Determination of Ursolic Acid a Biomarker in Different Swertia Species through High Performance Thin Layer Chromatography

Mansi Gupta, Deepa Bisht, Sayyada Khatoon, Sharad Srivastava, Ajay Kumar Singh Rawat

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DOI: 10.4236/cm.2011.24020                

Abstract

A simple highly precise method has been established for the determination of ursolic acid in three species of Swertia viz. S. angustifolia, S. paniculata, and S. alata. The assay combines identification and quantification of the analyte on silica gel 60GF₂₅₄ HPTLC plates with visualization under UV light and scanning at 510 nm.

Keywords

Ursolic Acid, Swertia Species, HPTLC

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	S. Garg, “International Bioscience Monograph,” Today & Tomorrow’s Printers & Publishers, India, 1987.
[2]	Anonymous, “The Wealth of India, a Dictionary of Indian Raw Materials and Industrial Products,” Council of Scientific and Industrial Research, New Delhi, 1976, pp. 77-81.
[3]	R. N. Chopra, S. L. Nayar and I. C. Chopra, “Glossary of Indian Medicinal Plants,” CSIR, New Delhi, 1956, p. 237.
[4]	B. D. Naithani, “Flora of Chamoli, Botanical Survey of India,” Botanical survey of India, Calcutta, 1985, p. 433.
[5]	U. Dhar and P. Kachroo, “Alpine Flora of Kashmir Himalaya,” Scientific Publishers, Jodhpur, 1983, p. 132.
[6]	S. P. Ambasta, “The Useful Plants of India,” Publication & Information Directorate, CSIR, New Delhi, 1986, pp. 608-609.
[7]	K. S. Khetwal and R. S. Bisht, “A Xanthone Glycoside from Sweria speciosa,” Phytochemistry, Vol. 27, No. 6, 1988, pp. 1910-1911. doi:10.1016/0031-9422(88)80481-3
[8]	A. K. Chakravarty, S. Mukhophadhyay, K. Masuda and H. Ageta, “More of Swertane Triterpenoids from S. chirayita Buch-Ham,” Indian Journal of Chemistry B, Vol. 31, 1992, pp. 70-71.
[9]	S. Ghosal, P. V. Sharma, R. K. Chaudhuri and S. K. Bhodtacharya, “Chemical Constituents of the Gentianaceae-V: Tetraoxygenated Xanthones of Sweria chirayita Buch-Ham,” Journal of Pharmaceutical Sciences, Vol. 62, No. 6, 1973, pp. 926-930. doi:10.1002/jps.2600620614
[10]	A. K. Chakravarty, S. Mukhopadhyay, S. K. Moitra and B. Das, “Syrungareinol, a Hepatoprotective Agent and Other Constituents from Sweria chirayita,” Indian Journal of Chemistry B, Vol. 33, 1994, pp. 405-408.
[11]	P. K. Chaudhuri and W. M. Daniewshi, “Unambigous Assignments of the 1H and 13C Chemical Shifts of the Major Bitter Principles of Sweria chiraita by 2D-NMR Study and Characterization of Other Constituents,” Polish Journal of Chemistry, Vol. 69, 1996, pp. 1514-1519.
[12]	A. K. Chakravarty, S. Mkhopadhyay and B. Das, “Swertane Terpenoids from Swertia chirayita,” Phytochemistry, Vol. 30, 1991, pp. 4092-4087.
[13]	V. S. Rana and M. S. M. Rawat, “A New Xantone Glycoside and Antioxidant Constituents from the Rhizomes of Sweria speciosa,” Chemistry and Biodiversity, Vol. 2, No. 10, 2005, pp. 1310-1315. doi:10.1002/cbdv.200590102