Prof. Roberto Fernández Lafuente
Consejo Superior de Investigaciones
Científicas, Spain
Email: rfl@icp.csic.es
Qualifications
Ph.D. in Sciences
by UAM
Master's Degree in Biology
by UCM
Publications (selected)
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Rosell, C.M., Fernández Lafuente, R. & Guisán,
J.M. 1995. "Modification of enzyme properties by the use of inhibitors
during their stabilization by multipoint covalent attachment".
Biocatalysis & Biotransformations. 12, 67-76.
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Wood, A..N.P., Fernández-Lafuente, R.,
Cowan. D.A. 1995. "Purification and partial characterisation of a novel
thermophilic esterase with high mesophilic specific activity" Enzyme
Microb. Technol. 17, 816-825.
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Fernández-Lafuente, R., Wood, A.N.P. & Cowan,
D.A. 1995. "Reducing enzyme conformational flexibility by multipoint
covalent immobilisation" Biotechnol. Techniques. 9, 1-6.
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Wood, A.N.P., Fernández-Lafuente, R. & Cowan,
D.A. 1995. "Characterisation of a novel thermophilic esterase as an
industrial catalyst" Biotechnol. Appl. Biochem. 2,: 313-322.
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Bes, T., Gómez-Moreno, Guisán, J.M. &
Fernández-Lafuente, R*. 1995. "Selective enzymic oxidations: Stabilisation
by multipoint covalent attachment of ferredoxin NADP-reductase, an interesting
cofactor recycling enzyme". J. Mol. Cat. 98, 161-169.
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Fernández-Lafuente, R., Rosell, C.M. & Guisán,
J.M. 1995."The use of stabilised penicillin G acylase derivatives improves
the design of kinetically controlled synthesis". J. Mol. Cat,. 101,
91-97.
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Fernández-Lafuente, R., Rosell, C.M.,
Piatkowska, B. & Guisán,J.M. 1996. "Synthesis of antibiotics
(CEPHALOGLYCIN) catalysed by penicillin G acylase: evaluation and optimization
of different synthetic approaches". Enzyme Microb. Technol. 19, 9-14.
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Fernández-Lafuente, R., Rosell, C.M. & Guisán,
J.M. 1996. "Dinamic reaction design of enzymic biotransformations in
organic media: equilibrium controlled synthesis of antibiotics by penicillin G
acylase". Biotechnol. Appl Biochem. 24, 139-143.
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Soler, G., Bastida, A., Blanco, R.M.,
Fernández-Lafuente, R & Guisán, J.M. 1997. "Reactivation
strategies by unfolding/refolding of chymotrypsin derivatives after
inactivation by organic solvents" Biochim. Biophys. Acta. 1339, 167-175
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Fernández-Lafuente, R., Guisán, J.M., Pregnolato,
M. & Terreni, M. 1997. “Chemoenzymatic one pot synthesis of cefazolin from
cephalosporin C in fully aqueous medium involving three consecutive
biotransformations catalyzed by D-aminoacid oxidase, glutaryl acylase and
Penicillin G acylase” Tetrahedrum Lett. 38: 4693-4696.
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Hernández-Justiz, O., Fenández-Lafuente, R.,
Guisán, J.M.; Negri, P., Pagani, G., Pregnolato. M. & Terreni, M.
1997. “One pot chemoenzymatic synthesis of 3´functionalized cephalosporins
(cephazolin) by three consecutive biotransformations in fully aqueous medium”
J. Org, Chem. 62, 9099-9106.
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Bastida, A., Sabuquillo, P., Armisen, P.,
Fernández-Lafuente, R., Huguet, J. & Guisán. J.M. 1998. “A single step
purification, immobilization and hyperactivation of lipases via interfacial
adsorption on strongly hydrophobic supports” Biotechnol. Bioeng. 58, 486-493.
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Hernández, O, Fernández-Lafuente, R.,
Terreni, M. & Guisán, J.M. 1998. “Immobilized enzymes and aqueous two-phase
systems: Continuos extraction of water soluble products (cephalexin) during antibiotic
synthesis by penicillin acylase”. Biotechnol. Bioeng. 59, 73-79
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Fernández-Lafuente, R., Rosell, C.M. & Guisán,
J.M. 1998. “Modulation of the properties of penicillin G acylase by acyl donor
substrates during N-protection of amino compounds” Enzyme Microb. Technol. 22,
583-587.
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Fernández-Lafuente, R., Rodríguez, V. &
Guisán, J.M. 1998. “The coimmobilization of D-aminoacid oxidase and catalase
enables the quantitative transformation of D-amino acids (phenylalanine) into
alpha-ceto acids (phenylpyruvic acid). Enzyme Microb. Technol. 23, 28-33.
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Rosell, C.M., Terreni, M., Fernández-Lafuente, R*.
& Guisán, J.M* 1998. “A criterium for the selection of monophasic solvents
for enzymatic synthesis”. Enzyme Microb. Technol. 23, 64-69
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Fernández-Lafuente, R., Armisen, P., Sabuquillo,
P., Fernández-Lorente, G. & Guisán, J.M. 1998. “Immobilization of lipases
by selective adsorption on hydrophobic supports” Chem. Phys. Lipids. 93,
185-197.
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Fernández-Lafuente, R., Rosell, C.M. & Guisán,
J.M. 1998. “The presence of methanol exerts a strong and complex modulation of
the synthesis of different antibiotics by immobilized penicillin G acylase”
Enzyme Microb. Technol. .23, 305-310.
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Penzol, G., Armisen, P., Fernández-Lafuente, R.,
Rodes, L. & Guisán, J.M. 1998. “Use of dextrans as long, inert and
hydrophilic spacer arms to improve the performance of immobilized proteins
acting on macromolecules” Biotechnol. Bioeng. 60, 518-523..
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Ali, S., Fernández-Lafuente, R. & Cowan,
D.A 1998. “Metha-pathway of phenol by thermophilic Bacilli”. Enzyme Microb.
Technol. 23, 462-468.
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