Author(s)

Masataka Yoshikawa^*, Hideyuki Kakigi, Ayano Miyamoto, Sadaomi Sugimoto, Keisuke Nakai, Hideaki Ikenaga, Takeshi Inamoto, Hiroshi Maeda

Department of Endodontics, Osaka Dental University, Osaka, Japan.

A bi-phasic scaffold consisting of a columnar formaldehyde-acetalized polyvinyl alcohol (PVF) sponge and a cylindrical porous hydroxyapatite (HA) with a hollow center was devised. Rat bone marrow cells (rBMCs) were seeded into the sponge placed in the hollow center of the cylindrical porous HA. The bi-phasic scaffold, a cylindrical porous HA and a PVF sponge separated from a bi-phasic scaffold after rBMC seeding, and a PVF sponge without rBMCs as a negative control, were implanted for 6 weeks into rat dorsal subcutaneous tissue. In each construct, bone formation was examined histologically and osteocalcin was measured immunochemically. Bone formation was observed in the bi-phasic scaffold and also in the cylindrical porous HA isolated from the bi-phasic scaffold. A significant difference in the quantity of osteocalcin was observed between the bi-phasic scaffold and the isolated cylindrical porous HA. No bone formation was found in the isolated PVF sponge. The bi-phasic scaffold as an outer layer of the scaffold seemed to inhibit the outflow of rBMCs from the PVF sponge. This type of bi-phasic scaffold may have two specific characteristics: Attachment of cells both in PVF sponge and cylindrical porous HA.

Bi-Phasic Scaffold, Hydroxyapatite, Polyvinyl Alcohol Sponge, Bone Formation, In Vivo

Yoshikawa, M. , Kakigi, H. , Miyamoto, A. , Sugimoto, S. , Nakai, K. , Ikenaga, H. , Inamoto, T. and Maeda, H. (2016) In Vivo Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components. Journal of Biomedical Science and Engineering, 9, 501-514. doi: 10.4236/jbise.2016.911045.