Author(s)

Madhurima Kahali, Kamlesh Kumar Soni, Pradeep Kumar Burma^*

Department of Genetics, University of Delhi South Campus, New Delhi, India.

Developing transgenics that express high levels of Cry1Ac protein, and at the same time, are phenotypically normal, has not been an easy task to achieve. It has been routinely observed that most of the transgenic plants that survive, show no or extremely low levels of Cry1Ac protein. However, all of these plants do express the selectable marker, nptII gene. In the present study, we record an interesting observation of how one of the genes (cry1Ac) on a single T-DNA fragment is selectively silenced, keeping the expression of the other gene (nptII) intact. Further, this silenced state is inherited.

Cry1Ac, Transcriptional Silencing, Bt Crops, Gene Silencing

Kahali, M. , Kumar Soni, K. and Kumar Burma, P. (2017) Studies on Trans-Generational Transcriptional Silencing of cry1Ac Gene in Tobacco Transgenics. American Journal of Molecular Biology, 7, 1-10. doi: 10.4236/ajmb.2017.71001.