Molecular Characterization of Pseudomonas spp. Isolated from Root Nodules of Various Leguminous Plants of Shekhawati Region, Rajasthan, India

Sakshi Issar, Saroj Sharma, Devendra Kumar Choudhary, Hemant Kumar Gautam, R. K. Gaur

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DOI: 10.4236/ajps.2012.31005                

Abstract

Plant growth promontory Pseudomonas strains were isolated from root nodules of five plant species, viz., Trifolium pretense, Cicer arietinum, Amaranthus polygamus, Vigna mungo, and Trigonella foenum; that plants were denizen of Shekhawati region of Rajasthan. A total of 8 bacterial isolates were evaluated for growth promotion using PGP properties. Partial 16S rDNA sequencing data showed that these 8 bacterial isolates belonged to genus Pseudomonas. MEGA 4.0.2, software was used to construct a neighbor joining tree by employing boot strap method. Result exhibited significant diversity among recovered Pseudomonas strains.

Keywords

PGPRs; 16S Sequencing; Pseudomonas; Root-Nodule Bacteria; 16S rDNA

Conflicts of Interest

The authors declare no conflicts of interest.

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