Prof. Jean Sévigny
Department of Microbiology-Infectiology and Immunology
Université Laval, Canada
Email:Jean.Sevigny@crchul.ulaval.ca
Qualifications
2010 Professor, Dept. of Microbiology-Infectiology and Immunology, Laval University
2009 Associate Professor, Dept. of Anatomy & Physiology, Laval University
2006 Assistant Professor, Dept. of Anatomy & Physiology, Laval University
1997 Ph.D., Biology, Sherbrooke University
1992 M.Sc., Microbiology, Sherbrooke University
1988 B.Sc., Biology (Biotechnology), Sherbrooke University
Publications (Selected)
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Enjyoji, K., J. Sévigny, Y. Lin, P.S. Frenette, P. D.
Christie, J. Schulte am Esch II, M. Imai, J.M. Edelberg, H. Rayburn, M. Lech,
D.L. Beeler, E. Csizmadia, D.D. Wagner, S.C. Robson & R.D. Rosenberg. (1999) Targeted disruption of cd39/ATP diphosphohydrolase results in
disordered hemostasis and thromboregulation. Nat. Med.5(9):
1010-1017.
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Sévigny, J., S.C. Robson, E. Waelkens,
E. Csizmadia, R.N. Smith & R. Lemmens. (2000) Identification and characterization of a novel
hepatic canalicular ATP diphosphohydrolase. J. Biol. Chem.275(8): 5640-5647.
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Sévigny,
J., C. Sundberg, N. Braun, O. Guckelberger, E.
Csizmadia, I. Qawi, M. Imai, H. Zimmermann & S.C. Robson. (April 152002)
Differential catalytic properties and vascular topography of murine nucleoside
triphosphate diphosphohydrolase 1 (NTPDase1) and NTPDase2 have implications for
thromboregulation.Blood99(8) :
2801-2809.
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Dranoff, J. A., E. A. Kruglov, S.C. Robson, N.
Braun, H. Zimmermann &J. Sévigny.(2002) Theecto-nucleoside
triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel
functional compartment within the liver.HepatologyNovember36(5): 1135-1144.
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Lavoie,
E. G., F.
Kukulski, S. A. Lévesque, J. Lecka&J. Sévigny.(2004) Cloning
and characterization of mouse nucleoside triphosphate diphosphohydrolase-3.Biochem.
Pharmacol.67(10): 1917-1926.
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Bigonnesse, F., S. A. Lévesque, F. Kukulski, J. Lecka, S.C.
Robson, M.J.G. Fernandes&J. Sévigny.(2004) Cloning and characterization of mouse
nucleoside triphosphate diphosphohydrolase-8. Biochemistry43(18): 5511-5519.
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Kittel, A., J.
Pelletier, F. Bigonnesse, O. Guckelberger, K. Kordás, N. Braun, S.C.
Robson&J. Sévigny.(2004)Localization
of nucleoside triphosphate diphosphohydrolase-1 (NTPDase1) and NTPDase2 in
pancreas and salivary gland.J. Histochem. Cytochem.52(7): 861-871.
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Dranoff,
J. A., E.A. Kruglov, J. Toure, N. Braun, H. Zimmermann, D. Jain, A.F. Knowles
&J. Sévigny.(2004) Ectonucleotidase NTPDase2 is selectively
down-regulated in biliary cirrhosis.Journal of Investigative Medicine52(7): 475-482.
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Kukulski, F., S. A. Lévesque, E.G. Lavoie, J. Lecka, F. Bigonnesse,
A. F. Knowles, S.C. Robson, T. L. Kirley&J. Sévigny.(2005) Comparative hydrolysis of P2
receptor agonists by NTPDases 1, 2, 3 and 8.Purinergic Signalling1(2): 193-204.
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Bartel,
D. L., S.L. Sullivan, E. G. Lavoie, J.
Sévigny& T.E. Finger.(2006) Nucleoside triphosphate
diphosphohydrolase-2 (NTPDase2) is the ecto-ATPase of type I cells in taste
buds.J. Comp. Neurol.497(1): 1-12.
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Fausther,
M., J. Lecka, F. Kukulski, S. A. Lévesque, J. Pelletier, H. Zimmermann,
J. A. Dranoff&J. Sévigny.(2007) Cloning, purification, and identification of the liver
canalicularecto-ATPase as NTPDase8. Am.
