Effect of static magnetic field on erythrocytes characterizations

Mohamed A. Elblbesy

.

DOI: 10.4236/jbise.2010.33040                

Abstract

The interaction of static magnetic field (SMF) with living organisms is a rapidly growing field of investigation. Recently, exposure to moderate intensity SMFs (1 mT – 1 T) has attracted much attention for its various medical applications. This study was designed to show the microscopic effect of SMF on erythrocytes in vitro. For this purpose SMF system was constructed in my lab on basis of the idea of cell tracking velocimetry system. The changes in erythrocytes surface area, sphericity, and adhesion number for erythrocytes were calculated to quantify the effect of SMF on erythrocytes characterizations. The results showed that SMF increased erythrocytes surface area and reduced their sphericity. The adhesion number of erythrocytes under the influence of SMF showed the tendency of erythrocytes to adhere with each other. These findings indicate that more study on microscopic scales must be carried out in order to investigate the effect of SMF on erythrocytes.

Keywords

Static Magnetic Field; Erythrocytes; Surface Area; Shericity, Adhesion

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Higashi, T., Ashida, N. and Takeuchi, T (1997) Orientation of blood cells in static magnetic field. Physica B, 237-238, 616-620.
[2]	Terumasa, H., Akio, Y., Tetsuya, T. and Muneyuki D. (1995) Effects of static magnetic fields on erythrocyte rheology. Bioelectrochemistry and Bioenergetics, 36, 101-108.
[3]	Repacholy, M.H. (2001) Review of health effects and gaps in knowledge. Proceedings of WHO Meeting on EMF Biological Effects and Standard Harmonization in Asia and Okeania, Seoul, 33-42.
[4]	Hakobyan, S., Baghdasaryan, N., Amyan, A. and Ayrapetyan, S. (2001) The effect of EMF on water specific electrical conductivity and wheat sprouting. Proceedings of WHO Meeting on EMF Biological Effects and Standard Harmonization in Asia and Okeania, Seoul, 123.
[5]	Rosen, A.D. (2003) Mechanism of action of moderate-intensity static magnetic fields on biological systems. Cellular Biochemistry Biophysics, 39, 163-173.
[6]	Lee, R.M., Maciej, Z., Masayuki, N., Kara, M., Sigalit, G., Merav, Z., Shlomo, M. and Jeffrey, J.C. (2000) The use of magnetite-doped polymeric microspheres in calibrating cell tracking velocimetry. Journal of Biochemical and Biophysical Methods, 44(1-2), 115-130.
[7]	Canham, P.B. and Burton, A.C. (1968) Distribution of size and shape in populations of normal human red cells. Circulation Research, 22, 405-422.
[8]	Lisi, A., Pozzi, D., Pasquali, E., Rieti, S., Girasole, M., Cricenti, A., Generosi, R., Serafino, A.L., Congiu- Castellano, A., Ravagnan, G., Giuliani, L. and Grimaldi, S. (2000) Three dimensional (3D) analysis of the morphological changes induced by 50 Hz magnetic field exposure on human lymphoblastoid cells (Raji). Bioelectromagnetics, 21, 46-51.
[9]	Chionna, A., Dwikat, M., Panzarini, E., Tenuzzo, B., Carla`, E.C., Verri, T., Pagliara, P., Abbro, L. and Dini, L. (2003) Cell shape and plasma membrane alterations after static magnetic fields exposure. European Journal of Histochemistry, 47, 299-308.
[10]	Rieti, S., Manni, V., Lisi, A., Giuliani, L., Sacco, D., D’Emilia, E., Cricenti, A., Generosi, R., Luce, M. and Grimaldi, S. (2004) SNOM and AFM microscopy techniques to study the effect of non-ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT). Journal of Microscopy, 213, 20-28.
[11]	Dilek, U.C., Beran, Y., Mehmet, Z.A., Cemil, S. and Nuriye, M. (2009) Alterations of hematological variations in rats exposed to extremely low frequency magnetic fields (50 Hz). Archives of Medical Research, 40, 352-356.
[12]	Sanjay, J. and Megha, S. (2002) Influence of an inhomogeneous magnetic field on erythrocyte aggregation mechanism an analysis by He–Ne laser aggregometer. Journal of Magnetism and Magnetic Materials, 252, 412-414.