In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

Maria Helena Madruga Lima-Ribeiro, Ralph Santos-Oliveira, Mauricélia Firmino de Santana, Terezinha de Jesus Andreoli Pinto, Irene Satiko Kikuchi, Cheila Gonçalves Mothé, Luana Cassandra Breitenbach Barroso Coelho, Maria Tereza dos Santos Correia, Ana Maria dos Anjos Carneiro-Leão

Biochemistry Department, Federal University of Pernambuco, Recife, Brazil.
Departament of Morphology and Animal Physiology, Federal Rural University of Pernambuco, Recife, Brazil..
Laboratory of Immunopathology Keizo Asami (LIKA), Federal University of Pernambuco, Recife, Brazil.
Nuclear Engineering Institute, Laboratory of Nanoradiopharmaceuticals, Rio de Janeiro, Brazil.
Pharmacy Department, University of Sao Paulo, Sao Paulo, Brazil.
School of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

DOI: 10.4236/jcdsa.2012.22011                

Abstract

Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol^? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with ⁶⁰Co gamma rays in a dose of 7.549 kGy·h^–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.

Keywords

Gamma Radiation; Natural Products; Hemagglutinating Activity; Cramoll-1, 4

Conflicts of Interest

The authors declare no conflicts of interest.

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