J. Physiol.Gastrointest.
Liver Physiol.292(3): G785-95.
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Kukulski, F., F. Ben Yebdri, J. Lefebvre, M. Warny, P. A. Tessier &J. Sévigny.(2007) Extracellular nucleotides mediate LPS-induced
neutrophil migration in vitro and in vivo. J. Leukoc. Biol.81(5): 1269-75.
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Lévesque,
S.A., E. G.
Lavoie, J. Lecka, F. Bigonnesse&J. Sévigny.(2007) Specificity of the ecto-ATPase inhibitor ARL 67156 on major
ecto-nucleotidases. Br. J. Pharmacol.152(1): 141-50.
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Munkonda,
M. N., G.
Kauffenstein, F. Kukulski, S. A. Lévesque, C. Legendre, J. Pelletier, E.G. Lavoie, J. Lecka&J. Sévigny.(2007) Inhibition of human and
mouse plasma membrane bound NTPDases by P2 receptor antagonists. Biochem. Pharmacol.74: 1524-34.
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Munkonda, M.N., J. Pelletier, V.V. Ivanenkov, M. Fausther, A. Tremblay, B.
Künzli, T.L. Kirley&J. Sévigny.(2009)Characterization of a
monoclonal antibody as the first specific inhibitor of human NTP
diphosphohydrolase-3 - Partial characterization of the inhibitory epitope and
potential applications.FEBS J.276:479-96.
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Martín-Satué,
M., É.G. Lavoie, J. Pelletier, M. Fausther, E. Csizmadia, O. Guckelberger, S.C. Robson&J. Sévigny.(2009; epub 6 jn)Localization of plasma membrane bound NTPDases in the murine
reproductive tract. Histochem. Cell. Biol.131(5):615-28.
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Kukulski, F., F. Ben Yebdri, J. Lecka, G. Kauffenstein,S.A.
Lévesque, M. Martín-Satué&J. Sévigny.(2009) Extracellular ATP and P2 receptors are required for IL-8 to
induce neutrophil migration. Cytokine46(2): 166-70.
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Ben Yebdri, F., F. Kukulski, A. Tremblay&J. Sévigny.(Oct2009)Concomitant activation of P2Y2and P2Y6receptors onmonocytes is required for TLR1/2-induced neutrophilmigration by
regulating IL-8 secretion.Eur. J. Immunol.39(10):2885-94.
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Friedman,
D.J., B.M. Künzli, Y.I. A-Rahim, J. Sévigny,
P.O. Berberat, K.Enjyoji, E. Csizmadia, H.Friess& S.C. Robson.(2009)CD39 deletion exacerbates experimental murine
colitis and human polymorphisms increase susceptibility to inflammatory bowel
disease.PNAS106(39): 16788-93.
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Kukulski,
F., F. Ben
Yebdri, F. Bahrami, S. A. Lévesque, M. Martín-Satué&J. Sévigny.(2010)TheP2 receptor antagonist PPADS abrogates LPS-induced neutrophil
migration in the murine air pouch via inhibition of MIP-2 and KC production. Mol. Immunol.47(4): 833-9.
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Kauffenstein,
G., A. Drouin, N.
Thorin-Trescases, H. Bachelard, B. Robaye, P. D’Orléans-Juste, F. Marceau, É.
Thorin&J. Sévigny.(2010) NTPDase1 (CD39) controls
nucleotide-dependent vasoconstriction in mouse. Cardiovasc. Res.85(1): 204-13.
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Kukulski,
F., F. Ben
Yebdri, F. Bahrami, M. Fausther, A. Tremblay&J. Sévigny.(2010) Endothelial P2Y2receptor
regulates LPS-induced neutrophil transendothelial migrationin vitro. Mol. Immunol.47(5): 991-9.
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Kauffenstein,
G.,C. R. Fürstenau,
P. D’Orléans-Juste&J. Sévigny.(2010). The ecto-nucleotidase NTPDase1 differentially regulates P2Y1and P2Y2receptor-dependent vasorelaxation. Br. J. Pharmacol.159(3): 576-85.
