Author(s)

Akane Yoshizaki¹, Toshiko Tomisawa², Tomohiro Osanai², Naoki Nanashima³, Maiko Kitajima², Kasumi Mikami², Toshifumi Fujita⁴, Hayato Maeda⁵, Yoji Kato⁶

¹Hirosaki University Medical Hospital, Hirosaki, Japan.
²Department of Nursing Science, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
³Department of Biomedical Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
⁴Department of Disability and Health, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan.
⁵Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan.
⁶Faculty of Education, Hirosaki University, Hirosaki, Japan.

Objectives: Smoking causes endothelial dysfunction, but it still remains unclear whether oral administration of anthocyanin brings benefits to endothelial function and redox states in young healthy smokers. We tested the hypothesis that supplement anthocyanin may rescue endothelial dysfunction and redox states in young healthy smokers. Methods: Young healthy male non-smoker (n = 8) and smokers (n = 14) were enrolled to measure the derivatives of reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) levels and brachial artery flow-mediated dilation (FMD) before and 2 hours after one cigarette smoking or resting under administration of blackcurrant supplements (anthocyanins 210 mg) or not. Results: FMD, d-ROMs, and BAP at baseline were similar between non-smokers and smokers. One cigarette smoking induced a decrease in FMD at 2 hours (9.0% ± 2.6% vs 7.5% ± 2.3%, p < 0.05) without affecting plasma levels of d-ROMs and BAP in young healthy smokers. Single oral administration of anthocyanin at 210 mg abolished smoking-induced decrease in FMD (8.3% ± 3.4% vs 9.5% ± 3.6%, p = ns) without changes in plasma levels of d-ROMs and BAP in young smokers. In non-smokers, however, administration of anthocyanin at 210 mg slightly but significantly elevated plasma level of d-ROMs at 2 hours (249.6 ± 30.3 vs 265.5 ± 36.3 U CARR, p < 0.05) without affecting plasma level of BAP and FMD. Conclusions: Single oral administration of anthocyanin rescued smoking-induced endothelial dysfunction in young healthy smokers, but facilitated oxidative stress in non-smokers. When anthocyanin is taken as supplements, it seems important to pay attention to the dose and timing of administration.

Blackcurrant, Anthocyanin, Young Health Smoker, Oxidative Stress, Antioxidants

Yoshizaki, A. , Tomisawa, T. , Osanai, T. , Nanashima, N. , Kitajima, M. , Mikami, K. , Fujita, T. , Maeda, H. and Kato, Y. (2018) Single Oral Administration of Anthocyanin Rescues Smoking-Induced Endothelial Dysfunction in Young Smokers but Facilitates Oxidative Stress in Non-Smokers. Food and Nutrition Sciences, 9, 179-190. doi: 10.4236/fns.2018.93014.