In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum - American Journal of Plant Sciences

AJPS > Vol.7 No.9, July 2016

In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum ()

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Download as PDF (Size: 667KB) PP. 1317-1324

DOI: 10.4236/ajps.2016.79126 2,139 Downloads 4,019 Views Citations

Author(s)

Himanshu Saini^1,2, Inder Dev Arya¹, Sarita Arya¹, Reetu Sharma¹

Affiliation(s)

¹Tissue Culture Discipline, Botany Division, Forest Research Institute, Dehradun, India.
²Genetic Resource Center, International Institute of Tropical Agriculture, HQ & West Africa Hub, Ibadan, Nigeria.

ABSTRACT

Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1-naphthalene acetic acid) and IBA (indole-3 butyric acid) effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum. In vitro micropropagation of himalayan weeping bamboo is explained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surface sterilized with 0.1% HgCl₂ for 10 - 12 minutes, cleaned with 90% ethanol and inoculated on liquid Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP/ KIN. Effect of BAP/KIN on shoot induction is with different rate and number of shoots produced by explants with axillary bud cultured on MS media supplemented with 0.0 mg/L BAP/KIN - 5.5 mg/L BAP/KIN. Shoot multiplication with highest rate is achieved on MS medium supplemented with 3.5 mg/L BAP after 4^th sub-culturing. The most effective with highest rate and number of root induction combination is 6.5 mg/L IBA after 5 weeks. The roots produced by 6.5 mg/L IBA is best compared with other combination of auxin NAA (1-naphthalene acetic acid).

KEYWORDS

In Vitro, Drepanostachyum falcatum, Himalayan Weeping Bamboo, Plant Growth Hormone, Micropropagation

Share and Cite:

Saini, H. , Arya, I. , Arya, S. and Sharma, R. (2016) In Vitro Micropropagation of Himalayan Weeping Bamboo, Drepanostachyum falcatum. American Journal of Plant Sciences, 7, 1317-1324. doi: 10.4236/ajps.2016.79126.

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