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Insights and relative effect of aspirin, naproxen and ibuprofen containing hydrogels: From design to performance as a functional dual capacity restorative material and build in free radical defense: In-vitro studies

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DOI: 10.4236/ojst.2014.42013    3,251 Downloads   4,507 Views   Citations

ABSTRACT

Restorative materials in the new era aim to be “bio-active” and long-lasting. It has been suggested that the anti-inflammatory activity of some non-steroidal anti-inflammatory drugs (NSAIDs) may be partly due to their ability to scavenge reactive oxygen species (ROS) and reactive nitrogen species (RNS), as well as to inhibit the respiratory burst of neutrophils triggered by various activating agents. As a part of our continuous interest of developing functional dual action restorative materials capable of being “bio-active” and long-lasting, we design and evaluate novel chitosan hydrogels containing krill oil (antioxidant containing material), naproxen, ibuprofen (non steroidal anti-inflammatory medication), aspirin (pain relieve medication and free radical scavengers) and combinations thereof (chitosan-H-krill oil, chitosan-H-krill oil-aspirin and chitosan-H-naproxen, chitosan-H-naproxen-krill oil, chitosan-H-krill oil-ibuprofen and chitosan-H-ibuprofen) as functional additive prototypes for further development of “dual function restorative materials”; secondly, determine their effect on the dentin bond strength of a composite and thirdly, evaluate the capability of newly designed hydrogels to play an integral role of “build in” free radical defense mechanism by using BSA solubility as a “molecular prototype” of the site of free radical attack in vitro. Materials and Methods: The above mentioned hydrogels were prepared by dispersion of the corresponding component in glycerol and acetic acid

with the addition of chitosan gelling agent. The surface morphology (SEM), release behaviors (physiological pH and also in acidic conditions), stability of the therapeutic agent-antioxidant-chitosan and the effect of the hydrogels on the shear bond strength of dentin were also evaluated. Results: The release of aspirin, ibuprofen and naproxen confers the added benefit of synergistic action of a functional therapeutic delivery when comparing the newly designed chitosan-based hydrogel restorative materials to the commercially available products alone. Neither the release of aspirin, ibuprofen or naproxen nor the antioxidant stability was affected by storage over a 6- month period. The hydrogel formulations have a uniform distribution of drug content, homogenous texture and yellow color (SEM study). All chitosan dentin treated hydrogels gave significantly (P < 0.05; non-parametric ANOVA test) higher shear bond values (P < 0.05) than dentin treated or not treated with phosphoric acid. The model protein (BSA) was adopted to evaluate the chitosan-based functional biomaterials as defense for undesired free radical formation under in vitro conditions. Conclusion: The added benefits of the chitosan treated hydrogels involved positive influence on the aspirin, ibuprofen and naproxen release, increased dentin bond strength as well as demonstrated in vitro “build in” free radical defense mechanism, therefore acting as a “proof of concept” for the functional multi-dimentional restorative materials with the build in free radical defense mechanism.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Perchyonok, V. , Reher, V. , Zhang, S. , Grobler, S. , Oberholzer, T. and Massey, W. (2014) Insights and relative effect of aspirin, naproxen and ibuprofen containing hydrogels: From design to performance as a functional dual capacity restorative material and build in free radical defense: In-vitro studies. Open Journal of Stomatology, 4, 73-83. doi: 10.4236/ojst.2014.42013.

