Biography

Dr. Norman H. L. Chiu

The University of North Carolina at Greensboro, USA

Associate Professor


Email: prof.chiu@gmail.com


Qualifications

2001 Postdoc., Boston University & Sequenom Inc., Biological Mass Spectrometry

1998 Ph.D., University of Windsor, Biochemistry

1992 M.Sc., University of Bristol, Analytical Chemistry

1990 B.Sc., University of Liverpool, Chemistry


Publications (Selected)

  1. Christopoulos TK, Chiu NH. Expression Immunoassay. Antigen quantitation using antibodies labeled with enzyme-coding DNA fragment. Anal. Chem., 67; 4290 (1995).
  2. Chiu NH, Christopoulos TK. Hybridization assays using as a label an expressible DNA fragment encoding firefly luciferase. Anal. Chem., 68; 2304 (1996).
  3. Chiu NH, Christopoulos TK, Peltier J. Sandwich-type DNA hybridization assays based on enzyme amplified time-resolved fluorometry. Analyst, 123; 1315 (1998).
  4. White SR, Chiu NH, Christopoulos TK. Expression immunoassay based on antibodies labeled with a DNA fragment encoding the alpha-peptide of beta-galactosidase. Analyst, 123; 1309 (1998).
  5. Chiu NH*, Cantor CR. Mass spectrometry of nucleic acids. Clin. Chem., 45; 1578 (1999).
  6. Chiu NH, Christopoulos TK. Two-site expression immunoassay using firefly luciferase-coding DNA label. Clin. Chem., 45; 1954 (1999).
  7. Chiu NH*, Tang K, Yip P, Braun A, Koster H, Cantor CR. Mass spectrometry of single-stranded restriction fragments captured by an undigested complementary sequence. Nucleic Acids Res., 28; e31 (2000).
  8. White SR, Chiu NH, Christopoulos TK. Expression immunoassay. Methods, 22; 24 (2000).
  9. Leushner J, Chiu NH. Automated mass spectrometry: a revolutionary technology for clinical diagnostics [review]. Mol. Diagn. 5; 341(2000)
  10. Shahgholi M, Garcia BA, Chiu NH, Heaney PJ, Tang K. Sugar additives for MALDI matrices improve signal allowing the smallest nucleotide change (A:T) in a DNA sequence to be resolved. Nucleic Acids Res., 29; e91 (2001).
  11. Tannous B, Chiu NH, Christopoulos TK. Heterobifunctional linker between antibodies and reporter genes for immunoassay development. Analytica Chimica Acta, 459, 169 (2002).
  12. Wintzingerode FV, Bocker S, Schlotelburg C, Chiu NH, Storm N, Jurinke C, Van den Boom D. Base-specific fragmentation of amplified 16S rRNA genes and mass spectrometry analysis: A novel tool for rapid bacterial identification. Proc. Nat. Acad. Sci ., 99, 7039 (2002).
  13. S. Schiavo, A. El-Shafey, N. Jordan, C. Orazine, Y. Wang, X. Zhou, N.H. Chiu, and I.S. Krull. Immuno-PCR: an overview of the analytical technique currently pushing the limits of detection [review]. PharmaGenomics. January, 36-45 (2004).
  14. Li G, Zhou X, Wang Y, El-Shafey A, Chiu NH, and Krull IS. Capillary isoelectric focusing and affinity capillary electrophoresis approaches for the determination of binding constants for antibodies to the prion protein. J. Chromatogr A, 1053(1/2), 253-262 (2004).
  15. Schiavo S, Yang W, Chiu NH and Krull IS. Comparison of fluorometric detection methods for quantitative PCR. J Immunoassay Immunochem, 26(1), 1-12 (2005).
  16. Littman M, Chiu NH and Wang J. Specific recognition of non-denatured nitrite-oxidizing system of Nitrospira moscoviensis by monoclonal antibody Hyb 153-3. J Chem Technol Biotechnol 81 (3), 318-21 (2006).
  17. Qing L, Chiu NH and Vouros P. Investigation of benzonase/alkaline phosphatase digestion of DNA and DNA-adducts using LC-ESI MS. Anal. Chem., 79(5), 1907-17 (2007).
  18. Barnes CA, and Chiu NH. Accurate characterization of carcinogenic DNA adducts using MALDI tandem time-of-flight mass spectrometry. Int. J. Mass Spectrom., 279, 170-175 (2009)
  19. Yang W, Chiu NH. Correlation between UV spectrophotometry and quantitative MALDI-TOF MS. Spectroscopy Letters, 43(7), 602-608, (2010).
  20. Xiaojiao Zheng, Guoxiang Xie, Aihua Zhao, Chun Yao, Weiping Jia, Norman H. L. Chiu, Zhanxiang Zhou, Wei Jia. The footprints of gut microbial - mammalian co-metabolism. Journal of Proteome Research 10(12), 5512-5522 (2011).
  21. Jack G. Walleshauser III, Trace Kessler, Bakhos A. Tannous, Norman H. L. Chiu. A Simple Approach for Evaluating Total MicroRNA Extraction from Mouse Brain Tissues. Journal of Analytical Sciences, Methods and Instrumentation (2012) Accepted.
  22. N.H Chiu, T. K. Christopoulos. “Advances in Immunoassay Technology”, InTech, (In Press).
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