Effects of different priming treatments and priming durations on germination percentage of parsley (Petroselinum crispum L.) seeds

Atilla Dursun, Melek Ekinci

.

DOI: 10.4236/as.2010.11003                

Abstract

The effects of different priming treatments and priming durations on germination percentage at different temperatures in parsley seeds were studied. The seeds were treated for 2, 4, 6 and 8 days with the PEG 6000 (–0.5 MPa, –1.0 MPa and –1.5 MPa), KNO3 (0.30 mol/L and 0.35 mol/L), Mannitol (0.50 mol/L and 0.60 mol/L) and hydropriming (12h, 24h, 36h and 48h) and unprimed (control). Germination studies were made at 5, 10, 15, 20 and 25°C. Percentage of germination at different temperatures was significantly affected by priming treatments. Hydropriming (12h, 24h and 36 h) and mannitol 0.60 mol/L at 2 day generally had the highest germination percentages. In general, the highest germination percentage with priming was determined at 10°C. It may be said that seed priming treatments increased seed germination percentage at both low and high temperatures. The highest germination percentages were observed in both hydropriming and mannitol treatments as compared with PEG and KNO3 treatments. The PEG and KNO3 (2 and 4 days) treatments were better than unprimed treatment in all of the temperatures.

Keywords

Parsley; Germination Percentage; Seed Priming; PEG; KNO3; Hydropriming

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chivasa, W., Harris, D., Chiduza, C., Nyamudeza, P. and Mashingaidze, A.B. (1998) Agronomic practices, major crops and farmer’s perceptions of the importance of good stand establishment in Musikavanhu Communal Area, Zimbabwe. Journal of Applied Science in Southern Africa, 4(2), 108-125.
[2]	Murungu, E.S., Chıduza, C., Nyamugafata, P., Clark, L.J. and Whalley, W.R. (2004) Effect of on-farm seed priming on emergence, growth and yield of cotton and Maize in a semi-arid area of Zimbabwe. Experimental Agriculture, 40(1), 23-36.
[3]	Harris, D. (1996) The effects of manure, genotype, seed priming, depth and date of sowing on the emergence and early growth of Sorghum bicolor (L.) Moench in semi-arid Botswana. Soil and Tillage Research, 40(1-2), 73-88.
[4]	Nascimento, W.M. and Aragao, F.A.S. (2004) Muskmelon seed priming in relation to seed vigor. Scientia Agricola, 61(1), 114 -117.
[5]	Black, M. and Bawley, J.D. (2000) Seed technology and its biological basis. Sheffield Academic Press, Sheffield.
[6]	Duman, İ. (2005) Olumsuz çevre koşullarında tohumların çimlenme ve çıkış performansı nasıl artırılabilir? Hasad Dergisi, 21(246), 72-78.
[7]	Vural, H., Eşiyok, D. and Duman, İ. (2000) Kültür sebzeleri (sebze yetiştirme). Ege Üniversitesi Ziraat Fakül- tesi Bahçe Bitkileri Bölümü, Bornova-İzmir, 440.
[8]	Günay, A. (2005) Sebze yetiştiriciliği cilt II. İzmir, 531.
[9]	Cantliffe, D.J. (2003) Seed enhancement. ISHS Acta Horticulturae 607: IX International Symposium on Timing of Field Production in Vegetable Crops, Sao Paulo, 13 May 2003, 34(1).
[10]	Duman, İ. and Eşiyok, D. (1998) Ekim öncesi PEG ve KH2PO4 uygulamalarının havuç tohumlarının çimlenme ve çıkış oranı ile verim üzerine etkileri. Turkish Journal of Agriculture and Forestry, 22, 445-449.
[11]	Lee, J.M. (2004) Advances in seed treatments for horticultural crops. Chronica Horticulturae, 44(2), 11-20.
[12]	Barlow, E.W.R. and Haigh, A.M. (1987) Effect of seed priming on the emergence, growth and yield of UC 82B tomatoes in the field. ISHS Acta Horticultrae 200: II. International Symposium on Processing Tomatoes, XXII IHC, Davis, 1 March 1987, 22(1).
[13]	Zheng, G.H., Wilen, R.W., Slinkard, A.E. and Gusta, L.V. (1994) Enhancement of canola seed-germination and seedling emergence at low temperature by priming. Crop Science, 34(6), 1589-1593.
[14]	YeonOk, J., SeongMo, K. and JeoungLai, C. (2000) Priming conditions to improve germination of salvia (Salvia splendens F.) seeds. Korean Journal of Horticultural Science and Technology, 18(2), 98-102.
[15]	YeonOk, J., JongCheol, K. and JeoungLai, C. (2000) Effect of priming duration and temperature on the germinability of carrot, lettuce, onion, and welsh onion seeds. Korean Journal of Horticultural Science and Technology, 18(3), 327-333.
[16]	Nascimento, W.M. (2003) Muskmelon seed germination and seedling development in response to seed priming. Scientia Agricola, 60(1), 71-75.
[17]	Perezgarcia, F., Pita, J.M., Gonzalezbenito, M.E. and Irıondo, J.M. (1995) Effects of light, temperature and seed priming on germination of celery seeds (Apium graveolens L.). Seed Science and Technology, 23(2), 377-383.
