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  • 348pp. Published September 2018
  • Scientific Research Publishing,Inc.,USA
  • Category: Biomedical & Life Sciences
  • ISBN: 978-1-61896-606-3
  • (Hardcover) USD 109.00
  • ISBN: 978-1-61896-605-6
  • (Paperback) USD 89.00
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Home > Books > Advances in Super-resolution Microscopy
Advances in Super-resolution Microscopy
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Super-resolution microscopy, in light microscopy, is a term that gathers several techniques, which allow images to be taken with a higher resolution than the one imposed by the diffraction limit. Due to the diffraction of light, the resolution in conventional light microscopy is limited, as stated (for the special case of widefield illumination) by Ernst Abbe in 1873. In this context, a diffraction-limited microscope with numerical aperture N.A. and light with wavelength λ reaches a lateral resolution of d = λ/(2 N.A.) - a similar formalism can be followed for the axial resolution (along the optical axis, z-resolution, depth resolution). The resolution for a standard optical microscope in the visible light spectrum is about 200 nm laterally and 600 nm axially. Experimentally, the attained resolution can be measured from the full width at half maximum (FWHM) of the point spread function (PSF) using images of point-like objects. Although the resolving power of a microscope is not well defined, it is generally considered that a super-resolution microscopy technique offers a resolution better than the one stipulated by Abbe.

In the present book, twelve typical literatures about super-resolution microscopy published on international authoritative journals were selected to introduce the worldwide newest progress, which contains reviews or original researches on Face Hallucination Super Resolution, Sparse-coding Super-Resolution, Structured illumination microscopy, Stimulated emission depletion, Localization microscopy and Omnipresent Localization Microscopy, etc. We hope this book can demonstrate advances in Super-resolution microscopy as well as give references to the researchers, students and other related people.

Components of the Book:
  • Chapter 1
    Super-resolution fluorescence microscopy studies of human immunodeficiency virus
  • Chapter 2
    Super-resolution microscopy reveals γ-secretase at both sides of the neuronal synapse
  • Chapter 3
    From single molecules to life: microscopy at the nanoscale
  • Chapter 4
    3D structured illumination microscopy of mammalian embryos and spermatozoa
  • Chapter 5
    Performance of four HRP-2/pLDH combination rapid diagnostic tests and field microscopy as screening tests for malaria in pregnancy in Indonesia: a cross-sectional study
  • Chapter 6
    Correlative Microscopy—Novel Methods and Their Applications to Explore 3D Chemistry and Structure of Nanoscale Lattice Defects: A Case Study in Superalloys
  • Chapter 7
    Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study
  • Chapter 8
    Platelet hyperactivity and fibrin clot structure in transient ischemic attack individuals in the presence of metabolic syndrome: a microscopy and thromboelastography study
  • Chapter 9
    Anti-inflammatory cellular targets on neutrophils elucidated using a novel cell migration model and confocal microscopy: a clinical supplementation study
  • Chapter 10
    Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development
  • Chapter 11
    Malaria over-diagnosis in Cameroon: diagnostic accuracy of Fluorescence and Staining Technologies (FAST) Malaria Stain and LED microscopy versus Giemsa and bright field microscopy validated by polymerase chain reaction
  • Chapter 12
    Costs and cost-effectiveness of malaria reactive case detection using loop-mediated isothermal amplification compared to microscopy in the low transmission setting of Aceh Province, Indonesia
Readership: Students, academics, teachers and other people attending or interested in super-resolution microscopy.
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