Author(s)

Guangnan Ou^1,2*, Biyan He^1,2

¹College of Food and Biological Engineering, Jimei University, Xiamen, China.
²Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, China.

Ionic liquids (ILs) with buffering and chelating abilities were designed and synthesized on the basis of ethylenediaminetetraacetic acid (EDTA) for the development of buffered enzymatic IL systems and for enzymatic reaction in heavy metal containing aqueous system. Transesterification activity of Candida antarctica lipase B dissolved in the hydroxyl-functionalized IL was buffer dependent. High activity and outstanding stability was obtained with the buffered enzymatic IL systems for the transesterification. In heavy metal containing aqueous system, EDTA IL buffers as Hg²⁺ chelators protected horseradish peroxidase (HRP) against Hg²⁺-induced denaturation and precipitation. Higher pH favored the protection, while at lower pH the protection diminished. We can conclude that the new ILs possess both buffering and chelating abilities.

Ionic Liquid, EDTA, Buffer, Chelator, Lipase, Peroxidise, Heavy Metal

Ou, G. and He, B. (2019) New Ionic Liquids with Buffering and Chelating Abilities for Enzyme Engineering. Advances in Bioscience and Biotechnology, 10, 320-330. doi: 10.4236/abb.2019.1010025.