Nucleotide Sequence Variations in a Medicinal Relative of Asparagus, Asparagus cochinchinensis (Lour.) Merrill (Asparagaceae)

Tatsuya Fukuda, In-Ja Song, Takuro Ito, Hiroshi Hayakawa, Yukio Minamiya, Akira Kanno, Hokuto Nakayama, Jun Yokoyama

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DOI: 10.4236/ajps.2011.26091                

Abstract

To determine the eVolutionary history of Asparagus cochinchinensis (Asparagaceae), we investigated the geographic pattern of its nucleotide sequence variations in Japan, Taiwan, South Korea and China. We found 21 polymorphic nucleotide sites by sequencing the internal transcribed spacer (ITS) region, which gave rise to a total of 15 haplotypes, labeled A to O. The A-type was found only in inland China (Guizhou and Sichuan), and the other haplotypes in China extended to several lineages; therefore, A. cochinchinensis may have its origin in the interior of China. The I-type has large distribution area and it also experienced a quick expansion in relative recent years. Haplotype differentiation was observed between the eastern and western side of the Central Mountain Ridge in Taiwan. Two lineages were found in Japan, one in the Yaeyama Islands and the other in remaining areas of Japan, implying that A. cochinchinensis independently colonized in Japan at least twice.

Keywords

Asparagus cochinchinensis, Internal Transcribed Spacer (ITS), Phylogeny, Phylogeography

Conflicts of Interest

The authors declare no conflicts of interest.

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