[18]	Nascimento, W.M. (2005) Vegetable seed priming to improve germination at low temperature. Horticultura Brasileria, 23(2), 211-214.
[19]	Elkoca, E., Haliloğlu, K., Eşitken, A. and Ercişli, S. (2007) Hydro and osmopriming improve chickpea germination. Acta Agriculturae Scandinavica Section B Soil and Plant Science, 57(3), 193-200.
[20]	Olszweski, M.W., Evans, T.A., Gregory, N.F. and Pill, W.G. (2005) Enhanced germination of primed mericarps of parsley (Petroselinum crispum Mill. Nyman ex A.W.Hill) limited by Alternaria alternate proliferation. Journal of Horticultural Science and Biotechnology, 80(4), 427-432.
[21]	Olszewski, M., Pill, W., Pizzolato, T.D. and Pesek, J. (2005) Priming duration influences anatomy and germination responses of parsley mericarps. Journal of the American Society for Horticultural Science, 130(5), 754- 758.
[22]	Pill, W.G. and Kilian, E.A. (2000) Germination and emergence of parsley in response to osmotic or matric seed priming and treatment with gibberellin. Horticultural Science, 35(5), 907-909.
[23]	Duman, İ. (2002) Soğan (Allium cepa L.) tohumlarının çimlenmesini iyileştirici farklı osmotik uygulama yöntemlerinin karşılaştırılması. Ege Üniversitesi Ziraat Fakültesi Dergisi, 39(2), 1-8.
[24]	Yıldırım, E., Dursun, A., Güvenç, İ. and Kumlay, A.M. (2002) Effects of different salt, biostumulant and temperature levels on seed germination of some vegetable species. Acta Agrobotanica, 55(2), 75-80.
[25]	Demir, İ. and Mavi, K. (2008) Effect of salt and osmotic stresses on the germination of pepper seeds of different maturation stages. Brazilian Archives of Biology and Technology, 51(5), 897-902.
[26]	Demir, İ. and Mavi, K. (2004) The effect of priming on seedling emergence of differentially matured watermelon (Citrullus lanatus (Thunb.) Matsum and Nakai) seeds. Scientia Horticulturae, 102(4), 467-473.
[27]	Arin, L. and Kiyak, Y. (2003) The effects of pre-sowing treatments on emergence and seedling growth of tomato seed (Lycopersicon esculentum Mill.) under several stress conditions. Pakistan Journal of Biological Sciences, 6(11), 990-994.
[28]	Corbineau, F., Picard, M.A. and Come, D. (1994) Germinability of leek seeds and its improvement by osmopriming. ISHS Acta Horticulture 371: VII International Symposium on Timing Field Production of Vegetables, Skierniewice, 1 July 1994, 57(1).
[29]	Khalil, S.K., Mexal, J.G. and Murray, L.W. (2001) Germination of soybean primed in aerated solution of polyethylene glycol (8000). Online Journal of Biological Sciences, 1(3), 105-107.
[30]	Pazdera, J. and Hosnedl, V. (2002) Effect of hydration treatments on seed parameters of different lettuce (Lactuca sativa L.) seed lots. Horticultural Science, 29(1), 12-16.
[31]	Sivritepe, H.Ö. and Eris, A. (2000) The effects of post- storage priming treatments on viability and repair of genetic damage in pea seeds. ISHS: XXV International Horticultural Congress, Part 7: Quality of Horticultural Products, 517, 143-149.
[32]	Sadeghian, S.Y. and Yavari, N. (2004) Effect of water- deficit stress on germination and early seedling growth in sugar beet. Journal of Agronomy and Crop Science, 190(2), 138-144.
[33]	Dhillon, N.P.S. (1995) Seed priming of male sterile muskmelon (Cucumis melo L.) for low temperature germination. Seed Science and Technology, 23(3), 881- 884.
[34]	Demir, İ., Ellialtıoğlu, S. and Tipirdamaz, R. (1993) The effect of different priming treatments on reparability of aged eggplant seeds. ISHS: International Symposium on Agrotechnics and Storage of Vegetable and Ornamental Seeds, Bari, 14-16 June 1993, 362, 205-212.
[35]	Brocklehurst, P.A., Dearman, J. and Drew, R.L.K. (1987) Recent developments in osmotic treatment of vegetable seeds. ISHS Acta Horticulturae 215: Seed Research in Horticulture, 30(1).
[36]	İlbi, H. and Eser, B. (2004) The effects of seed treatments on ageing in onion seed. Ege Üniversitesi Ziraat Fakültesi Dergisi, 41(1), 39-48.
[37]	Sivritepe, H.Ö., Eris, A. and Sivritepe, N. (1999) The effect of NaCl priming on salt tolerance in melon seedling. ISTA: I International Symposium on Cucurbits, Adana, 1 May 1999, 492, 77-84.
[38]	Sivritepe, H.Ö., Eris, A. and Sivritepe, N. (1999) The effect of priming treatments on salt tolerance in melon seeds. ISTA: I International Symposium on Cucurbits, Adana, 1 May 1999, 492, 287-295.
[39]	Sivritepe, H.Ö., Sivritepe, N., Eriş, A. and Turhan, E. (2005) The effects of NaCl pre-treatments on salt tolerance of melon grown under long-term salinity. Science Horticulturae, 106(4), 568-581.