Producing single microbubbles with controlled size using microfiber

Shinji Deguchi, Satoshi Takahashi, Syuhei Tanimura, Hidenori Hiraki

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DOI: 10.4236/abb.2011.25056                

Abstract

Microbubble-mediated pore formation in sonoporation includes a combination of complex processes such as collision or coalescence of translating or collapsing microbubbles with vascular cells. Although understanding the pore formation mechanisms may improve drug delivery efficiency, their details are still poorly understood. In the present study, we describe an experimental model that produces single air bubbles with controllable size. A carbon microfiber in liquids is illuminated by an infrared laser to produce individual bubbles having size comparable to that of the microfiber. The microbubbles can be physically isolated from the fiber for placing at arbitrary positions in the liquids. The lifetime of the bubbles is several tens of minutes depending on the intensity of the laser used. The preparation of the controllable air bubbles may be useful in future investigations of ultrasound-mediated microbubble–cell interactions.

Keywords

Microbubble; Nanobubble; Carbon Fiber; Glass Fiber; Infrared Laser; Drug Delivery System

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	[1] Yamano, I., Tamagawa, M. (2006) Deformation analysis of bubble near curved elastic wall for developing shock wave DDS. JSME Int. J. Ser. B 49, 755-760.
[2]	Shirota, T., Sanada, T., Arata, Y., Watanabe, M., Kameda, M. (2007) Formation of single bubble of sub-millimeter size using pulsed pressure fluctuation of gas. J. Jpn. Soc. Mech. Eng. B 73, 467-473.
[3]	Shintaku, H., Imamura, S., Kawano, S. (2008) Microbubble formations in MEMS-fabricated rectangular channels: A high-speed observation. Exp. Therm. Fluid Sci. 32, 1132-1140.
[4]	Shirota, M., Imamura, T., Kameda, M. (2008) Formation of single bubbles from a submerged orifice using pulsed ultrasound waves. J. Fluid Sci. Tech. 3, 183-194.
[5]	Washio, S., Takahashi, S., Murakami K., Tada, T., Deguchi, S. (2008) Cavity generation by accelerated relative motions between solid walls contacting in liquid. J. Mech. Eng. Sci. 222, 1695-1706.
[6]	Sanada, T., Sato, A., Shirota, M., Watanabe, M. (2009) Motion and coalescence of a pair of bubbles rising side by side. Chem. Eng. Sci. 64, 2659-2671.
[7]	Terasaka, K., Sasada, Y., Kobayashi, D., Fujioka, S. (2011) Submilli-bubble dispersion from a slit orifice into water. J. Chem. Eng. Jpn. 44 140-145.
[8]	Kodama, T., Tomita, Y., Koshiyama, K., Blomley, M.J.K. (2006) Transfection effect of microbubbles on cells in superposed ultrasound waves and behavior of cavitation bubble. Ultrasound Med. Biol. 32, 905-914.
[9]	Koshiyama, K., Kodama, K., Yano, T., Fujikawa, S. (2006) Structural change of lipid bilayer and water penetration induced by shock wave: molecular dynamics simulations. Biophys J. 91, 2198-2205.
[10]	Schlicher, R.K., Radhakrishna, H., Tolentino, T.P., Apkarian, R.P., Zarnitsyn, V., Prausnitz, M.R. (2006) Mechanism of intracellular delivery by acoustic cavitation. Ultrasound Med. Biol. 32, 915-924.
[11]	Hallow, D.M., Mahajan, A.D., Prausnitz, M.R. (2007) Ultrasonically targeted delivery into endothelial and smooth muscle cells in ex vivo arteries. J. Control Release. 118, 285-293.
[12]	Koshiyama, K., Kodama, K., Yano, T., Fujikawa, S. (2008) Molecular dynamics simulation of structural changes of lipid bilayers induced by shock waves: Effects of incident angles. Biochim. Biophys. Acta. 1778, 1423- 1428.
[13]	Kodama, T., Tomita, Y., Watanabe, Y., Koshiyama, K., Yano, T., Fujikawa, S. (2009) Cavitation bubbles mediated molecular delivery during sonoporation. J. Biomech. Sci. Eng. 4, 124-140.
[14]	Kumon, R.E., Aehle, M., Sabens, D., Parikh, P., Han, Y.W., Kourennyi, D., Deng, C.X. (2009) Spatiotemporal effects of sonoporation measured by real-time calcium imaging. Ultrasound Med. Biol. 35, 494-506.
[15]	Koshiyama, K., Yano, T., Kodama, T. (2010) Self-organization of a stable pore structure in a phospholipid bilayer. Phys. Rev. Lett. 105, 018105.
[16]	Koshiyama, K., Wada, S. (2011) Molecular dynamics simulations of pore formation dynamics during the rupture process of a phospholipid bilayer caused by high- speed equibiaxial stretching. J. Biomech. 44, 2053-2058.
[17]	van Wamel, A., Bouakaz, A., Versluis, M., de Jong, N. (2004) Micromanipulation of endothelial cells: ultrasound-microbubble-cell interaction. Ultrasound Med. Biol. 30, 1255-1258.
[18]	van Wamel, A., Kooiman, K., Harteveld, M., Emmer, M., ten Cate, F.J., Versluis, M., de Jong, N. (2006) Vibrating microbubbles poking individual cells: drug transfer into cells via sonoporation. J. Control Release. 112, 149-155.
[19]	Taylor, R.S., Hnatovsky, C. (2004) Trapping and mixing of particles in water using a microbubble attached to an NSOM fiber probe. Opt. Express 12, 916-928.
[20]	Taylor, R.S., Hnatovsky, C. (2004) Growth and decay dynamics of a stable microbubble produced at the end of a near-field scanning optical microscopy fiber probe. J. Appl. Phys. 95, 8444-8449.
[21]	Diop, M., Taylor, R. (2006) Soft trapping and manipulation of cells using a disposable nanoliter biochamber. Biophys. J. 90, 3813-3822.