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Eliahu, S., A. Martín-Gil, M.J. Perez de
Lara, J. Pintor, J. Camden, G.A. Weisman, J. Lecka,J. Sévigny&
B. Fischer. (2010) 2-MeS-β, γ-CCl2-ATP
is a potent agent for reducing intraocular pressure.J. Med.Chem.53(8): 3305-19.
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Lévesque,
S. A., F.
Kukulski, K. Enjyoji, S.C. Robson&J. Sévigny.(2010) NTPDase1 governs P2X7-dependent
functions in murine macrophages. Eur. J. Immunol.40(5):
1473-85.
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Martín-Satué,
M., E.G. Lavoie, M. Fausther, J. Lecka, E. Aliagas, F. Kukulski&J. Sévigny.(2010) High expression and activity of
ecto-5’-nucleotidase/CD73 in the male murine reproductive tract. Histochem. Cell. Biol.133(6): 659-68.
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Fausther,
M., J. Pelletier,
C. M. Ribeiro, J. Sévigny& M.
Picher.(2010)Cystic fibrosis remodels the regulation of
purinergic signaling by NTPDase1 and NTPDase3.Am. J. Physiology - Lung Cell. Mol. Physiol.298(6):L804-18.
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Lavoie, E. G., M.
Fausther,G. Kauffenstein,F. Kukulski, B.M. Künzli, H.
Friess&J. Sévigny.(2010) Identification of the ectonucleotidases
expressed in mouse, rat and human Langerhans islets: Potential role of NTPDase3
ininsulin secretion. Am. J. Physiol. Endocrinol. Metab.299(4): E647-56.
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Lecka, J., M.anjitS.inghRana &J. Sévigny.(Nov2010; epub) Inhibition of vascular
ectonucleotidase activities by the pro-drugs ticlopidine and clopidogrelfavours
platelet aggregation. Br.J. Pharmacol.161(5): 1150-60.
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Eliahu, S., J. Lecka, G. Reiser, M.
Haas,F. Bigonnesse, S. A. Lévesque, J. Pelletier&[Co senior authors J. Sévigny, B. Fischer]. (2010)Diadenosine-5',5''-(boranated)polyphosphonate
analogues as selective Nucleotide
Pyrophosphatase/Phosphodiesterase(NPP)inhibitors.J. Med. Chem.53(24): 8485-97.
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Lavoie, E.G., B.D. Gulbransen, M.
Martín-Satué, E. Aliagas, K. A. Sharkey &J. Sévigny.(2011)Ectonucleotidases in the digestive system:focus
on NTPDase3 localization.Am. J. Physiol. Gastrointest. Liver
Physiol.300(4): G608-20.
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Kukulski, F., F. Bahrami, F.
Ben Yebdri, J. Lecka, M. Martín-Satué, S. A. Lévesque&J. Sévigny.(2011)NTPDase1 controls IL-8 production by human
neutrophils. J.
Immunol.187(2): 644-53.
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Fausther, M., J. Lecka, E. Soliman, G.Kauffenstein, J. Pelletier, N. Sheung, J.A.
Dranoff&J. Sévigny.(2012)
Co-expression of ecto-5'-nucleotidase/CD73 with specific NTPDases
differentially regulates adenosine formation in the rat liver.Am. J. Physiol. Gastrointest. Liver Physiol.302: G447-59.
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Braganhol, E., R.F.
Zanin, A. Bernardi, L.S. Bergamin, A.R. Cappellari, L.F. Campesato, F.B.
Morrone, M.M. Campos, J.B. Calixto, M.I.A. Edelweiss, M.R. Wink,J. Sévigny, S.C. Robson & A.M.O.
Battastini. (2012)Overexpression of
CD39L1/NTPDase2 in gliomas promotes systemic inflammation and pulmonary injury.Purinergic
Signalling8(2): 235-43.
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Lecka, J., I. Gillerman, M. Fausther, M. Salem, M.N. Munkonda, J.-P. Brosseau, C. Cadot, M. Martín-Satué, P.
d'Orléans-Juste, É. Rousseau, D. Poirier, B. Künzli, B. Fischer&J.
Sévigny.(2013) 8-BuS-ATP
derivatives as specific NTPDase1 inhibitors.Br.J. Pharmacol.169(1): 179-96.
