Characterization of Virulence Factors in Enteroaggregative and Atypical Enteropathogenic Escherichia coli Strains Isolated from Children with Diarrhea

Thiago Azevedo Feitosa Ferro, Francyelle Costa Moraes, Andreia Meneses da Silva, Claude Porcy, Leandro Amorim Soares, Cristina Andrade Monteiro, Nyla Thyara Melo Lobão, Francisco Amazonas Assis de Mello, Valério Monteiro-Neto, Patrícia de Maria Silva Figueirêdo

Research Group of Endemic and Parasitic Diseases, CEUMA University, S?o Luis, Brazil.

DOI: 10.4236/aid.2012.24022                

Abstract

Enteroaggregative (EAEC) and atypical enteropathogenic (EPEC) Escherichia coli are important bacterial etiologic agents causing diarrhea among children. The aim of the present study was to examine the impact of virulence factors predisposes to diarrhea. In this study some virulence properties were examined on 11 EAEC and 8 EPEC strains identified by Polymerase Chain Reaction (PCR), isolated from stool samples of children were analyzed genotypically and phenoltypically for the prevalence of virulence factors. The most frequently detected factor was resistance to serum (94%), followed by curli fimbriae (78%), biofilm production (73%), and gene coding for Extended-Spectrum Beta-Lactamase (ESBL) (68%). EPEC isolates showed at least three of the evaluated properties, while EAEC isolates showed at least two. The prevalence of these virulence factors between the two strains showed no statistical difference. This study showed the heterogeneity of the virulence profile of the isolates of EAEC and atypical EPEC strains and suggests that this diversity may influence in the disease severity.

Keywords

E.coli; Diarrhea; Virulence Factors

Conflicts of Interest

The authors declare no conflicts of interest.

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