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Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS

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DOI: 10.4236/jacen.2013.23010    2,679 Downloads   4,594 Views  
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ABSTRACT

Protein expression patterns in the polychlorinated biphenyl (PCB)-degrading Rhodococcussp. strain ACS were examined following growth on two substrates capable of inducing the enantioselective biotransformation of PCBs via different degradation pathways. Eleven
inducible proteins were identified by SDS-PAGE and characterized by LC-MS/MS. Four of the peptides, a spore coat protein, an extracellular serine protease, a spoVP, and a molecular chaperonin from Bacillus subtilis, were identified as being unique to biphenyl-induced cells, whereas anextracellular serine protease from B. subtilis was identified as being unique to carvone-induced cells.
None of the peptides identified had sequences that corresponded to known dioxygenases or other PCB-degrading enzymes of this Gram- positive bacterium, suggesting that the identified induced proteins may be involved in either PCB degradation or adaptive responses that protect cells from toxicity.


Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

J. Kim, "Biphenyl- and carvone-induced protein expression patterns in Rhodococcus sp. ACS," Journal of Agricultural Chemistry and Environment, Vol. 2 No. 3, 2013, pp. 65-73. doi: 10.4236/jacen.2013.23010.

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