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Jenabian, M.-A., N, Seddiki, A, Yatim, M,
Carriere, A, Hulin, M, Younas, E, Ghadimi, A, Kok, J.-P. Routy, A. Tremblay, J. Sévigny, J.-D. Lelievre&Y.
Levy. (2013) Regulatory T
cells negatively affect IL-2 production of effector T cells through
CD39/Adenosine pathway in HIV infection.PLoS
Pathogens9(4): e1003319.
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Lecka,
J., G. Ben-David,
S. Eliahu, J. Pelletier, J. Oscar Jr., P. Luyindula, B.
Fischer, H. Senderowitz &J. Sévigny.(2013) Non-hydrolysable ATP
analogs as selective inhibitors of human NPP1: A combined computational/experimental study.J.
Med. Chem.56(21): 8308-20.
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Lecka, J., M. Fausther, M.
Martín-Satué&J. Sévigny.(2014)
CD39 may impact clopidrogrel pharmacodynamics. Austin J. Clin. Immunol. 1(1): 1-2.
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Nadel, Y., J. Lecka, Y.
Gilad, G. Ben-David, D. Förster, G. Reiser, S. Kenigsberg, J. Camden, G.A.
Weisman,[Co senior authorsH. Senderowitz, J. Sévigny, B. Fischer]. (2014) Highly potent and selective ectonucleotide pyrophosphatase/phosphodiesterase
I inhibitors based on an adenosine5’-(α or γ)-thio-(α,β or β,γ)-methylenetriphosphate
scaffold.J. Med. Chem. 57(11): 4677-91. doi: 10.1021/jm500196c.
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Bahrami, F., F.
Kukulski, J. Lecka, A. Tremblay, J.
Pelletier, L. Rockenbach&J.
Sévigny.(2014)
Purine metabolizing ectoenzymes
control IL-8 production in human colon HT-29 cells. Mediators of
Inflammation.vol.
2014, Article ID879895,10 pages. doi:10.1155/2014/879895.
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Rockenbach,
L., E. Braganhol,
F. Dietrich, F. Figueiró, M. Pugliese, M. I. A. Edelweiss, F. B. Morrone, J. Sévigny&A.M.O.
Battastini. (2014) NTPDase3 and
ecto-5’-nucleotidase/CD73 are differentially expressed during mouse bladder
cancer progression. Purinergic Signalling.10(3): 421-30.
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Gillerman, I., J. Lecka, L. Simhaev, M.N.
Munkonda, M. Fausther, M. Martín-Satué&[Co senior authors H. Senderowitz, J.
Sévigny, B. Fischer].
(2014) 2-Hexylthio-β, γ-CH2-ATP
is an effective and selective NTPDase2 inhibitor. J. Med. Chem.57(14): 5919-34.
doi: 10.1021/jm401933c.
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Lecka, J., M.
Fausther, Beat Künzli&J.
Sévigny.(2014) Ticlopidine
in its prodrug form is a selective inhibitor of human NTPDase1. Mediators of Inflammation. vol. 2014, Article ID547480, 8 pages. doi:10.1155/2014/547480.
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Kauffenstein,
G., J. Pelletier, E. G. Lavoie, F. Kukulski, M. Martín-Satué, S. S.
Dufresne, J. Frenette, C. Ribas Fürstenau, M.J. Sereda, B. Toutain, D. Henrion, R. Sullivan, C. Vial &J. Sévigny.(2014) Nucleoside triphosphate diphosphohydrolase-1
ectonucleotidase is required for normal vas deferens contraction and male
fertility through maintaining P2X1 receptor function.J. Biol. Chem. 289(41):
28629-39.
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Braganhol, E., F. Kukulski, S. A. Lévesque, M.
Fausther, E. G. Lavoie, A. Zanotto-Filho, L. S. Bergamin, J.
Pelletier, F. Bahrami, F. Ben Yebdri, J.C. Moreira, A.M.O.
Battastini&J. Sévigny.(2015) Nucleotide
receptors control IL-8/CXCL8 and MCP-1/CCL2 secretions as well as proliferation
in human glioma cells. Biochim. Biophys.
Acta-Molecular Basis of Disease. 1852(1): 120-30. doi:
10.1016/j.bbadis.2014.10.014.
Profile Details
Last Updated: July 7, 20115.
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