References

[1] Lefer, L.A. (1966) Psychoanalytic view of a dental phenomenon. Contemporary Psychoanalysis, 2, 135.
[2] Fine, E.W. (1971) Psychological factors associated with non-organic TMJ dysfunction syndrome. British Dental Journal, 131, 402-427.
http://dx.doi.org/10.1038/sj.bdj.4802760
[3] Feinmann, C. and Harris, M. (1984) Psychogenic facial pain. 1. The clinical presentation. British Dental Journal, 156, 165-168.
http://dx.doi.org/10.1038/sj.bdj.4805298
[4] Feinmann, C. and Harris, M. (1984) Psychogenic facial pain. Management and prognosis. British Dental Journal, 156, 205-208.
[5] Aghabeigi, B., Feinmann, C., Glover, V., et al. (1993) Tyramine conjugation deficit in patients with chronic idiopathic temporomandibular joint and orofacial pain. Pain, 54, 159-163.
http://dx.doi.org/10.1016/0304-3959(93)90204-3
[6] Fundueanu, G., Constantin, M. and Ascenzi, P. (2008) Preparation and characterization of pH- and temperature-sensitive pullulan microspheres for controlled release of drugs. Biomaterials, 29, 2767-2775.
http://dx.doi.org/10.1016/j.biomaterials.2008.03.025
[7] Huynh, D.P., Nguyen, M.K., Pi, B.S., Kim, M.S., Chae, S.Y., Lee, K.C., et al. (2008) Functionalized injectable hydrogels for controlled insulin delivery. Biomaterials, 29, 2527-2534.
http://dx.doi.org/10.1016/j.biomaterials.2008.02.016
[8] Wang, Y.-C., Liu, X.-Q., Sun, T.-M., Xiong, M.-H. and Wang, J. (2008) Functionalized micelles from block copolymer of polyphosphoester and poly(3-caprolactone) for receptormediated drug delivery. Journal of Controlled Release, 128, 32-40.
http://dx.doi.org/10.1016/j.jconrel.2008.01.021
[9] Nakamura, K., Maitani, Y., Lowman, A.M., Takayama, K., Peppas, N.A. and Nagai, T. (1999) Uptake and release of budesonide from mucoadhesive, pH-sensitive copolymers and their application to nasal delivery. Journal of Controlled Release, 61, 329-335.
http://dx.doi.org/10.1016/S0168-3659(99)00150-9
[10] Mok, H., Park, J.W. and Park, T.G. (2008) Enhanced intracellular delivery of quantum dot and adenovirus nanoparticles triggered by acidic pH via surface charge reversal. Bioconjugate Chemistry, 19, 797-801.
http://dx.doi.org/10.1021/bc700464m
[11] He, C., Kim, S.W. and Lee, D.S. (2008) In situ gelling stimuli-sensitive block copolymer hydrogels for drug delivery. Journal of Controlled Release, 127, 189-207.
http://dx.doi.org/10.1016/j.jconrel.2008.01.005
[12] Nguyen, D.N., Raghavan, S.S., Tashima, L.M., Lin, E.C., Fredette, S.J., Langer, R.S., et al. (2008) Enhancement of poly(orthoester) microspheres for DNA vaccine delivery by blending with poly(ethylenimine). Biomaterials, 29, 2783-2793.
http://dx.doi.org/10.1016/j.biomaterials.2008.03.011
[13] Bae, Y. and Kataoka, K. (2006) Significant enhancement of antitumor activity and bioavailability of intracellular pH-sensitive polymeric micelles by folate conjugation. Journal of Controlled Release, 116, e49-e50.
http://dx.doi.org/10.1016/j.jconrel.2006.09.044
[14] Kim, J., Conway, A. and Chauhan, A. (2008) Extended delivery of ophthalmic drugs by silicone hydrogel contact lenses. Biomaterials, 29, 2259-2269.
http://dx.doi.org/10.1016/j.biomaterials.2008.01.030
[15] Tang, Y. and Singh, J. (2008) Controlled delivery of aspirin: Effect of aspirin on polymer degradation and in vitro release from PLGA based phase sensitive systems. International Journal of Pharmaceutics, 357, 119-125.
http://dx.doi.org/10.1016/j.ijpharm.2008.01.053
[16] Watanabe, M., Kawano, K., Toma, K., Hattori, Y. and Maitani, Y. (2008) In vivo antitumor activity of camptothecin incorporated in liposomes formulated with an artificial lipid and human serum albumin. Journal of Controlled Release, 127, 231-238.
http://dx.doi.org/10.1016/j.jconrel.2008.02.005
[17] Tamilvanan, S., Venkateshan, N. and Ludwig, A. (2008) The potential of lipid- and polymerbased drug delivery carriers for eradicating biofilm consortia on devicerelated nosocomial infections. Journal of Controlled Release, 128, 2-22.
http://dx.doi.org/10.1016/j.jconrel.2008.01.006
[18] Lee, M.-H., Lin, H.-Y., Chen, H.-C. and Thomas, J.L. (2008) Ultrasound mediates the release of curcumin from microemulsions. Langmuir, 24, 1707-1713.
http://dx.doi.org/10.1021/la7022874
