Analysis of the Liver of Fish Species Prochilodus lineatus Altered Environments, Analyzed with ImageJ - Microscopy Research

MR > Vol.5 No.1, January 2017

Analysis of the Liver of Fish Species Prochilodus lineatus Altered Environments, Analyzed with ImageJ ()

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DOI: 10.4236/mr.2017.51001 1,985 Downloads 3,753 Views Citations

Author(s)

Vanessa Ellen Wendt Campos¹, Bruno Fiorelini Pereira^2*, Dimítrius Leonardo Pitol³, Rebeca Mamede da Silva Alves¹, Flavio Henrique Caetano¹

Affiliation(s)

¹Department of Biology, Institute of Biosciences Rio Claro, Sao Paulo State University, Sao Paulo, Brazil.
²Universidade Federal do Oeste da Bahia, Barreiras-BA, Brazil.
³Department of Morphology, Physiology and Basic Pathology, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil.

ABSTRACT

The fish Prochilodus lineatus (Characiformes, Prochilodontidae), in addition to being a good bioindicator, is also of economic and ecological importance with a broad distribution in the neotropics. Ecotoxicology examines the interaction between environmental chemistry and biota; and in this study we assess alterations of bile and glycogen levels in the fish liver, organ responsible for detoxification, biotransformation and storing nutrients, such as glycogen, and for secreting bile. Fish were separated in three groups to examine the damage caused by the exposure to waters from Lago Azul-Rio Claro-SP and containing diluted biodegradable detergents in comparison to a control group (chlorinated water from an artesian well of UNESP-Campus Rio Claro). A histological analysis was performed on HE and PAS stained sections. The identification of structural changes and the assessment of the area occupied by bile and glycogen were carried out with the software ImageJ, showing that the liver was affected morphologically (cell vacuolization, peripherals nuclei, for example) and problems in bile release and production and storage of glycogen.

KEYWORDS

Liver, Glycogen, Bile, Prochilodus lineatus

Share and Cite:

Wendt Campos, V. , Pereira, B. , Pitol, D. , da Silva Alves, R. and Caetano, F. (2017) Analysis of the Liver of Fish Species Prochilodus lineatus Altered Environments, Analyzed with ImageJ. Microscopy Research, 5, 1-9. doi: 10.4236/mr.2017.51001.

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