ABSTRACT
Purpose: Our study was to detect the effect of Decitabine
(DAC) combined with arsenic trioxide (AS2O3) on DAPK gene
and HL-60 cell proliferation and apoptosis. Methods: DAC and AS2O3 monotherapy,
combination treatment and DAC pretreatment were used in this study after incubating
with HL-60 cell for 24 h, 48 h, 72 h. CCK8 was used to detect the cell
proliferation of HL-60 cell. Flow cytometry was used to detect the cell
apoptosis. Then, we used RT-PCR to obtain the gene expression level of DAPK. Results: HL-60 cells were treated
with different concentrations of DAC (20 μmol/L, 40 μmol/L, 80 μmol/L), AS2O3 (1 μmol/L, 2.5 μmol/L, 5 μmol/L) monotherapy for 24 h, 48 h, 72 h; along with
the extension of the drug concentration and time, proliferation inhibition rate
had gradually increased. Monotherapy of DAC, AS2O3 could
inhibit the proliferation and induce
apoptosis of HL-60 cells, and was time- and dose-dependent. DAC (80 μmol/L) was
firstly used for pretreatment, and then, different concentrations of AS2O3 (1 μmol/L, 2.5 μmol/L, 5 μmol/L) were used for 24 h, 48 h, 72 h. It was
found that cell proliferation inhibition rate and apoptosis rate had increased
significantly. When the two drugs were used together, the increasing proliferation
inhibition rate, apoptosis rate and DAPK had become more obvious. Conclusion: DAC and AS2O3 had a synergetic effect for the HL-60 cell proliferation inhibition, apoptosis
and expression of DAPK.