[19] Connal, L.A., Li, Q., Quinn, J.F., Tjipto, E., Caruso, F. and Qiao, G.G. (2008) pH-responsive poly (acrylic acid) core cross-linked star polymers: Morphology transitions in solution and multilayer thin films. Macromolecules, 41, 2620-2626.
http://dx.doi.org/10.1021/ma7019557
[20] Kurkuri, M.D., Nussio, M.R., Deslandes, A. and Voelcker, N.H. (2008) Thermosensitive copolymer coatings with enhanced wettability switching. Langmuir, 24, 4238-4244.
http://dx.doi.org/10.1021/la703668s
[21] Chen, S., Li, Y., Guo, C., Wang, J., Ma, J., Liang, X., et al. (2007) Temperature-responsive magnetite/PEO-PPO-PEO block copolymer nanoparticles for controlled drug targeting delivery. Langmuir, 23, 12669-12676.
http://dx.doi.org/10.1021/la702049d
[22] Shah, N.M., Pool, M.D. and Metters, A.T. (2006) Influence of network structure on the degradation of photo-cross-linked PLA-b-PEG-b-PLA hydrogels. Biomacromolecules, 7, 3171-3177.
http://dx.doi.org/10.1021/bm060339z
[23] Nolkrantz, K., Farre, C., Brederlau, A., Karlsson, R.I.D., Brennan, C., Eriksson, P.S., et al. (2001) Electroporation of single cells and tissues with an electrolyte-filled capillary. Analytical Chemistry, 73, 4469-4477.
http://dx.doi.org/10.1021/ac010403x
[24] Nguyen, K.T. and West, J.L. (2002) Photopolymerizable hydrogels for tissue engineering applications. Biomaterials, 23, 4307-4314.
http://dx.doi.org/10.1016/S0142-9612(02)00175-8
[25] Roy, I. and Gupta, M.N. (2003) Smart polymeric materials: Emerging biochemical applications. Chemistry & Biology, 10, 1161-1171.
http://dx.doi.org/10.1016/j.chembiol.2003.12.004
[26] Katime, I., Novoa, R., de Apodaca, E.D. and Rodríguez, E. (2004) Release of theophylline and aminophylline from acrylic acid/n-alkyl methacrylate hydrogels. Journal of Polymer Science Part A: Polymer Chemistry, 42, 2756-2765.
http://dx.doi.org/10.1002/pola.20112
[27] Alam, H.B., Burris, D., DaCorta, J.A. and Rhee, P. (2005) Hemorrhage control in the battlefield: Role of new hemostatic agents. Military Medicine, 170, 63-69.
[28] Amiji, M.M. (1995) Permeability and blood compatibility properties of chitosan-poly (ethylene oxide) blend membranes for haemodialysis. Biomaterials, 16, 593-599.
http://dx.doi.org/10.1016/0142-9612(95)93856-9
[29] Barrera, D.A., Zylstra, E., Lansbury, P.T. and Langer, R. (1993) Synthesis and RGD peptide modification of a new biodegradable copolymer: Poly(lactic acid-co-lysin). Journal of the American Chemical Society, 115, 11010-11011.
http://dx.doi.org/10.1021/ja00076a077
[30] Baumann, H. and Faust, V. (2001) Concepts for improved regioselective placement of O-sulfo, N-sulfo, N-acetyl, and N-carboxymethyl groups in chitosan derivatives. Carbohydrate Research, 331, 43-57.
http://dx.doi.org/10.1016/S0008-6215(01)00009-X
[31] Beena, M.S., Chandy, T. and Sharma, C.P. (1995) Heparin immobilized chitosan-poly ethylene glycol interpenetrating network: Antithrombogenicity. Artificial Cells, Blood Substitutes and Biotechnology, 23, 175-192.
http://dx.doi.org/10.3109/10731199509117937
[32] Bordenave, L.C., Lbaquey, R., Bareille, F., Lefebvre, C., Lauroua, V., Guerin, F., Rouais, N., More, C., Vergnes, C. and Anderson, J.M. (1995) Endothelial-cell compatibility testing of 3 different pelletanes. Journal of Biomedical Materials Research, 27, 1367-1381.
http://dx.doi.org/10.1002/jbm.820271104
[33] Brown, M., Daya, M. and Worley, J. (2009) Experience with chitosan dressings in a civilian EMS system. Journal of Emergency Medicine, 37, 1-7.
http://dx.doi.org/10.1016/j.jemermed.2007.05.043
[34] Carreno-Gomez, B. and Duncan, R. (1997) Evaluation of the properties of soluble chitosan and chitosan microspheres. International Journal of Pharmaceutics, 148, 231-240.
http://dx.doi.org/10.1016/S0378-5173(96)04847-8
[35] Cenni, E., Ciapetti, G., Cervellati, M., Cavedagna, D., Falsone, G., Gamberini, S. and Pizzoferrato, A. (1996) Activation of the plasma coagulation system induced by some biomaterials. Journal of Biomedical Materials Research, 31, 145-148.
http://dx.doi.org/10.1002/(SICI)1097-4636(199605)31:1<145::AID-JBM16>3.0.CO;2-M
[36] Cerchiara, T., BLuppi, B., Bigucci, F., Petrachi, M., Orienti, I. and Zecchi, V. (2003) Controlled release of vancomycin from freeze-dried chitosan salts coated with different fatty acids by spray-drying. Journal of Micro-encapsulation, 20, 473-478.
http://dx.doi.org/10.1080/0265204031000094329

  
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