Quantitative detection of Streptococcus mutans from saliva using FTA^TM elute cards and real-time polymerase chain reaction

Sepideh Seghatoleslami, Lars Ohlsson, Kristina Hamberg, Peter Carlsson, Dan Ericson, Lennart Ljunggren

Department of Biomedical Laboratory Science, Malm? University, Malm?, Sweden.
Department of Cariology, Faculty of Odontology, Malm? University, Malm?, Sweden.

DOI: 10.4236/ajmb.2013.33019                

Abstract

Dental caries is a localized, transmissible, pathological infection process that ends up in the destruction of hard dental tissue. Numerous reports have shown the close relationship between salivary levels of Streptococcus mutans and dental caries. As S. mutans, is considered to be the principle etiological agent of dental caries, the development of a quick and convenient method for detection and quantification of these bacteria from patient saliva samples would simplify diagnosis and treatment. The purpose of this study was to compare a new means of quantifying bacteria using FTA^TM Elute cards and Real-Time Polymerase Chain Reaction to a conventional culture-based assay using oral S. mutans as a model sample. A total of 60 different saliva samples were investigated. The results show a significant negative correlation between the two methods, with a correlation coefficient of -0.577 (Spearman’s Correlation) and p < 0.01. The method demonstrates a high sensitivity, specificity and reliable quantitative results, covering a large range of bacterial concentrations.

Keywords

Streptococcus mutans; FTA^TM Elute Cards; Real-Time PCR

Conflicts of Interest

The authors declare no conflicts of interest.

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