8 .
Estimate of component of genetic variance in primary branches of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 9 .
Means, general and specific combining ability effects of primary branches of 4 parents and 6 crosses.

Table 10 .
Combining ability analysis of secondary branches in 4 genotypes of Lens culinaris Medik.

Table 11 .
Means, general and specific combining ability effects of secondary branches of 4 parents and 6 crosses.

Table 12 .
Estimate of component of genetic variance in secondary branches of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 13 .
Combining ability analysis of No. of pods per plant in 10 genotypes of Lens culinaris Medik.

Table 14 .
Estimate of component of genetic variance in No. of pods per plant of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 15 .
Means, general and specific combining ability effects of No. of pods per plant of 4 parents and 6 crosses.

Number of Seed per Pod Non
[12]itivegene effects (V D = −0.19,σ 2 e = 0.042 ± 2.46 Table16, Table17) Similar results were suggested by[12].NL-2002 (0.03) and NL-2006 (0.02), accomplished positive values and demonstrated to be the best general combiner for the character no. of seed per pod and the remaining one parent attained negative value i.e.

Table 16 .
Combining ability analysis of No. of seed per pod in 10 genotypes of Lens culinaris Medik.

Table 17 .
Estimate of component of genetic variance in No. of seed per pod of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 18 .
Means, general and specific combining ability effects of No. of seed per pod of 4 parents and 6 crosses.Ability (2.31), this shows that variation was pretended by additive gene effects (V A = 2.31, σ 2 a = 10.014 ± 0.99

Table 19 .
Combining ability analysis of biomass in 10 genotypes of Lens culinaris Medik.

Table 20 .
Estimate of component of genetic variance in biomass of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 21 .
Means, general and specific combining ability effects of biomass of 4 parents and 6 crosses.Punjab Masoor-2009 (0.39) and Markaz-2009 (1.31) accomplished positive values and exhibited to be the best general combiner for the character seed yield per plant (Table 24).Remaining one parent attained negative value i.e.NL-2002 (−2.30) and clearly showed poor general combining ability for seed yield plant.Ordering showed that out of six crosses, four combination achieved

Table 22 .
Combining ability analysis of seed yield per plant in 10 genotypes of Lens culinaris Medik.

Table 23 .
Estimate of component of genetic variance in Seed yield per plant of Lens culinaris Medik.

Table 24 .
[16]s, general and specific combining ability effects of Seed yield per plant of 4 parents and 6 crosses.thebestgeneralcombinerfor the character hundred seed weight and remaining three parents attained negative value i.e.NL-2006 (−0.21),PunjabMasoor-2009 (−0.02) and Markaz-2009 (−0.03) and clearly showed poor general combining ability for character hundred seed weight.The potential of parents was assessed for their specific combinations, and the comparisons are given (Table27) the ordering showed that out of six crosses, Variation (for harvest index) was pretended by non-additive gene effects (V D = 0.33, σ 2 e = 15.907 ± 2.48 Table28, Table29) owing to lower general Combining Ability was lower (3.75).Similar results were suggested by[16].Comparison of parents for general combining ability for the character harvest index (Table30) indicated that two parents, NL-2002 (0.52) and Markaz-2009 (3.31) accomplished positive values and exhibited to be the be

Table 25 .
Combining ability analysis of 100 seed weight in 10 genotypes of Lens culinaris Medik.

Table 26 .
Estimate of component of genetic variance of 100 seed weight of Lens culinaris Medik.
σ 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect.

Table 27 .
Means, general and specific combining ability effects of 100 seed weight of 4 parents and 6 crosses.

Table 28 .
Combining ability analysis of harvest index in 10 genotypes of Lens culinaris Medik.

Table 29 .
Estimate of component of genetic variance in harvest index of Lens culinaris Medik. 2 a = variance due to additive gene effect; σ 2 e = variance due to environmental effect. σ